Rumen microbiota and dietary effects on B vitamins in milk and blood
2025
Graulet, Benoit | Auzance, Lucie | Popova, Milka | Tournayre, Jérémy | Forano, Evelyne | Laverroux, Sophie | Martin, Bruno | Delbès, Céline | Verdier-Metz, Isabelle | Bouchon, Matthieu | Theil, Sébastien | Michalski, Marie-Caroline | Yuschenko, Alla | Duplessis, Mélissa | Ronholm, Jennifer | Dou, Paul | Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH) ; VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Microbiologie Environnement Digestif Santé (MEDIS) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA) | Unité Mixte de Recherche sur le Fromage (UMRF) ; VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA) | Unité Expérimentale Systèmes d'Elevage de Ruminants de Moyenne Montagne (Herbipôle) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RA) ; Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Centre Hospitalier Universitaire [CHU Grenoble] (CHUGA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA) | Métaprogrammes Syalsa (projet BestDairy) et Holoflux (projet Tandem) | International Research Network Galactinnov | INRAE-département Phase, crédit incitatif New approaches to evaluate the B vitamin status in the dairy cow | Université de Montréal | ANR-16-IDEX-0001,CAP 20-25,CAP 20-25(2016)
International audience
Afficher plus [+] Moins [-]anglais. B vitamins in milk can come from a cow’s diet but also from ruminal synthesis. Recent studies indicate that cows’ diet can have an impact on B vitamin content of milk but it is not known if the vitamin status of the cows is also impacted. This study investigated how the abundance of vitamin B1, B2, B5, B6, B9, and B12 in milk and of potential blood biomarkers for these vitamins vary, as well as the impact of ruminal synthesis. Four groups of 10 dairy cows, received diets differing in the percentage and nature of forage. Individual milk, blood, and ruminal contents were collected after 8 weeks (July sampling) and after 19 weeks (September sampling) on these diets. Samples were analyzed for vitamin content B vitamins. RNA sequencing as well as 16s rRNA gene sequencing were performed on stomach fluid samples from each cow to investigate the functional and taxonomic composition of the microbiota present and determine how this community contributes to vitamin production. Vitamin B2 content in milk was higher when the proportion of grass increased in the diet, but only in July. Vitamin B12 content is higher with a corn-based diet with a little access to pasture. Vitamin B5 content is the highest for corn-based diets without grazing time. Surprisingly, no variation was observed at the blood level. RNA sequencing results show that pathways of B vitamin synthesis are upregulated in the conditions that corresponded to increased B vitamin content. However, some of the variations of the gene activities were not related to variations of B vitamins concentrations in milk or blood. Several hypotheses could explain this. The compounds quantified in blood could be not good biomarkers of B vitamins. B vitamins can also be degraded in the rumen and use or degradation of vitamins in other organs may explain the disconnect between the concentrations in milk or blood. Future investigations of B vitamins production and use by rumen microbiota may help to shed light on the relationships between feed and milk B vitamin concentrations.
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