Hemijska karakterizacija i biološka aktivnost soka od grožđa i vina iz vinogorja Fruške gore

Wine is a part of the tradition and history of many countries in the world. After The Seven Countries Study, MONICA project of the World Health Organization and defining the French paradox, that have shown a clear link between wine and its cardioprotective properties, numerous studies focused on researching other healthful benefits of wine. Since there is very little information regarding wines from Fruška Gora, that are well known and have a long tradition, there is a need for their chemical and biochemical characterization. For the purpose of this doctoral dissertation, grape juices and wines were taken from the same vineyards in a three year period (a total amount of 56 samples), and commercially available wines from Fruška Gora consisting of one variety and a few blended wines were bought (a total amount of 91 samples). A detailed polyphenolic profile of all samples was determined using spectrophotometric, LC-MS-MS and LC-UV-vis methods. Assessed biological activities included antioxidant (using 6 assays), neuroprotective (inhibition of acetylcholinesterase) and anti-inflammatory activity (following a decrease in the production of inflammatory mediators of the cyclooxygenase and lipoxygenase pathways). The first aim of this thesis was to define the differences in the polyphenolic composition and biological activity between grape juices and wines, and the variations of these parameters during three years. Results have confirmed that the wines contain a higher amount of all tested phenolic compounds, compared to juices, with the exception of the flavonol group (kaemepferol 3-O-glucoside, quercetin 3-O-glucoside and hyperoside) that was more abundant in grape juices. Grape juices showed an equally good or even a better activity than corresponding wines in some of the biological assays which is very significant since the juice is alcohol free and can be consumed by everyone regardless the age limits. Comparing obtained results for the three year period, greater variations were noticed and explained by changes in the climatic factors and technological processes. The second aim was to characterize and compare wines from the region of Fruška Gora made from international, autochtonous and domestic varieties. Obtained results pointed out compounds epicatechin and a few phenolic acids such as syrngic, 2,5-dihydroxybenzoic, caffeic and p-coumaric acids as features of wines from Fruška Gora region, when compared to literature data concerning wines of different varieties and regions in France, Spain, Croatia, China, South Africa, Brazil and Argentina. Compounds that characterize wines of single varieties, as well as wines originating from different vineyards were specified. Considering biological activity, wines from Fruška Gora were in the same range as international wines. Varieties that characterize vineyards of Fruška Gora such as Frankovka, Probus, Portugizer, Župljanka, Sila and Petra showed a better activity compared to international varieties, which can contribute to their affirmation on the domestic and international wine industry. It was also shown that a higher wine price does not guarantee better health benefits and richer polyphenolic content. Results obtained in this dissertation present a significant contribution to scientific literature that lacks information on Fruška Gora wines and grape juices, providing information on the polyphenolic content and biological activity of grapevine products of this well known wine region.