Relevance. Solanum tuberosum L. – it is one of the main food crops, therefore, the study of the influence of innovative growth regulators on physiological and biochemical parameters and plant yield is very important for the agro-industrial complex.Material and methods. The research was carried out on the gray forest soil of the Orel region in the conditions of a vegetation house on the basis of the farm Stebakov V.I. in the period 2020-2021. Potato of the Nevsky variety was used as the object of the study. Treatment with growth regulators was carried out by soaking Solanum tuberosum tubers in aqueous solutions of melafene (10-6%), Energy-M (10-3%) for 8 hours. When laying the experiments, the optimal amount of nitrogen (2.3 g), phosphorus (0.7 g) and potassium (3.1 g) was introduced into the soil for potato. Was determined catalase, peroxidase activity. The amount of hydroperoxides, an intermediate product of POL, was estimated by reaction with ammonium rhodanide, the content of malonic dialdehyde by reaction with thiobarbituric acid. The experiment was carried out three times. Statistical processing of the results was carried out by the method of one-factor analysis of variance using the computer program "MS Exel".Results. Studies have shown that there is an increase in the activity of catalase and peroxidase in all variants of the experiment compared with the control in leaves, in tubers – the activity of enzymes decreases. There was a decrease in the content of malondialdehyde in all variants of the experiment compared with the control. The analysis of the final productivity revealed that the total number of tubers on one bush of the potato in the variants with growth regulators changes slightly, while the mass of tubers increases, so the variant with the growth regulator melafen increased this indicator by 37% compared to the control.

This article shows the influence of growth regulators and methods of their application on the passage of the main interphase periods and growth processes during melon cultivation. The use of growth regulators is one of the promising directions in the cultivation of melons. In the acutely arid areas of the Volgograd Volga region, the use of mineral fertilizers for melon crops, in bagar conditions, was not effective, since there was an acute shortage of moisture in the soil. Therefore, the most effective and promising for creating favorable conditions for the growth and development of melon plants, with the possibility of leveling negative environmental factors, is the use of foliar treatments in the cultivation technology with growth regulators. The studied drugs of the new generation have a wide range of physiological activity. They are designed to influence the parameters of growth and development of plants as a whole in various phases, as well as their organs, as well as the parameters of plant response to environmental factors.Results. Studies have revealed the dependence of the growth processes of melon plants on the methods of using growth regulators, the length of the lashes in the variants of using growth regulators for processing plants during vegetation is longer compared to soaking seeds, with their maximum length in the Phytozont variant (plant treatment) is 12.7-51.3% longer compared to other studied preparations.

Relevance. Plants of potato varieties are carriers of viral pathogens in a latent form. These viruses can be transmitted to clonal progeny of the carriers. The system of virus-free seed production facilitates the elimination of the viruses in seed potatoes and preserves the high productivity of potato varieties. The research goal was to develop a scheme for virus elimination in potato using biotechnological methods and to introduce this scheme in the production of virus-free tubers under the conditions of Primorsky krai.Material and methods. New promising variety Moryak (breeding number Pri-08-11-1), which was created in FSBSI “FSC of Agricultural Biotechnology of the Far East named after A.K. Chaiki”, was used as the research object. The mean yield of the new genotype is 34.1 t/ha, the potential yield is 40.1 t/ha. The dry matter content is 18.13-23.85%, the starch content is 12.10-17.24%, and the content of vitamin C is 17.46-23.12 mg/100 g. This variety has a high keeping quality of tubers (92.2-94.4%) and resistance to excessive soil moisture. Tissue culture and chemotherapy in combination with ribavirin (a concentration of 0.02-0.03%) and chitosan (0.01-0.1%) were used for virus elimination. Sprouts from the original tubers and plantlets were tested by EIA and qPCR for latent infection (PVX, PVY, PVA, PVS, PVM, PLRV).Results. A sequential increase in the concentration of ribavirin (from 0.02 to 0.03%) and chitosan (from 0.01 to 0.1%) and their alternation in different passages proved to be an effective method for virus elimination in plantlets. As the result of the research, the new scheme for the elimination of the most economically important potato viruses was developed and introduced, and virus-free seed material was obtained.