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SYNERGISTIC EFFECT OF VOLATILE OILS AND ANTIBIOTICS AGAINST SOME GRAM POSITIVE AND NEGATIVE PATHOGENIC BACTERIA
2006
M.F. Ghaly
Eight most currently used antibiotics were examined for their antibacterial properties against Gram-ve bacteria as Pseudomonas aeuroginosa, E. coli, Proteus vulgari and Gram+ve as Staphylococcus aureus, Streptococcus pneumonia. Nitrofurantoin was the most effective against the tested bacteria, the inhibition zones ranged between 16-20mm and the MIC between 65-85ug/ml followed by ampicillin (11-18mm), ciprofloxacin (9-12mm) and gentamicin (6-9mm). The erythromycin was the lowest effective against the tested bacteria. Also, seven volatile oils were applied by contact and fumigation methods to study their effect on the tested bacterial strains. The fumigation method gave the highest inhibitory effect more than contact method and the thyme oil gave maximum inhibitory action (inhibition zone 20-28mm) against all the tested bacteria, and the MIC ranged between 0.1-0.15mg/ml followed by marjoram oil (19-25mm) and the MIC between 0.1-0.2mg/ml, cinnamon oil (12-16mm) and the MIC between 0.2-0.3mg/ml. Anise and chamomile oils did not gave any response against all the tested bacteria. The combination between thyme and other tested oils gave a synergistic effect for inhibitory action against all the tested bacteria, if compared with thyme oil alone. The combination between thyme and marjoram oil gave the maximum inhibition zones (20-29mm), followed by thyme with cinnamon oil (20-27mm), thyme with geranium gave (18-27mm), thyme with peppermint (17-27mm), thyme with chamomile (16-27mm) and thyme with anise oil (15-26mm). The combination of thyme oil with different tested antibiotics gave the lowest inhibitory effect than combination between thyme and other volatile oils against all the tested bacteria. The protein and DNA content of treated bacteria with thyme oil were increased by 38.46-47.37% and 34.26-46.94% respectivily, if compared by non-treated bacteria
Afficher plus [+] Moins [-]EFFECT OF BROAD BEAN MOTTLE VIRUS INFECTION ON FABA BEAN NODULATION AND EFFICIENCY OF N2-FIXATION
2006
Hanaa H.A.Gomaa | M.M Hazaa | Kh.A El-Dougdoug | M.H Abdel-Ghaffar
Plant growth and root nodules of infected Faba bean plants with broad bean mottle virus were significantly reduced in plant height, leaf area, dry matter, number and size of nodules in comparison with healthy ones. As well Leghaemoglobin content and Nitrogenase activity were reduced in infected nodules at 45 days after BBMV inoculation. The amount of N2-fixed as expressed by acetylene reduction was reduced considerably by 38.8%. Nitrogen content as an indicator for the efficiency of N2-fixation was reduced in shoots, roots and root nodules of infected nodulated plants as compared with healthy nodulated ones. Differences between bacteroids in healthy and in BBMV infected cells were no longer apparent. These differences include: (i) a decrease in endocytotic and exocytotic vesiculation on the membrane envelope of the bacteriods and on plasma membrane of bacteroidal cells, (ii) a decrease in number of vesicles in the space between the bacteriod and the membrane envelope. The possible significance of these changes relative to the decreased efficiency of N2-fixation was discussed.
Afficher plus [+] Moins [-]FARMERS NEED OF AN EXTENSION PROGRAM TO RATIONALIZE IRRIGATION WATER AND MANAGE NEW IRRIGATION SYSTEMS IN “DARB AL-ARBE’EN” PROJECT, AL-WADY AL-GADID GOVERNORATE
2006
M.M Moustafa Muawad
The study aimed at identifying both knowledge and skill levels of farmers in regard of technical recommendations of rationalizing irrigation water use and its various management systems, determine the deficit in their knowledge and skills degree in that regard as a step to propose an extension program that hope to remedy this gap. The study was conducted in Al-Wady Al-Gadid governorate on beneficiaries of “Darb Al-Arbe’en” project, with a random sample of 160 represent about 25% of total beneficiaries (640). Data were collected through a personal interview questionnaire during (August-September) 2005, and the following statistics were used: frequencies, percentages, arithmetic mean, standard deviation, simple correlation coefficients, F test and multiple regression analysis (Stepwise).
Afficher plus [+] Moins [-]CROSS RESISTANCE OF DIPEL 2x RESISTANT STRAIN OF PINK BOLLWORM PECTINOPHORA GOSSYPIELLA (SAUND.) TO SOME CONVENTIONAL AND BIOINSECTICIDES
2006
El-Zemaity M.S. | A.E. Bayoumi | Mona F. Rofail | Hemat Z. Moustafa
A field strain of pink bollworm Pectinophora gossypiella (Saund.) collected from Sharquia Governorate, Egypt was exposed to the selection pressure of the for-mulation of Bacillus thuringiensis subsp. Kurstaki, Dipel 2x under laboratory condi-tions. Resistance ratio attained 16-fold based on the susceptible strain after 14 gen-erations of selection. Study the response of Dipel 2x resistant strain to some insecti-cides indicated that there is no cross resistance to the conventional insecticides, es-fenvalerate, chlorpyriphos and thiodicarb or the bioinsecticides Ecotech and Agerin. These data may be emphasizing the possibility of rotation the Dipel 2x with these insecticides in pest control program of pink bollworm to manage resistance to Bt products.
Afficher plus [+] Moins [-]HEALTHY MODIFIED ZABADY
2006
El-Demerdash M.E. | Eman, L. Moustafa | M.E Hashem
Buffaloe's milk was used for the manufacture of Zabady. Control, Zabady made by using 3% of the regular starter. 1.5% of the regular Zabady starter was added to the other three parts, then 1.5% of Bifidobacterium bifidium, ABT or autolyzed S. thermophilus were added to the other three parts respectively. The result showed an increase in acidity of control zabady, while bifidobacterium decreased the acidity and curd tension, and increased pH value, coagulation time and synersis. Organolep-tic properties showed an improve in the flavour of zabady by using bifidobacterium in the end of storage compared to the other treatments.
Afficher plus [+] Moins [-]GROWTH, YIELD AND YIELD COMPONENT OF INOCULATED CHICKPEA AND FABA BEAN PLANTS AS AFFECTED BY USING METHYLOTROPHIC BACTERIA
2006
Sawsan Shehata | Wedad E.E. Eweda | H.H Abo Taleb | Heba O.M. Orf
Two pot experiments were performed in Giza research station, AgriculturalResearch Center (ARC) using 2 isolates of Pink-Pigmented FacultativelyMethylotrophic bacteria (PPFMs) originated from chickpea and Faba bean. Foliarapplication with PPFM isolates were conjugated with specific rhizobial inoculumand N-fertilization (15 Kg N Fed-1). Nodulation status, nitrogen fixation and growthyield and yield component were recorded. Results clearly indicated that Chickpeawas superior in its response to foliar application with PPFM.C. As it gave higherrecords of number and dry weight of nodules, dry matter and N-content of plants ascompared to Faba bean. A field experiment was also conducted in sandy loam soil atSouth EL-Tahreer province to investigate the effect of foliar application withPPFM.C strain + specific Rhizobia and N-fertilization on nodulation, growth andyield of chickpea legume plants. Results indicated that foliar application withPPFM.C in the presence of specific rhizobial inoculation scored significant increasesin economic turnover of chickpea in the range of 21-32% as compared to Nfertilizationat rate 50 Kg N Fed-1. Foliar application with 5 L Fed-1 in the presenceof 15 Kg N Fed-1 and specific rhizobial inoculation led to an increase of 518 kg fed -1productivity of seed yield , with economic turnover of 2491 L.E.
Afficher plus [+] Moins [-]SURVEY OF MITES ASSOCIATED WITH GRAIN RESIDUES AND MIXED FLOUR IN WAREHOUSES AND MILLS IN GREAT CAIRO
2006
A.E.A Mahgoob | A.I. Badawy | I.M Badoor
Twenty one mite species belonging to 3 suborders and 11 families were found in grain residues and mixed flour samples collected from warehouses and mills at Great Cairo. They were 12 pest species, 7 predatory and 2 parasitic species. Species commonly found in all inspected materials at all locations were Dermatophagoides farinae Hughes, Tyrophagous putrescentiae (Sch.) and Cheyletus malaccensis Oud. However Acrus siro (Lin), Acarophenax tribolii (New.) and Pyemotes herfsi (Oud.) were often abundant. The remaining species occurred sporadically in the collected materials. Actinedid and acaridid mites were “dominant” and “constant” in mixed flour and grain residues samples, the second group was the most abundant. Whereas gamasid mites was “recedent” and “accidental”. Mixed flour taken from Qaliobia Governorate harboured the highest number of mite species (15 species) followed by grain residues from Giza Governorate (13 species). While grain residues taken from Cairo Governorate harboured the lowest number (5 species).The highest infestation level with mites (2677.3 individuals) was recorded in mixed flour at Qaliobia Governorate, while the lowest one (1067 individuals) was found in grain residues at Cairo Governorate. Generally, mixed flour harboured more mite species than grain residues. Most of the collected mite species occurred during autumn and winter except P. herfsi and A. tribolii which were recorded during spring and summer, respectively. Dominance and frequency of occurrence, population fluctuations, effect of temperature and relative humidity on the population dynamics, also the relation between the predators and pests of mites were discussed in details.
Afficher plus [+] Moins [-]PROLINE ACCUMULATION IN DURUM WHEAT (Triticum durum Desf) UNDER WATER DEFICIT
2006
Chaib, Ghania | M. Benlaribi
Proline’s content was estimated in different organs of durum wheat; dry wheat seeds (caryopsis), seeds during germination, second and third leave’s level in different degrees of water alimentation: 75%, 50.0, 42.5, 35.0, 25.0 and 12.5% of the field capacity. Four genotypes of durum wheat Triticum durum DESF were studied from three different origins; Algeria, France and Mexico. Results showed that proline’s content was low in dry wheat and in seeds during germination, and increased by decrease of water supplies. This amino acid estimation during experimentation, allowed us to classify the studied genotypes into 3 main groups: - Genotypes with high proline accumulation (Mexicali). - Genotypes with low proline accumulation (Clairdoc). - Two intermediate genotypes MBB and OZ. These differences among genotypes can be exploited in parietal selection with relation towater deficit.
Afficher plus [+] Moins [-]EVALUATION OF SOME MALE TYPES AS POLLINATORS FOR BARHI DATE PALM cv. GROWN IN EGYPT
2006
A.H Al-Hamoudi | A.M. El-Hammady | I.M Desouky | A . Abdel-Hamid
Four different date palm male types were chosen to evaluate which male could be recommended to use as a pollinator for Barhi date palm cv. grown in Egypt. Three male types namely Ghanamy, Fard and Boyr were grown in the Fruit Trees Experimental Station at Dibba, Fojaira, UAE, and the fourth male was an individual Sewy satellite seedling grown in Egypt. Number of spathe/palm; spathe weight, length, & width ; pollen grains weight / spathe ; average of strand length as well as pollen grains germination % were determined for each male date palm tree from each male type . Moreover, pollen grains from each male type were used as a pollinator to evaluate their effect on fruit set and fruit quality of Barhi date palm cv. This study was carried out during both 2004&2005 seasons. Data showed that both Ghanamy and Fard male type were superior in their morphological characters as compared with Boyr or Sewy satellite seedling male type. Boyr male type showed the lowest values of morphological characters. On the other hand, there were no great differences in pollen grains germination percentage. Moreover, the results clearly indicated that Ghanamy pollen recorded the highest significant fruit set percentage and bunch weight (Kg) in both seasons of study. While, Fard pollen produced the highest fruit weight (g), length, diameter, flesh weight& thickness and flesh %. Boyr and Sewy pollen showed the highest ratio of fruit length / diameter in both seasons. Data also revealed that dates that were picked from bunches pollinated with Fard pollen had the highest total soluble salts (T.S.S) value and the lowest tannins content.
Afficher plus [+] Moins [-]SCREENING OF ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF SOME ACTINOMYCETES ISOLATED FROM CONSTRUCTED WETLAND SYSTEM
2006
A total of 174 actinomycete cultures were isolated from a constructed biological water treatment system (BIOWATSYST)established at Abu Attwa station in Ismailia city, Egypt and funded by the European Commission Grant No.IC18-CT97-0163. The isolates were identified to belong to eight genera; Nocardia, Streptomyces, Intrasporangium, Micromonospora, Nocardioides, Actinomadura, Nocardiopsis, and Thermomonospora. They were screened for their antibacterial, antifungal and cytotoxic activities against certain human and plant pathogens. Antimicrobial activities were determined by measuring bacterial and fungal growth inhibitions while cytotoxic activity was studied by using the Artemia salina bioassay. Thirty two percent of isolated cultures displayed antibacterial activity, 15% displayed antifungal activity and 9% displayed cytotoxic activity. Members of genus Streptomyces has recorded as the most frequent active isolates against tested bacteria (42%) and fungi (49%). However, the most cytotoxic activity was found with members of genus Nocardia (46%). Results evaluated the fact that actinomycetes isolated from such systems could be considered as promising source for antimicrobial and cytotoxic bioactive agents.
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