Comparative Analysis of Quantitative Methods for Campylobacter spp. Quantification: ISO 10272-2:2017, Tempo® and Real-Time PCR in Refrigerated and Frozen Turkey Cuts
2024
Carlos Alberto Führ | Audecir Giombelli | Marisete Fochesatto Cerutti | Guiomar Pedro Bergmann | Liris Kindlein
New technologies for more effective microbiological assays are being adopted by the food industry to intervene more rapidly in its production chain. The aim of this study was to evaluate the alternative methods of TEMPO®: CAM and real-time PCR (rtPCR) Biotecon®: in comparison with the ISO 10272-2:2017 reference method for Campylobacter spp. quantification in turkey meat, aiming to validate a quick and easily replicable method in these meat matrices. A total of 416 samples were analyzed over a one-year period. The TEMPO®: methodology showed inadequate performance with a significant difference (p <: 0.05) compared with the reference methodology: therefore, its use was not recommended for turkey meat matrices. However, the performance of the rtPCR Biotecon®: methodology showed adequate performance with no significant difference (p >: 0.05), and its use was recommended in turkey meat matrices. The study was limited to exclusive research in turkey meat matrices, and expansion of the research into other matrices is recommended to verify whether the behavior of alternative methodologies is similar. The findings of this study illustrate the necessity for a thorough and comprehensive evaluation during the implementation of alternative methodologies that may potentially supplant conventional approaches.
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