Green Nanoparticle Synthesis in the Application of Non-Bacterial Mastitis in Cattle
2025
Michał Motrenko | Agata Lange | Aleksandra Kalińska | Marcin Gołębiewski | Małgorzata Kunowska-Slósarz | Barbara Nasiłowska | Joanna Czwartos | Wojciech Skrzeczanowski | Aleksandra Orzeszko-Rywka | Tomasz Jagielski | Anna Hotowy | Mateusz Wierzbicki | Sławomir Jaworski
This study explores the potential of silver nanoparticles (AgNPs) synthesized through an eco-friendly method using coffee extract to combat non-bacterial mastitis in dairy cattle. Mastitis, often caused by pathogens such as yeasts and algae like Prototheca spp., poses a challenge due to the limited efficacy of traditional antibiotics. This research utilized strains isolated from mastitis milk and assessed the nanoparticles&rsquo: physicochemical properties, antimicrobial efficacy, and impact on biofilm formation and microorganism invasion. AgNPs demonstrated a spherical shape with a mean hydrodynamic diameter of ~87 nm and moderate colloidal stability. Antimicrobial tests revealed significant growth inhibition of yeast and Prototheca spp., with minimal inhibitory concentrations (MICs) as low as 10 mg/L for certain strains. Biofilm formation was notably disrupted, and microorganism invasion in bioprinted gels was significantly reduced, indicating the broad-spectrum potential of AgNPs. The study highlights the nanoparticles&rsquo: ability to damage cell membranes and inhibit metabolic activities, presenting a promising alternative for managing infections resistant to conventional treatments. These findings suggest that green-synthesized AgNPs could play a pivotal role in developing sustainable solutions for mastitis treatment, particularly for pathogens with limited treatment options.
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