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Factors affecting total antioxidant activity and polyphenol content in beet root chips during production and storage Texte intégral
2022
V. A. Zayachkovsky | A. I. Moldovan | V. I. Tereshonok | V. A. Kharchenko | M. S. Antoshkina | L. V. Pavlov | N. A. Golubkina | V. A. Stepanov
Relevance and methods. Chips are considered to be one of the most popular food products with high content of biologically active compounds. The aim of the present work was evaluation of quality and antioxidant self-life of beet chips from different cultivars. Effect of drying method (convection, freeze drying) and storage conditions (paper, vacuum plastic bags and aluminum foil) on beet root chips from 4 cvs self-life was investigated. Biochemical methods of analysis and tasting evaluation were used.Results. Beet intervarietal differences in self-life of chips fat soluble antioxidants were indicated. Paper bags were shown to produce the highest AOA and phenolics values of convection drying products contrary to freeze drying chips in vacuum plastic bags. Differences in AOA and phenolics self-life of products produced via convection and freeze drying are absent. Tasting evaluation (appearance, taste, texture, aroma) revealed the lowest score for Gaspadynya cv. The highest polyphenols loss in chips stored in aluminum foil was typical for Gaspadynya cv chips, while storage in paper bags – for chips from Dobrynya cv chips. In a whole, the results of the present investigation indicate the importance intervarietal differences in beet chips production, as genetic differences determine taste and storability of the product.
Afficher plus [+] Moins [-]Phenolic compounds of cultivated chicory (<i>Cichorium intybus</i> L.) Texte intégral
2022
P. O. Mavrina | E. L. Malankina
Common chicory (Cichorium intybus L.) is widely known in traditional medicine of many countries, but in official medicine its use is limited as a part of biologically active additives. Despite the available information about pharmacological activity of leaf extracts, their use as a medicinal herbal raw material is almost not considered. The purpose of the work is to study the accumulation dynamics of phenolic compounds (PC) in the leaves of cultivated chicory to optimize the time of harvesting the above-ground mass. The article presents data on the accumulation dynamics of PC in the leaves of two varieties of cultivated chicory, and also compares the obtained data with similars of wild-growing chicory.Results. It was noted that the content of phenolic compounds in the leaves of cultivated chicory is 1,5-2 times higher in comparison with the raw materials of wild plants. The studied varieties differ in the content of PC by 8% in average. It has been established that the main phenolic compounds in the leaves of cultivated chicory are chicoric, chlorogenic and cafftaric acids; their total content in the obtained extracts is 55 %. It was noted that the period of maximum accumulation of PC in leaves falls on the end of september – beginning of october. The established chemical composition, a higher content of phenolic compounds compared to wild plants, as well as the coincidence of the maximum accumulation of PC in the leaves with the approximate date of harvesting chicory roots allows us to consider its cultivation in culture as a promising direction with the aim of the integrated use of the obtained raw materials for both food and pharmaceutical industry.
Afficher plus [+] Moins [-]Growth of internodes and branching of a tomato plant Texte intégral
2022
V. G. Korol
Relevance. A significant influence on the use of the volume of greenhouses by a tomato plant is its height, which consists of the length of the internodes of monopodial and sympodial shoots. The use of the volume of greenhouses in the cultivation of tomato crops is a relevant and important topic. Currently, tomatoes are grown in greenhouses of various heights and designs. Greenhouses of different sizes, heights and types of cover are used in a farmer's market. And here the height of tomato plants is of decisive importance. Mostly indeterminate plants are grown, limiting their growth to a certain number of inflorescences, less often determinant plants. When grown in modern industrial greenhouses and the vegetation period is 1012 months a year, preference is given to tall indeterminate tomato hybrids that have almost continuous growth due to the formation of more and more sympodial shoots and high yield potential.Methods. The studies were carried out in a winter glazed greenhouse. The tomato culture was grown in an extended rotation (from January to November) on a coconut substrate with drip irrigation. Tomato hybrids of domestic and foreign selection with determinant and indeterminate types of growth were grown. Phytocenosis density is 2.8 plants/m2, from April to September it thickened up to 3.4 plants/m2 due to the formation of additional shoots. All observations were carried out according to generally accepted methods. Results. The variety grown in protected ground depends on the growing conditions. A pattern was established for the length of the internodes of sympodial shoots in indeterminate tomato hybrids, the third internode is longer than the first two. The correlation coefficient between the length of the sympodial shoot and the length of the third internode is 0.85 points. The enhanced growth of the last internode is caused by the growth of the shoot of the next order. In this case, the removal of the leaf above the inflorescence is 2-5 cm. The correlation coefficient between the length of the third internode and the removal of the leaf above the inflorescence is 0.71 points. A pattern was established for the length of the leaves and the development of stepchildren in the sympodial shoot.
Afficher plus [+] Moins [-]Efficiency of joint glycine and auxin analogs foliar treatment on coriander seeds yield and essential oil quality Texte intégral
2022
P. V. Pochuev | N. G. Romanova | E. L. Malankina
Coriander is the leading essential oil crop and occupies vast areas, both in our country and abroad, and is be used to produce linalool, with subsequent processing into other aromatic substances. Increasing its yield and quality of raw materials with the help of modern preparations is an urgent problem of essential oil production.Purpose of the work: increasing the productivity of coriander with combined use of foliar treatments with glycine and auxin-containing preparations for directed control production process in coriander seed.Material and methods. As objects for studying the effect of the drug, varieties of coriander sowing Yantar and Avangard were chosen. Plants were sown in the first or third decade of April, depending on the conditions of the year, using a SZT-3.6 seeder with row spacing of 15 cm. The seeding rate was 25 kg/ha, the seeding depth was 2 cm. concentration 10 mg/l. Drug concentrations: IAA-glycol phosphate – 25, 50 and 100 mg/l, DvaU - 2 ml/l. Treatment with a solution of IUKGF and DvaU was carried out in the phase of budding-beginning of flowering. The cutting was carried out during the period of browning of seeds on the central umbrella. The content of essential oil was determined according to SP XIV method 1.Results. As a result of the research, a positive effect of foliar treatments with glycine was revealed, both on the yield and on the content of essential oil in the raw material of coriander seed varieties Yantar and Avangard. Based on the results obtained, the effective concentration of the drug is determined not only by the characteristics of the variety, but also by weather conditions, when, depending on the conditions during the processing period and the previous harvest, different aspects of the drug action appear. The maximum increase in the collection of essential oil of coriander variety Yantar (11.1 kg/ha) was noted during the combined treatment of plants with glycine at a rate of 10 mg/l in the leaf rosette phase and IAA-GF at a rate of 50 mg/l in the budding phase; varieties Avangard (8.6 kg/ha) - with combined treatment of plants with glycine at a rate of 10 mg/l in the leaf rosette phase and DvaU at a rate of 2 ml/l in the budding phase.
Afficher plus [+] Moins [-]Effect of sodium selenate foliar supplementation on Cryptotaenia japonica and Petroselinum crispum nutritional characteristics and seed quality Texte intégral
2022
V. A. Kharchenko | A. I. Moldovan | Z. A. Amagova | V. Kh. Matsadze | N. A. Golubkina | G. Caruso
Production of functional food with high levels of selenium and other antioxidants is very valuable for human protection against different forms of oxidant stress. Among leafy vegetables parsley demonstrate the highest levels of antioxidants. Biochemical analysis and fluorimetric determination of selenium revealed that foliar biofortification of 4 parsley (Petroselinum crispum) cultivars and Mitsuba (Cryptotaenia japonica) with sodium selenate (25 mg L-1) resulted in the highest biofortification level in curly parsley cultivar Krasotka (102.9) which showed the highest leaf surface area, antioxidant activity (65 mg GAE g-1 d.w.) and flavonoids content (25.9 mg quercetin equivalent g-1 d.w.), and the increase by 1.4 times in carotene content and 1,5 times in total chlorophyll content. ICP-MS method of mineral composition evaluation recorded extremely high levels of B and Si in Mustuba, which increased due to Se supplementation by 1.23 and 1.46 times respectively. In a two-year experiment with control and Se-fortified, leafy parsley, cultivar Moskvichka reached high values of seed yield and viability, and seed Se content (6170 µg kg-1 d.w.). The results of the present investigation demonstrate high prospects of parsley and Mitsuba selenium biofortification for production of functional food with elevated levels of microelement and high antioxidant activity.
Afficher plus [+] Moins [-]Evaluation of intravarietal polymorphism of tomato fungal disease resistance genes in Michurinsky State Agrarian University breeding varieties Texte intégral
2022
I. N. Shamshin | A. S. Ilyichev | E. V. Grosheva
Relevance. The studies are aimed at assessing the intravarietal allelic diversity of genes for resistance to fusariosis and cladosporiosis of tomato cultivars bred at the Michurinsky State Agrarian University using molecular markers.Methods. The biological objects of the study are tomato varieties bred at the Michurinsky State Agrarian University. A total of 10 varieties were analyzed. The assessment of intravarietal polymorphism was determined on a sample of 10 plants of each variety. DNA extraction was performed using a Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, USA) for DNA extraction according to the manufacturer's protocol. The P7 DNA marker was used to identify the cladosporosis resistance gene Cf-19. The presence of the fusarium wilt resistance gene I-2 was determined using the marker I-2/5. Amplification results were visualized by electrophoresis in 2% agarose gel.Results. 10 plants of 10 varieties of Michurinsky GAU selection tomato were analyzed using molecular markers of genes for resistance to cladosporiosis and fusariosis. An assessment of intravarietal polymorphism was carried out. In most of the varieties studied, the genes analyzed are identified in a heterozygous state. Three varieties (Vivat, Carotinka, Krasavets) containing only alleles of susceptibility to the causative agent of the disease in the genotype I-2 identified by the gene. Individual samples were homozygous for the fusariosis resistance allele. Analysis of the gene for Cf-19 resistance to cladosporiosis showed that most of the varieties studied are heterozygous forms. In some varieties, all ten plants were heterozygous. These include Krasavets, Vivat, Orlik, Bui Tour. The remaining samples had different allelic compositions. The pathogen resistance allele has been identified in plants of the Nepryadva and Chernysh varieties. The dominant allele in the homozygous state was detected in the Japanese variety. At the same time, all the analyzed plants of this variety were monotonous.Conclusion. It has been established, but a significant part of the varieties of tomato selection of Michurinsky GAU is polymorphic in the genes for resistance to cladosporiosis and fusariosis. In this case, a significant part of the varieties can be used in selection during preliminary molecular analysis. According to the gene for resistance to fusariosis, the following varieties are identified sources of the resistance allele: Sokol, Nepryadva, Yaponchik, Orlik, Zolotnichok, Chernysh. In them, the gene is I-2 represented in a heterozygous state. Intraortic polymorphism was also noted for the Cf-19 gene. Most samples have two alleles. The exception is the Japanese variety, which is the dominant homozygote for this gene.
Afficher plus [+] Moins [-]Breeding of parthenocarpic cucumber hybrids for unheated greenhouses Texte intégral
2022
L. A. Chistjakova | O. V. Baklanova
Relevance. Cucumber is very popular and in demand in households of the population (personal subsidiary farms), as evidenced by the data on the acreage occupied under this crop in the territory of the Russian Federation (37.4 thousand hectares).Methodology. This article presents the results of evaluating the combinational ability of eight parthenocarpic inbreeding lines and testing thirteen new hybrid combinations obtained on the basis of the studied lines under the conditions of spring-summer turnover in a polycarbonate unheated greenhouse in the Moscow region in comparison with the hybrids F1 SV 4097 CV (Seminis, the Netherlands) and F1 Piligrim (Agrofirma Poisk LLC, Russia).The purpose of the scientific work is to develop productive and high-yielding heterotic cucumber hybrids with a complex of economically useful traits for cultivation in unheated greenhouses. Testing and introduction of competitive and technologically advanced hybrids into commercial vegetable production is one of the important stages of scientific research in the field of breeding. The research was carried out in ARRIVG – a branch of FSBSI “Federal Scientific Vegeculture Center” in the conditions of polycarbonate unheated ground greenhouses during 2020-2021 (Moscow region, Ramensky district).Results. As a result of evaluation of the combinational ability of parthenocarpic inbreeding lines, it was found that inbreeding lines L.21 (2.4 kg/m2 ), L.415 (0.8 kg/m2 ), L.993 (0.4 kg/m2 ), L.1010 (0.1 kg/m2 ) should be used during hybridization as a paternal component for obtaining early-ripening parthenocarpic cucumber hybrids. To develop more productive and high-yielding parthenocarpic cucumber hybrids, inbreeding lines L.21, L.415, L.1008 should be used. As a result of the research work, four hybrid combinations (L.993×L.415, L.1010×L.21, L.993×L.21, L.993×L.1008) were identified for high yield and productivity in protected soil conditions, which exceeded the standards of SV 4097 CV F1 and Piligrim F1.
Afficher plus [+] Moins [-]Foliar biofortification of chervil with selenium and iodine under silicon containing fertilizer supply Texte intégral
2022
A. I. Moldovan | V. A. Kharchenko | N. A. Golubkina | E. D. Kekina | G. Caruso
Selenium (Se) and iodine (I) are essential elements for humans, and their deficiency is widespread throughout the world. In order to obtain a functional nutritional product with an increased content of these trace elements in the vegetative experiment, foliar biofortification of two chervil varieties with selenium (sodium selenate 10 mg/l) and iodine (potassium iodide 100 mg/l) was carried out without and against the background of the use of silicon-containing fertilizers Siliplant (3 ml/l). The combined and separate application of selenate, iodide and Siliplant increased plants’ biomass. Siliplant utilization increased the accumulation of iodine by 1.7-1.9 times, and selenium supply – by 2.2-3.1 times. A significant increase in ascorbic acid content was provided by the combined supplementation of iodine and selenium (1.25-1.27 times), iodine and silicon (1.46-1.87 times) and joint application of selenium, iodine, and silicon (1.31-1.73 times), while an increase in total antioxidant activity (1.3-1.4 times) was observed for (Se+I) and (Se+I+Si) treatments. High varietal differences in the responsiveness of plants to the selected treatments were manifested, particularly an increase of polyphenols accumulation under separate and joint treatments of chervil with iodine and selenium by 1.26 times in the cultivar 21-20, and the absence of a significant effect in the cultivar 24-20. Taking into account the adequate consumption levels (ACL) of iodine and selenium, 50 g of the resulting functional product can provide up to 79% of iodine ACL and up to 40% in selenium ACL.
Afficher plus [+] Moins [-]The effect of mulching materials on yield, biochemical composition and preservation of fruits of bell pepper Texte intégral
2022
E. V. Yanchenko | D. I. Engalychev | N. A. Engalycheva | K. L. Alekseeva
Introduction. The use of new covering materials is an important modern agrotechnique for cultivating of bell pepper in the open ground, which allows to minimize chemical treatments, increasing the yield, quality and preservation quality of vegetable products.The purpose of this work is to study the effect of various mulching materials (black polyethylene film and spandbond «Agrotex 60 perforated mulch») on the yield of bell pepper and also on biochemical composition of fruits and on their preservation quality in relation to the ripeness phase.Methods. The objects of the study were the fruits of two bell pepper hybrids of domestic and foreign breeding (respectively Nathalie F1 and Anetta F1), grown in the open ground using mulching materials. The experiments were laid according to standard methods adopted in vegetable growing. Storage was carried out in accordance with the methodological guidelines for conducting research on the storage of vegetables.Results. The use of mulching materials contributed to an increase in total yield and the yield of standard products in the crop structure, and also positively affected the accumulation of solids, vitamin C, monosaccharides, disaccharides and as a whole – the sum of sugars. The domestic hybrid Natalie F1 (74.9%), grown using black film as a mulching material, as compared to foreign hybrid Anetta F1 (71.3%), was characterized by better preservation quality. Significant differences in the chemical composition of fruits depending on the ripeness phase were revealed. Fruits laid for storage in full biological ripeness (red) were characterized by an increased amount of weight loss on all storage options. A positive effect on the storage persistence of bell pepper fruits with use of mulching materials on all variants of the experiment was also established.
Afficher plus [+] Moins [-]Creation of parthenocarpic lines of smooth and short type of cucumber Texte intégral
2022
N. N. Khomchenko | V. N. Shevkunov | V. N. Muliar | I. S. Pluzhnik | A. V. Kurepin
Relevance. Obtaining modern cucumber hybrids is impossible without the constant involvement of genetically new sources in the breeding process. One of the most important tasks in the issue of creating new heterotic cucumber hybrids is to obtain, and in the future, selection for hybrid crossings, parental lines with the most important economically valuable traits. Research is aimed at obtaining new short-fruited cucumber lines with a smooth type of fruit in order to obtain hybrids with their help that combine resistance to powdery mildew for the “pm” and “pmh” genes, tolerance to the zucchini yellow mosaic virus for the “zymv” genes in combined with high yields, the possibility of growing them not only in unheated film greenhouses, but also in glazed greenhouses.Methods. This article presents two schemes for obtaining parental forms using the example of creating four cucumber lines with a short, smooth fruit. Their comparative characteristics and description of new hybrids created with the participation of the studied linear material are carried out. Materials and methods. The main studies were carried out in 2013-2020 on the basis of the Pumpkin Cultures Laboratory of the Krymsk Breeding Center "Gavrish" of the Research Institute of Vegetable Breeding (Krymsk, Krasnodar Territory). The research material is F1 hybrids and selection samples of parthenocarpic cucumber with a smooth type of fruit of various origins (34 samples in total) from the NIISOK collection. The work used traditional methods for assessing selection-significant traits and modern methods of molecular labeling. The purpose of the work is to compare two schemes for obtaining short-fruited cucumber forms, to evaluate the created lines and hybrid combinations with their participation in terms of a complex of economically valuable traits.Results. The result of the work was the creation of new hybrid combinations based on the obtained lines, two of which are included in the State Register of Breeding Achievements. These are F1 Ministar (F1 1600/16) and F1 Promini (F1 1995/16).
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