cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province
2000
LI Fan | ZHOU Xue-ping | CHEN Hai-ru | LI De-bao
The cDNA of coat protein gene and its 3′ non-coding region of tobacco mosaic virus and coat protein gene of cucumber mosaic virus isolated from the tobacco in Yunnan province was synthesized by RT-PCR. The amplified cDNA fragments were then cloned and sequenced. The fragment of TMV-YN comprises 684bp (EMBL accession number: AJ239099), including 480bp of CP gene encoding 158 amino acids and a 204bp of 3' non-coding region. Comparison either the nucleotide sequence of CP gene or that of 3' non-coding region demonstrated that the TMV-YN is identical to the TMV-U1 strain and TMV common strain in Korea, with 100% homology. The CP gene of CMV-YNa consists of 657nt encoding 218 amino acid (EMBL accession number: AJ239098). The nucleotide sequence of CMV-YNa has 96%, 93% and 92% nucleotide identities with CMV-RB, CMV-117F and CMV-Y, respectively.
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