The catabolism of branched-chain amino acids and tyrosine has a low contribution to the mitochondrial metabolism during senescence in Brassica napus L
2025
Aubert, Mathieu | Clouet, Vanessa | Guilbaud, Florian | Bouchereau, Alain | Dellero, Younès | Institut de Génétique, Environnement et Protection des Plantes (IGEPP) ; Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) | Plateforme de Profilage Métabolique et de Métabolomique (P2M2) ; MetaboHUB-Grand-Ouest ; MetaboHUB-MetaboHUB | MA was supported by a PhD grant from the Région Bretagne and the French Research Ministry (MESR, Ministère de l’Enseignement Supérieur et de la Recherche). This work was supported by the IB2019_FLUCOLSA and IB2021_TRICYCLE projects funded by the Plant breeding and biology department from the National Research Institute for Agriculture, Food and the Environment and by the ELECTRA project funded by the University of Rennes (Scientific challenges 2023 call). We thank the Metabolic Profiling and Metabolomic Platform (P2M2, https://p2m2.hub.inrae.fr/) for providing instrument devices to quantify the 13C enrichment of plant central metabolites. We acknowledge Solenne Berardocco for technical assistance during GC-MS analysis. We gratefully acknowledge financial support from the funders.
International audience
Afficher plus [+] Moins [-]anglais. Winter oilseed rape (WOSR) is characterized by a low nitrogen remobilization efficiency during developmental leaf senescence (DLS) compared with cereal crops. The catabolism of branched-chain amino acids (BCAAs) and tyrosine plays a significant role in the recycling of protein-bound nitrogen and the fuelling of mitochondrial respiration during stress-induced senescence. In this study, we elucidated the role of BCAAs and tyrosine catabolism during DLS in WOSR. To this end, we employed a multi-faceted approach, integrating transcriptional fingerprints, mitochondrial respiration measurements, and 13C-labelling experiments during both DLS and dark-induced senescence (DIS). In general, the transcriptional regulation of BCAA and tyrosine catabolism was weakly correlated with apparent catabolic fluxes during DLS and DIS in WOSR. The absolute quantification and 13C analysis of organic and amino acids by GC-MS showed that the catabolism of BCAAs and tyrosine had a comparable contribution to the functioning of the tricarboxylic acid (TCA) cycle during DLS and DIS in WOSR. Isotopologue analysis of TCA cycle intermediates provided evidence that BCAA and tyrosine catabolism contribute minimally to the functioning of the TCA cycle during senescence. The role of BCAA and tyrosine catabolism could be essentially devoted to the recycling of nitrogen during senescence.
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