A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project
2024
Twyford, Alex D. | Beasley, Jordan | Barnes, Ian | Allen, Heather | Azzopardi, Freja | Bell, David | Blaxter, Mark L. | Broad, Gavin | Campos Domínguez, Lucía | Choonea, Darren | Crowley, Liam | Cuber, Piotr | Cunliffe, Michael | Dombrowski, Alexandra | Douglas, Brian | Forrest, Laura L. | Gaya, Ester | Greeves, Clementine | Griffin, Claire | Harley, Joanna | Hart, Michelle L. | Holland, Peter W. H. | Hollingsworth, Peter M. | Januszczak, Inez | Jones, Amanda | Kersey, Paul | Kilias, Estelle | Lawniczak, Mara K. N. | Lewis, Owen T. | Mian, Sahr | Minotto, Alice | Misra, Raju | Mulhair, Peter O. | Pereira da Conceicoa, Lyndall | Price, Ben W. | Salatino, Silvia | Shaw, Felix | Sivell, Olga | Sivess, Laura | Uhl, Rebekka | Woof, Kieran | Twyford, Alex D. [0000-0002-8746-6617] | Beasley, Jordan [0000-0003-2878-0080] | Barnes, Ian [0000-0001-8322-6918] | Azzopardi, Freja [0000-0003-2043-7536] | Bell, David [0000-0002-1059-8777] | Blaxter, Mark L. [0000-0001-5902-6641] | Broad, Gavin [0000-0001-7223-5333] | Campos Domínguez, Lucía [0000-0002-8998-3394] | Crowley, Liam [0000-0001-6380-0329] | Forrest, Laura L. [0000-0002-0235-9506] | Gaya, Ester [0000-0001-6404-4297] | Greeves, Clementine [0000-0001-7394-8546] | Holland, Peter W. H. [0000-0003-1533-9376] | Hollingsworth, Peter M. [0000-0003-0602-0654] | Januszczak, Inez [0000-0003-3857-9989] | Lawniczak, Mara K. N. [0000-0002-3006-2080] | Lewis, Owen T. [0000-0001-7935-6111] | Mian, Sahr [0000-0001-7828-0629] | Minotto, Alice [0000-0002-1670-1675] | Mulhair, Peter O. [0000-0003-3311-4883] | Pereira da Conceicoa, Lyndall [0000-0001-9192-3051] | Price, Ben W. [0000-0001-5497-4087] | Sivell, Olga [0000-0003-1255-0045] | Sivess, Laura [0000-0002-0482-6534] | Woof, Kieran [0000-0003-0579-106X]
Biodiversity genomics research requires reliable organismal identification, which can be difficult based on morphology alone. DNA-based identification using DNA barcoding can provide confirmation of species identity and resolve taxonomic issues but is rarely used in studies generating reference genomes. Here, we describe the development and implementation of DNA barcoding for the Darwin Tree of Life Project (DToL), which aims to sequence and assemble high quality reference genomes for all eukaryotic species in Britain and Ireland. We present a standardised framework for DNA barcode sequencing and data interpretation that is then adapted for diverse organismal groups. DNA barcoding data from over 12,000 DToL specimens has identified up to 20% of samples requiring additional verification, with 2% of seed plants and 3.5% of animal specimens subsequently having their names changed. We also make recommendations for future developments using new sequencing approaches and streamlined bioinformatic approaches.
Afficher plus [+] Moins [-]We thank all the partners and collaborators of the Darwin Tree of Life for their contributions and support, and all the taxonomic experts for providing their time to help with collections and sample identification.
Afficher plus [+] Moins [-]Peer reviewed
Afficher plus [+] Moins [-]Mots clés AGROVOC
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