Modulating Protein Glycation in Skim Milk Powder via Low Humidity Dry Heating to Improve Its Heat-Stabilizing Properties
2025
Zijun Zhao | Riza Flores | Bruno De Meulenaer | Paul Van der Meeren
The limited heat stability of skim milk powder (SMP) constrains its application in high-temperature processes. While dry heating can improve its thermal resistance, it often accelerates the advanced Maillard reaction, compromising protein quality. This study applied low relative humidity conditions (<:10% RH) during dry heating to modulate the Maillard reaction, aiming to enhance the heat resistance of SMP and derive recombined filled evaporated milk emulsions with fewer undesirable changes in colour and solubility. SMP was subjected to dry heating at 80, 100, and 120 °:C for durations ranging from 2 to 20 min (at 120 °:C) and up to 16 h (at 80 °:C). The progression of the Maillard reaction and associated protein modifications were evaluated. The results indicate that the advanced Maillard reaction was retarded, evidenced by minimal colour development and well-preserved protein solubility (90&ndash:97%, n = 3), determined using the Lowry assay on the supernatants. The hydroxymethylfurfural and protein carbonyl contents increased only moderately with temperature and time. Moreover, the sulfhydryl group content remained largely stable, consistent with limited disulfide-mediated aggregation. Heat treatment of SMP at 120 °:C for 10 min greatly improved its heat stability, as reflected by a 25-fold reduction in the volume-weighted average diameter (D4,3: 95% CI = 3 to 47) and a 108-fold reduction in the consistency coefficient (K: 95% CI = 12 to 200) of the SMP-derived sterilised recombined filled evaporated milk (RFEM) compared to the control. These findings demonstrate that dry heating under low RH helps to improve the functional properties of SMP without inducing the detrimental effects associated with advanced Maillard products.
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