Combined use of chlorophyll a and phycocyanin fluorescence sensors for quantification and differentiation of phytoplankton: a useful approach for small surface water bodies | Kombinirana uporaba klorofilnega in fikocianinskega senzorja fluorescence za kvantifikacijo in kvalifikacijo fitoplanktona: uporaba v majhnih vodnih telesih

anglais. Sensors based on in vivo measurements of photosynthetic pigments fluorescence enable real-time phytoplankton monitoring with high spatial and temporal resolution. A combination of chlorophyll a (CHL) and phycocyanin (PC) fluorescence sensors was used for phytoplankton quantification and differentiation in two small water bodies, Koseze Pond and pond in Hotinja vas. The high correlation of CHL and PC fluorescence signals with biovolume was confirmed during the two-year monitoring in anatural pond environment in spite of a seasonal succession of the phytoplankton. Additionally, disturbances of the sensors were investigated. Water bodies containing predominantly algae yielded false positive signals of the PC sensor, which reached up to 1% of the intensity of the CHL signal. Similarly, underestimated counts of cyanobacteria measured with CHL fluorescence sensor can be adjusted using PC fluorescence sensor.

slovène. Senzorji za merjenje in vivo fluorescence fotosintetskih pigmentov omogočajo meritve fitoplanktona z visoko časovno in prostorsko ločljivostjo. Kombinacijo klorofilnega (CHL) in fikocianinskega (PC) senzorja smo uporabili za kvantitativno in kvalitativno spremljanje fitoplanktona v dveh majhnih vodnih telesih, Koseškem bajerju in ribniku v Hotinji vasi. Kljub spreminjanju vrstne sestave je ostala korelacija med signalom CHL senzorja in biovolumnom visoka. Opredelili smo tudi napake senzorjev. V primeru vodnih teles z visoko koncentracijo zelenih alg je PC senzor dajal lažen pozitiven signal v velikosti 1 % signal CHL senzorja. S kombinacijo obeh senzorjev je mogoče odpraviti tudi podcenitev koncentracije cianobakterij, do katere pride ob uporabi le CHL senzorja.