VP1 Mutation Affects Iron Distribution and Ferritin Accumulation in Maize (Zea mays) Seeds
2025
Schomburgk, Carlos | Urbina, Pia | Fuenzalida, Marlene | Navarro, Nathalia | Cancino, Felipe A. | González, Pablo A. | Martínez Barradas, Vera | Escudero, Viviana | Dubos, Christian | Roschzttardtz, Hannetz | Agencia Nacional de Investigación y Desarrollo (Chile) | Agence Nationale de la Recherche (France) | Dubos, Christian [0000-0001-5486-3643] | Roschzttardtz, Hannetz [0000-0002-2614-2504] | Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
The distribution of iron in maize (Zea mays L.) seeds is essential for both seed development and its nutritional value. In this study, we analyzed the role of the VIVIPAROUS1 (VP1) gene in regulating iron localization and ferritin accumulation using loss-of-function mutant alleles. Histological stainings revealed significant iron mislocalization in vp1 mutants, particularly in the scutellum, aleurone cells, and cells surrounding the provasculature, altering the iron distribution pattern observed in wild-type seeds but not the total iron content. Western blot analysis highlighted an increase in FERRITIN protein accumulation in mutant seeds, suggesting that VP1 plays a crucial role in repressing FERRITIN gene expression. Our findings highlight the genetic regulation of iron storage and transport by VP1, with implications for both seed development and nutritional improvement in maize crops.
Afficher plus [+] Moins [-]This work was supported by the Agencia Nacional de Investigación y Desarrollo (#1231048, #1240971); Agence Nationale de la Recherche (ANR-24-CE20-0000); ANID Iniciativa Científica Milenio—Millennium Institute on Immunology and Immunotherapy (ICN2021_045); ANID-ANILLO (ATE230007); ANID PhD fellow (#21251401).
Afficher plus [+] Moins [-]Peer reviewed
Afficher plus [+] Moins [-]Mots clés AGROVOC
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