Effect of planting material and spore preservation temperature of Astraeus odoratus on the growth and ectomycorrhizal root formation in Dipterocarpus alatus seedlings | ผลของวัสดุปลูกและอุณหภูมิในการเก็บรักษาสปอร์เห็ดเผาะหนัง (Astraeus odoratus) ต่อการเติบโตและการเกิดรากเอคโตไมคอร์ไรซาของกล้าไม้ยางนา (Dipterocarpus alatus)
2021
Thapana Chabthaisong(Kasetsart University, Bangkok (Thailand). Faculty of Forestry. Department of Forest Biology) | Tharnrat Kaewgrajang(Kasetsart University, Bangkok (Thailand). Faculty of Forestry. Department of Forest Biology)
The objectives of this study were to study the effect of planting materials and temperature on the preservation of Astraeus odoratus spores and their ability to grow and form ectomycorrhizal (ECM) root in Dipterocarpus alatus seedlings. Two formulae of planting materials included Formula 1 consisting of vermiculite+peat moss (8:2): and Formula 2 consisting of soil+granules black rice husk+coconut husk (1:1:1), were used in the experiment. The result indicated that seedlings inoculated with Formula 1 formed ECM colonization at a rate of 42.09 percent, which was not observed with Formula 2. Moreover, a relative growth rate of height was observed which was significantly higher than that in seedlings inoculated with Formula 2. To study the effect of temperature on preservation of A. odoratus spores, we preserved the spores for two years under three different temperatures including room temperature, 4 deg C, and -20 deg C. The result indicated that a 2-year-old preserved spore can be used as an ECM inoculum. The appropriate temperature for preservation spore was -20 deg C. In addition, the inoculated seedlings with spores preserved at -20 deg C showed a significantly higher ECM colonization rate (61.04 percent) than the inoculated seedlings with spores preserved at room temperature (22.51 percent)
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