Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars | Primerjalno preverjanje ISSR, DAMD in RAPD markerjev za vrednotenje genetske raznolikosti sort gerbere (Gerbera jamesonii Bolus ex Hooker f.)

anglais. Genetic diversity is the best way to improve available genetic resources for breeding programs in gerbera. In present study, genetic diversity of 22 gerbera cultivars was investigated using inter-simple sequence repeat (ISSR), directly amplified minisatellite DNA (DAMD), and Random Amplified Polymorphic DNA (RAPD) markers. Average polymorphism information content (PIC) for ISSR, DAMD and RAPD markers was 0.40, 0.41 and 0.40, respectively. Cluster analysis for ISSR, DAMD and RAPD divided the cultivars into three distinct clusters. The comparative analysis of the three markers (ISSR, DAMD and RAPD) showed that DAMD had superiority over RAPD and ISSR in characterization of genetic diversity in Gerbera. To our knowledge, this is the first report of a comparison of performance among DAMD, ISSR and RAPD techniques on a set of gerbera genotypes. Overall, our results showed that DAMD markers well represented different genotypes of gerbera diversity.

slovène. Genetska raznolikost je izhodišče razpoložljivih genetskih virov v žlahtniteljskih programih gerbere. V študiji smo genetsko raznolikost 22 sort gerbere preučevali z označevalci ISSR (inter-simple sequence repeat, regije med mikrosateliti), DAMD (direct amplified minisatellite DNA, pomnoženi minisateliti) in RAPD (Random Amplified Polymorphic DNA, naključno pomnožena polimorfna DNA). Popreprečna informacijska vrednost polimorfizma (PIC) je bila za ISSR, DAMD in RAPD markerje 0,40; 0,41 in 0,40. Klasterska analiza ISSR, DAMD in RAPD markerjev je sorte razdelila v tri različne skupine. Primerjalna analiza treh markerjev (ISSR, DAMD in RAPD) je pokazala, da so imajo DAMD markerji prednost pred RAPD in ISSR za določitev genetske raznolikosti pri gerberi. Pričujoča raziskava je ena prvih, ki primerja učinkovitost DAMD, ISSR in RAPD markerjev na izbranem naboru genotipov gerber. Naši rezultati so pokazali, da so markerji DAMD najbolje prikazali raznolikost genotipov gerbere.