Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
2025
Ulises Alvarado | Jhon Tacuri | Alejandro Coloma | Edgar Gallegos Rojas | Herbert Callo | Cristina Valencia-Sullca | Nancy Curasi Rafael | Manuel Castillo
Monitoring pH and acidity during yoghurt fermentation is essential for product quality and process efficiency. Conventional measurement methods, however, are invasive and labour-intensive. This study developed artificial neural network (ANN) models to predict pH and titratable acidity during yoghurt fermentation using CIELAB colour parameters (L, a*, b*). Reconstituted milk powder with 12% total solids was prepared with varying protein levels (4.2–4.8%), inoculum concentrations (1–3%), and fermentation temperatures (36–44 °C). Data were collected every 10 min until pH 4.6 was reached. Forty models were trained for each output variable, using 90% of the data for training and 10% for validation. The first two phases of the fermentation process were clearly distinguishable, lasting between 4.5 and 7 h and exceeding 0.6% lactic acid in all treatments evaluated. The best pH model used two hidden layers with 28 neurons (R<sup>2</sup> = 0.969; RMSE = 0.007), while the optimal acidity model had four hidden layers with 32 neurons (R<sup>2</sup> = 0.868; RMSE = 0.002). The strong correlation between colour and physicochemical changes confirms the feasibility of this non-destructive approach. Integrating ANN models and colourimetry offers a practical solution for real-time monitoring, helping improve process control in industrial yoghurt production.
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