Effect of 6-Benzylaminopurine (6-BAP) on Shoot Proliferation in Micropropagated Cavendish Banana (Musa acuminata)
2025
Yanvary Chhon | Virak Sorn | Phanith Seab | Sivlin Ung | Laiheang Kheang | Viboth Ly
Plant development and growth are significantly influenced by plant hormones. 6-benzylaminopurine (6-BAP), a type of cytokinin hormone, is known to influence plant growth, development, and cell division. This study aims to determine the optimal concentration of 6-BAP for promoting shoot proliferation and growth under the tissue culture of Cavendish banana (Musa acuminata) in vitro. Suckers of 1 month old were used as explants, and two rounds of subculturing were conducted. The first subculture showed that banana explants treated with 3.0-mg/L 6-BAP produced an average of 4.17 shoots, significantly higher than other treatments under solid media conditions. The second subculture showed that the optimal concentration was 2.5 mg/L, yielding an average of 14.67 shoots per explant. For rooting, MS medium was supplemented with 1.0-mg/L indole-3-acetic acid (IAA), which significantly promoted rooting. The study demonstrates that 6-BAP significantly enhances shoot proliferation in Cavendish banana tissue culture, with the optimal concentration for initial shoot proliferation being 3.0 and 2.5 mg/L for multiple shoot generation. These findings hold significant implications for commercial banana propagation, as they can reduce the time and cost associated with plantlet production while ensuring uniformity and high multiplication rates, which are key factors in meeting the growing demand for disease-free and high-yielding planting material.
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