Data on genetic dissection and QTL mapping of fodder quality traits in Sorghum under different water regimes
Somegowda K Vinutha | Vemula Anilkumar | Naravula Jalaja | Rathore Abhishek | Jones Chris | Deshpande P Santosh | Deshpande, Santosh
Data on QTL analysis and identification of genomic regions or loci associated with fodder quality traits in sorghum. The genotypes along with parents were sown and DNA was isolated from leaves of each accession at 4 to 6 leaf stage using the modified Hexadecyltrimethylammonium bromide (CTAB) protocol from 12-day-old seedlings and genotyping done following the GBS (genotyping by sequencing) approach with ApeKI restriction enzyme used for complexity reduction. SNPs were called using TASSEL v5.2 GBS pipeline against sorghum assembly v3.1 with default parameters. The raw SNP data has 321 taxa (including population and two parents) with 292960 numbers of sites. The SNP calling was done at 90% coverage based on sites, >10% missing values, and minor allele frequencies <5% were excluded from further analysis. The final marker count was 1141 markers and BLUPs data generated from phenotyping was used to identify significant QTL for fodder quality under drought conditions.
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