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Antioxidant activity of potatoes (Solanum tuberosum L.) and anthocyanin content, its biosynthesis and physiological role
2019
O. B. Polivanova | E. M. Gins
Relevance. Potatoes with red and purple flesh are highly valued dietary products and sources of polyphenolic compounds with antioxidant activity, especially anthocyanins. It has been found that the anthocyanin content in potatoes correlates with the total soluble phenolic content and antioxidant activity. These measures are significantly higher for coloured potatoes. The total phenolic content in particular the total anthocyanin content increases during the cold storage of coloured potatoes. Pigmented potatoes are also more preferable for food and industrial processing because of retaining of antioxidant properties and improving of final products quality. Сoloured potatoes selection and biotechnological approaches of regulation of anthocyanins accumulation are based on the understanding of the molecular genetic processes of anthocyanins biosynthesis. The anthocyanin biosynthesis is a part of the phenylpropanoid metabolic pathway. It is controlled by the MBW complex. The MBW complex includes the transcription factors MYB, bHLH, and WD40. A number of the MYB complex genes have been identified in potatoes. The key role in potatoes pigmentation variability is currently assigned to the StAN1 gene. According to available data anthocyanins play a protective role in response to various types of stress in potatoes.
Afficher plus [+] Moins [-]INFLUENCE OF GENUS BACTERIA <i>BACILLUS</i> ON QUALITY OF <i>Lactuca sativa L.</i>
2019
O. V. Doroshchuk | J. N. Kalatskaja | N. A. Laman | V. V. Minkova | M. N. Mandrik-Litvinkovich
Primal problem of vegetable growing is constant supply of the population with all types of vegetables, including green cultures. Green cultures are vegetables that have high nutritional value and precocity. However they often are infected by phytopathogenic microorganisms already at initial stages of ontogenesis at cultivation in closed soil conditions. It leads to emergence of disjointed shoots, deterioration of growth and development of plants and loss of quality. Now in the Republic of Belarus a number of biological substances on the basis of bacteria Bacillus was developed. They are used against diseases of plants of mushroom and bacterial etiology. However there is not information about influence of bacteria on quality of products of green cultures. The aim of the work was studying of influence of two strains of bacteria Bacillus that were introduced in peat substrate on efficiency and quality of lettuce. Two strains of bacteria Bacillus were used in the work. They were selected from the soil. The strains are Bacillus subtilis M9/6 and Bacillus amyloliquefaciens 23TM that have high antagonistic activity to a wide range of phytopathogens. Cultivation of plants carried out in containers of 250 ml under light installations with illuminating intensity 13-15 thousand luxury and lasting irradiating of 14 hours before technical ripeness of lettuce. It was established that the application of strain Bacillus subtilis M9/6 (in concentration 106 cells/ml, 10 ml/l of substrate) and the strain Bacillus amyloliquefaciens 23TM (5 ml/l of substrate) in substrate before sowing increased nutrition value of lettuce. Dry matter content, water-soluble carbohydrates (mono - and disaccharides) content and vitamin C content increased. The bacterial strain B. amyloliquefaciens 23TM also promoted accumulation of vitamin B2. The content of nitrates in lettuce leaves decreased on 50,3% and 39,1%, respectively. It was shown that the application of bacteria in substrate before sowing of crop has a greater influence on quality of lettuce, than watering of shoots.
Afficher plus [+] Moins [-]SELECTION OF PARENTAL COMPONENTS OF SWEET PEPPER HYBRIDS FOR STEPPE AND DRY STEPPE
2019
E. A. Kozlovskaya | O. N. Pyshnaya | M. I. Mamedov | E. A. Dzhos | A. A. Matyukina
The main direction in the selection of sweet peppers is the creation of hybrids with a high heterosis effect. The question of components choice for the greatest effect in crossings is studied by breeders and geneticists of many countries for a long time. However, until now it has not been possible to create a theory that allows breeders conducting practical work to consciously and reliably choose pairs that would provide maxi-mum and stable yield and product quality, depending on the purpose of the variety. In the work various principles of the selection of pairs were used: ecological-geographical, according to the components of attributes and selection of the maternal form. Using these methods, more than 300 combinations of crosses were obtained. It is shown that the combination of two ecologically different parental components of different origin - the local sample and the introduced one - leads to an increase in the heterosis effect. The best hybrid combinations with a complex of economically valuable traits are obtained by crossing local varieties with selected breeding lines. When selecting pairs according to the components of the signs, where local or introduced samples with a different set of features were taken as parental forms, productivity was at the level of the standard. In most cases, when used as the maternal form of the local variety, the productivity of the hybrids was significantly higher compared to the reverse crossing. As a result of our studies, it is once again confirmed and proved that the more differences in the places of cultivation and reproduction of parental components, the higher the heterosis effect and the effect of the maternal component is much higher.
Afficher plus [+] Moins [-]STRUCTURE OF SOIL COMPLEXES OF MICROSCOPIC FUNGI UNDER DIFFERENT VARIETIES OF BROAD BEANS
2019
Yu. N. Kurkina
Considering of broad beans as an integral component of biological crop rotations, the presence of phytopathogenic, toxigenic, opportunistic, and allergenic microscopic fungi in soils under their different varieties should be examined. At the Botanical garden BelSU (Belgorod) on a natural infectious background were grown and studied of 16 different broad beans varieties. Were taking into account the indicators of frequency of occurrence and abundance of species, they determined the structures of soil mycocomplexes under different varieties. Analysis of the data showed that most micromycetes in the rhizosphere under bean varieties belonged to the Ascomycota division, which is part of it as 4th classes of Sordariomycetes, Dothideomycetes, Eurotiomycetes and Saccharomycetes. The species diversity of the soil mycobiota of steaming soil was characterized by 26 species, and under the studied bean varieties 57 species of fungi were identified. In the mycocomplex under varieties, species of different ranks of frequency of occurrence developed that were not noted in the paring soil: Alternaria tenuissima and Stemphyllium solani were rarely and accidentally found in the control soil, but they were not found in under the beans during budding – flowering. This can be explained by the fact that a different composition of exudates in the root zone of the soil under the plants contributes to the development of different types of microorganisms. In the complex of micromycetes under the beans, the share of toxigenic species increased by 2.5-29%, opportunistic species - by 1.3-31%, allergens - by 2-24% compared with the soaring soil. In the soil, an abundance of opportunistic and allergenic species capable of inducing mycoses and allergies in humans can be reduced by the broad bean varieties Aquadul, White large-fruited, Russian Black, Velena, Summer Resident, Leader, Optics, Pink Flamingo, Yankel Byala. After the cultivation of the beans the phytopathogenic species Ascochyta fabae, Clasdosporium herbarum, Fusarium graminearum, F. oxysporum var. orthoceras, F. oxysporum, F. solani and Ulocladium botrytis, remain in the soil and this fact must be considered when selecting the next crop in crop rotation.
Afficher plus [+] Moins [-]LARGE-FRUITED, DELICACY AND HIGHLY PALATABLE TOMATO VARIETIES
2019
I. Yu. Kondratyeva | M. R. Engalychev
The conflict has arisen between the production of “technical”, low quality tomato varieties in the interests of farmers and processers and the need of population accustomed to consumption of salad tomatoes in high quality, useful products. As far as now this conflict has not been settled. Tomatoes are among the most important year-round sources of vitamins С, В, В2, РР, А, Н, В9, pectines, valuable minerals and nutrients and also other compounds, especially the antioxidants lycopene and carotene, which determine healthy diet of humans and protect from many diseases. It is necessary to create the tomato varieties for each soil-climatic zone, which are adapted to specific environmental conditions of cultivation, thus maximizing the genetic and climatic potential of the crop. In non-chernozem zone of Russia, which is characterized by perhumidity but rather short vegetation period and insufficient sum of temperatures for such warm-weather crop as tomato, the plant breeders of Federal State-Financed research Institution, Federal Research Center of Vegetable Production “VNIISSOK” create primarily short-season and mi-season varieties, which are tolerant to the low temperatures, short-growth (tree-type and determinant bushes) with closely placed clusters, even ripening and resistant to shot holes.
Afficher plus [+] Moins [-]PRODUCTIVITY OF THE GREENHOUSE TOMATO CULTURE IN THE LONG TURNOVER IN THE NORTH
2018
T. К. Golovko | I. V. Dalke | G. N. Tabalenkova | R. V. Malyshev | E. E. Grygoray
The data on the greenhouse tomato culture productivity in the long turnover (February November) without application of artificial light are presented. The plants were grown using small-volume hydroponic technology on mineral wool in the industrial greenhouse of LLC "Prigorodny" (Syktyvkar, 61° 40′ 35″ N, 50° 48′ 35″ E). Tomato seeds (hybrid Starbuck F1) were sown in early December 2016. The seedlings were illuminated for 19 h daily with the high-pressure sodiumvapor reflector lamps (HPSV-400W / REFLUX) with an installed power of 130 W/m2. In early February 2017 plants at the age of 4245 days were transferred to the greenhouse and grown at density of 2 plants per square meter under natural lighting conditions. The dependence between the light intensity at leaf level and the natural light entering the greenhouse was studied. In spring sunny days, the light intensity at the level of the upper leaves did not exceed the 450500 μmol quanta/m2s, and in cloudy weather was 2-3 times lower. It was found that the saturation of leaf CO2-uptake by light took place at intensity of 800-1000 μmol quanta/m2s. The leaves formed under light deficiency had lowered photosynthetic activity (near 3 μmol СО2/m2s). The maximal productivity of tomato culture was observed during maximum light levels and sunny period (June-July). The average value of tomato productivity was about 34 kg/m2. The data show the possibility of producing commercial tomato yield in the first light zone without artificial lighting, which significantly reduces production costs.
Afficher plus [+] Moins [-]MANAGEMENT OF INNOVATIVE DEVELOPMENT OF VEGETABLE SUBCOMPLEX AGRARIAN SECTOR OF THE REGIONAL ECONOMY
2018
O. V. Mamai | N. N. Lipatova | M. N. Kupryaeva
The article reveals the essence and systemizes the advantages of agricultural cooperation. In the vegetable subcomplex of the agricultural sector of the economy, this type of cooperation helps attract investment and reduce risks for investors, increases the competitiveness and economic growth of the region. The relevance of the study is due to the fact that many aspects of the functioning of agricultural cooperatives remain inadequate to apply to individual sectors, in particular, to the vegetable subcomplex of the agricultural sector. The purpose of the research is to develop recommendations for managing the innovative development of the vegetable subcomplex of the agricultural sector of the regional economy (by the example of the Samara region). In preparing the article, general scientific methods of research were used: analysis and synthesis, generalization, comparison, classification. The state of vegetable growing in the Samara region is analyzed. The main problems of its innovative development are singled out. The advantages of agricultural cooperation as a modern tool for management of innovative development are systematized. The importance of cooperation within the vegetable subcomplex of the agrarian sector of the regional economy, which is of strategic importance for ensuring the food security of the region and Russia in general. Existing models of cooperation in vegetable growing in the Samara region are analyzed. The goal, tasks and main problems of the management system of innovative development of the vegetable subcomplex of the agrarian sector of the regional economy are formulated. As a result of the study, recommendations were proposed on the formation of a management system for innovative development of the vegetable subcomplex of the agrarian sector of the regional economy.
Afficher plus [+] Moins [-]DNA MARKERS IN ONION (Allium cepa L.) CYTOPLASMIC MALE STERILITY STUDY
2018
I. V. Pavlova | N. P. Kupreenko | A. S. Bulahova
The work is carried out in the framework of the onion F1 breeding program. The practical purpose of the work is to carry out genetic selection of onion forms using molecular genetic markers of male sterility in the course of obtaining the maternal line for heterotic selection of F1 hybrids. The scientific component of these studies was the study of the population-genetic basis of various types of male sterility in onions of the Belarusian genmplasma. We used belarusian varieties Vetraz and Skarb litvinov and a collection of other varieties and hybrids. Markers of the mitochondrial genes orfA501, cob and the nuclear alleles Ms/ms, cosegregating with genes of fertility restorer/S-sterility maintainer genes, were studied. It has been shown that in the Vetraz variety are observed plants with the N- or T-cytotype in N-cytoplasm (TN-). The belarusian variety Scarb litvinov contains plants with the cytotypes N-, S, TN-, SN-, STN-. As a result, it was concluded that the male sterility of the Vetraz variety is T-type, and in the Scarp litvinov variety is complex, and is caused by the cytoplasmic alleles S- and T-. In both varieties: Vetraz and Scarb litvinov the sources of the ms locus causing the S-type of male sterility have been identified. Less than one-fifth of the number of world collection of varieties and hybrids was detected as Scytotype. This value is smaller than the value given in the scientific literature. However it may reflect the significant spread of the original S-cytoplasm of a single plant of the shortday Italian Red variety in the world onion germplasm.
Afficher plus [+] Moins [-]HETEROSIS BREEDING OF ONION
2018
A. F. Agafonov | V. V. Logunova
One of the main directions of onion crops breeding in the world is heterosis breeding. Heterosis hybrids occupy the main place in the commodity production of onions in the Netherlands, USA, Japan and other countries. In State Register for Selection Achievements Admitted for Usage (National List) of the Russian Federation, admitted to use in 2018, made 378 cultivar of onions, including 188 (49.7%) – F1 hybrids, of which 140 – foreign breeding. A common way to create heterosis hybrids F1 onions is the use of lines with cytoplasmic male sterility (CMS) as one of the parent forms. The article presents the results of multi-year research of the laboratory of breeding and seed production of onion cultures (VNIISSOK, now FSBSI FSVC) to assess and highlight the lines of CMS onions with high combinational ability, the creation of heterosis hybrids on their basis. In 2002-2005, 229 cultivar of onion were studied to identify plants with pollen sterility and maintainer. 515 plants with CMS were isolated. In the subsequent years (2006-2010) was established 1588 maternal lines (S msms) and the fathers of maintainer (N msms). To obtain F1 hybrids with the maximum level of heterosis, the combined ability of lines and pollinators was evaluated by topcross and diallel crosses. The best lines for the complex of economically useful features with high combinational ability were included in various combinations of crosses with inbred lines in order to select high-performance hybrid combinations and to create F1 hybrids. 547 combinations of crosses of sterile lines with inbred paternal lines were carried out, on the basis of which 408 F1 hybrids were created. After testing and evaluation of them in the hybrid and breeding nurseries allocated 23. According to the results of the competitive test samples were identified, which under the names of Logran F1, Zarnitsa F1 and Solnyshko F1 transferred to the state variety test.
Afficher plus [+] Moins [-]SELECTION OF LEEK FOR THE MIDLAND OF RUSSIA AT CULTIVATION NO SEEDLING METHOD
2018
A. F. Agafonov | М. V. Dubova
Leek very ancient culture which was widely applied in cookery and as remedy still by ancient Egyptians, Greeks and Romans. Now the leek is widespread in countries of Western Europe and North Africa, in the USA, Canada, Australia. On chemical composition it is one of the most valuable onions representing in fact "the alive table of Mendeleyev". In such countries as Belgium, Holland and France it is one of the main vegetable cultures, and annual production it per capita makes from 4 to 10 kg. In Russia, unfortunately, it is still rare culture though falls into to the most valuable types of an onion on biochemical structure, productivity, resistance to wreckers and diseases. Widespread introduction of a leek in production restrains a number of factors among which lack of early ripening varieties with the increased resistance to extreme environmental conditions, and in this regard – difficulty of receiving high-quality seeds of leek in a midland of Russia to the north of Rostov-on-Don. Therefore, a vital necessity is on the basis of study under various conditions of cooperation a "genotype is an environment" and selection from world collection of sources of economic-valuable signs, creation of productive sorts and hybrids with good resistance to cold, possessing in the conditions of Moscow Suburbs, along with the high productivity and quality of products, by a high adaptivity to the different terms of height winter spending. Results of long-term researches of laboratory of selection of onions cultures (VNIISSOK) on studying, assessment and selection of exemplars of grades of leek from the VIR world collection are presented in article, receiving hybrid combinations and lines, development of methods of selection and creation on this basis of grades and hybrids of leek for not seedling culture in a midland of Russia, possessing high rates of vegetable and seed efficiency, quality of production and winter hardiness.
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