Physiological and biochemical changes in Matricaria chamomilla induced by Pseudomonas fluorescens and water deficit stress | FIZIOLOŠKE IN BIOKEMIČNE SPREMEMBE PRAVE KAMILICE (Matricaria chamomilla L.) VZPODBUJENE Z BAKTERIJO Pseudomonas fluorescens IN POMANKANJEM VODE

anglais. Environmental stresses and rhizosphere microorganisms affect growth parameters and accumulation of active ingredients especially in plants with medicinal properties. The present study examined the effects of chamomile (Matricaria chamomilla L.) seedling inoculation with Pseudomonas fluorescens PF-135 strain on its growth parameters, photosynthetic pigments, proline, malondialdehyde (MDA), and hydrogen peroxide (H2O2) content, and essential oil concentration at both regular watering and water deficit experiments. Based on the obtained results, water deficit stress reduced root dry mass, and flower fresh and dry mass as well. However, amount of H2O2 and MDA in root and shoot tissues were considerably lower in inoculated plants compared to non-inoculated ones under both normal watering and water deficit regimes. It indicates that lipid peroxidation and production of reactive oxygen species has been diminished in inoculated plants. Also, essential oil content in inoculated plants significantly increased compared with that of non-inoculated ones under water deficit stress condition. It can be concluded that P. fluorescens PF-135 strain has an outstanding potential to alleviate adverse effects of water deficit on plant growth, and hence can be used as an excellent PGPR in order to boost chamomile productivity especially under water deficit stress condition.

slovène. Okoljski stresi in mikroorganizmi v rizosferi vplivajo na rastne parametre in na kopičenje aktivnih snovi, še posebej v rastlinah z zdravilnimi lastnostmi. V raziskavi so bili preučevani učinki inokulacije sejank kamilice (Matricaria chamomilla L.) s sevom bakterije Pseudomonas fluorescens PF-135 na njene rastne parametre, vsebnost fotosinteznih barvil, prolina, malondialdehida (MDA), vodikovegan peroksida (H2O2) in eteričnih olj v razmerah rednega zalivanja in ob pomanjkanju vode. Rezultati so pokazali, da je pomanjkanje vode zmanjšalo suho maso korenin in svežo ter suho maso cvetov. Količini H2O2 in MDA v tkivih korenin in poganjkov sta bili manjši pri inokuliranih rastlina v primerjavi z neinokuliranimi pri obeh vodnih režimih. To kaže, da je bila peroksidacija lipidov in tvorba reaktivnih zvrsti kisika manjša pri inokuliranih rastlinah. Tudi vsebnost eteričnioh olj se je pri inokuliranih rastlinah značilno povečala v primerjavi z neinokuliranimi v razmerah pomanjkanja vode. Zaključili bi lahko, da ima sev bakterije P. fluorescens PF-135 izjemen potencial za odpravljanje škodljivih učinkov pomanjkanja vode na rast rastlin in bi bil lahko uporabljen kot odlična PGPR snov za povečanje pridelka kamilice, še posebej v razmerah pomanjkanja vode.