Engineering design of disposable bioreactor for cultivation of microbiological rodenticide on grain
2025
Bespalov, Igor | Dorovskykh, Viktor | Bulgakov, Volodymyr | Aboltins, Aivars | Rucins, Adolfs | Kaminskyi, Viktor
The equipment and technology were considered for the production of wet grain biopreparation of a bactericidal rodenticide, based on the bacteria Salmonella enteritidis var. A means of combating mouse-like rodents in crop production is Issatchenko. The object of the research is the technological process and equipment for the stage of cultivation of the preparation on grain, which is usually conducted in glass or metal containers of up to 3 litres. A new solution is proposed of designing a disposable bioreactor with replaceable plastic bags, which corresponds to global trends in the development of small-scale fermentation production. Using the method of an engineering design, fundamental technological and technical solutions have been developed for the process of cultivating bacteria on grain, which ensure an increase in productivity, improved quality of the product, and comfortable working conditions for the personnel. A design was worked out, models of disposable bioreactors for fermentation of bacteria on grain were manufactured and studied in a metal container with a replaceable polypropylene bag for 2 kg of bactericidal rodenticide. By means of the bioreactor all the technological operations are performed from sterilization to packaging of the finished products, the indicators of the technology and bactericidal rodenticide exceeding the standard values. A set of equipment has been identified at the grain stage for the production of 512 kg of bactericidal rodenticide per day.
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Editeur Latvia University of Life Sciences and Technologies
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