Involvement of a goose-type lysozyme from Takifugu obscurus in immune response against Gram-negative bacteria
2025
Ying Huang | Rui Shen | Li-Fan Cui | Yan Shi | Xiao-Rui Song | Zhe Zhao
Lysozyme plays a crucial role in the immune response to bacterial infections. In this research, a goose-type lysozyme (designed as ToLyzG) was identified from Takifugu obscurus. ToLyzG cDNA encoded a 190-amino-acid protein. The phylogenetic tree showed that ToLyzG was primarily grouped with five g-type lysozymes from other pufferfish. ToLyzG was expressed in all examined tissues of untreated T. obscurus. The expression of ToLyzG in the liver, spleen, kidney, and intestine could be induced after Vibrio harveyi infection. To understand its biological activity, the coding region of ToLyzG was expressed and purified. The optimal conditions for recombinant ToLyzG were pH 7.0 and 35 °C. rToLyzG had lytic activity against a variety of bacteria with the highest activity against V. harveyi, Vibrio parahaemolyticus, and Aeromonas hydrophila. rToLyzG protein could not only bind to lipopolysaccharide, peptidoglycan, and six species of bacteria, but also inhibit the growth of these bacteria. Remarkably, rToLyzG demonstrated more potent suppressive activity against Gram-negative bacteria than Gram-positive bacteria. The present research enhances the comprehension of the biochemical features of g-type lysozyme from T. obscurus, potentially shedding light on its significant function in the host's defense strategy against bacterial pathogens.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par Maximum Academic Press
Découvrez la collection de ce fournisseur de données dans AGRIS