Trimethylamine N-oxide promotes the growth of Chinese mitten crab (Eriocheir sinensis), deposition of amines, and activates the mTOR pathway by affecting metabolism of amino acids
2025
Haokun Hua | Huixing Guo | Wenbin Liu | Zishang Liu | Chaofan He | Yangyang Huang | Xiufei Cao | Wei Xiong | Xi Wang | Yubo Wen | Saiwei Yin | Pengbo Wang | Aimin Wang | Guangzhen Jiang
This study was conducted to evaluate the changes in amino acid metabolism and amine deposition in Chinese mitten crab (E. sinensis) fed with a diet containing Trimethylamine N-oxide (TMAO) to promote its growth and flavor. Three diets containing 0% TMAO, 0.01% TMAO, and 0.04% TMAO were designed respectively for E. sinensis (37.50 ± 0.5 g) for 15 weeks. According to the results, two experimental groups with TMAO had significant decrease in feed conversion ratio and feed intake (p < 0.05) while it showed the opposite trend in the enzyme activity of trypsin. With the addition of TMAO, the value of umami in muscle increased significantly (p < 0.05). Compared with the other two groups, the 0.04% TMAO group showed a significant increase in TMAO deposition and the content of methionine in the muscle (p < 0.05). Meanwhile, the deposition of spermidine and spermine significantly decreased in this group (p < 0.05). When feeding crabs with the diet containing 0.01% TMAO, the relative gene expression of S6K1 and eIF4E increased significantly while 4E-BP1 decreased markedly (p < 0.05). The protein expression of mTOR in muscle showed an upward trend in the 0.01% TMAO group. In summary, 0.01% TMAO in the diet could improve the FCR by increasing the enzyme activity of trypsin and improving growth performance by activating the mTOR pathway. 0.04% TMAO could increase the value of umami and the deposition of TMAO in muscle.
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