Engineered Fungus Thermothelomyces thermophilus Producing Plant Storage Proteins
2022
Larissa Balabanova | Aleksandra Seitkalieva | Yulia Yugay | Tatiana Rusapetova | Lubov Slepchenko | Anna Podvolotskaya | Margarita Yatsunskaya | Elena Vasyutkina | Oksana Son | Liudmila Tekutyeva | Yury Shkryl
An efficient Agrobacterium-mediated genetic transformation based on the plant binary vector pPZP-RCS2 was carried out for the multiple heterologous protein production in filamentous fungus Thermothelomyces :thermophilus F-859 (formerly Myceliophthora :thermophila F-859). The engineered fungus Th. :thermophilus was able to produce plant storage proteins of Zea :mays (&alpha:-zein Z19) and Amaranthus :hypochondriacus (albumin A1) to enrich fungal biomass by valuable nutritional proteins and improved amino acid content. The mRNA levels of z19 and a1 genes were significantly dependent on their driving promoters: the promoter of tryptophan synthase (PtrpC) was more efficient to express a1, while the promoter of translation elongation factor (Ptef) provided much higher levels of z19 transcript abundance. In general, the total recombinant proteins and amino acid contents were higher in the Ptef-containing clones. This work describes a new strategy to improve mycoprotein nutritive value by overexpression of plant storage proteins.
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