Soybean <i>GmSNF4</i> Confers Salt–Alkali Stress Tolerance in Transgenic Plants
2025
Nan Ye | Jia-Shen Bian | Bai-Hui Zhou | Ling-Tao Yong | Ting Yang | Nan Wang | Yuan-Yuan Dong | Wei-Can Liu | Fa-Wei Wang | Hai-Yan Lv | Xiao-Wei Li
In order to mitigate the reduction in soybean yield caused by soil salinization, a soybean gene, <i>GmSNF4</i>, which promotes plant tolerance to salt–alkali stress, was identified in this study. The STRING database was used to predict the interaction between GmSNF4 and GmPKS4. The <i>GmPKS4</i> gene was experimentally shown to be involved in salt–alkali stress tolerance. Firstly, the yeast two-hybrid technique and bimolecular fluorescence complementation (BiFC) technique were used to confirm the interaction between GmSNF4 and GmPKS4: the AMPK-CBM-CBS1 conserved domain was thereby determined to be the region of the GmSNF4 protein involved in the interaction. Secondly, the <i>GmSNF4</i> gene was induced by salt–alkali stress according to qRT-PCR analysis, and the GmSNF4 protein was localized in the nucleus and cytoplasm. Finally, analysis of <i>GmSNF4</i>’s role in resistance to salt–alkali stress in transgenic soybean plants showed that transgenic lines had better phenotypic, physiological, and stress-related gene expression than non-transgenic soybeans. Thus, <i>GmSNF4</i> may play a significant role in plant salt–alkali stress tolerance.
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