This study was conducted to demonstrate the effect of feeding on defatted soy bean and flaxseed flour as protein and phytoestrogen sources; broccoli and red cabbage as antioxidant sources; in addition to skimmed milk powder as a calcium source in prevention of bone loss and cell damage that usually accompanies aging process. Raw materials were subjected to proximate analysis and biologically evaluation on ninety six 4 month aged female albino rats. Eight rats were regarded as an initial group. Other eight rats were hold as a normal negative control group and fed on a basal diet. The remaining rats were ovariectomized and fed on low calcium diet for 8 weeks to produce osteoporosis model. Osteoporotic rats were divided into 9 groups (each of 8 rats) fed on standard diet supplemented with: I) soy bean flour, II) soy bean flour and broccoli, III) soy bean flour and red cabbage, IV) soy bean flour and mixture of broccoli and red cabbage, V) defatted flaxseed, VI) defatted flaxseed and broccoli, VII) defatted flaxseed and red cabbage, VIII) defeated flaxseed and mixture of broccoli and red cabbage. Skimmed milk powder and vitamin D were added to the diet of each tested group. One group was kept as a positive control and fed on a standard diet. The results showed a decreases in body weight, changes in relative weight of kidney and liver organs, physical properties of bone, also decreases in serum and bone minerals including; calcium, magnesium and phosphorus in osteoporotic model .A decreasing in estradiol and increasing in parathyroid hormone as a bone marker were also found. Results observed in tested groups showed that the highest recovery for the parameters were found in group IV which fed on standard diet supplemented with soy bean flour and mixture of broccoli and red cabbage followed by group VIII which fed on standard diet supplemented with defatted flaxseed and a mixture of broccoli and red cabbage. Therefore, it could be concluded that the aforementioned mixtures may be a promising sources to produce a functional food for prevention of bone loss and cell damage.

The effect of chitosan and salicylic acid treatments to investigate the seedling growth parameters of Broccoli seed sprouts (Tiburon f1) cultivar was the aim of this study. Salicylic Acid and Chitosan are the )elicitors( that stimulate the sprout, which are divided into Biotic (biological origin), abiotic (chemical or physical origin) elicitors and phytohormones have been applied alone or in combinations, in hydroponic solutions or sprays, and in different selected time points of the sprout growth or during post-harvest. Cumulative time had been completed (harvest time) using tap water, chitosan at 25, 50,100 ppm and salicylic acid at 65,130,260 ppm for seed soaking and sprouting , then precooled and air-dried sprouts were randomly chosen from glass jar (replicate) to collect data of sprout length, hypocotyl length, radical length, dry weight and fresh weight. Also, Samples of harvested 3, 5 and 7 days old etiolated broccoli dried using air draft oven at 65.5C° then grounded into powder for chemical analysis while fresh sprouts and seeds were analyzed for total phenols. However treatment with chitosan at 100 ppm cleared that the moisture, protein, ash, fiber, calcium , potassium, and total phenols of sprouts were increased by 9.63%, 33.73%, 11.80, 11.67%, 336.50 ppm, 196.43 ppm and 66.51mg/100g, respectively. Treated sprouts with salicylic acid at 65 ppm concentrate were also induced an increments on the same constituents reached 9.53%, 33.40%, 11.63, 10.46%, 333.50 ppm, 190.60 ppm and 62.90mg/100g, respectively over control (untreated). Therefore, treated sprouts with chitosan or salicylic acid could be considered as useful tool for improving the growth characters and bioactive metabolites of Broccoli seed sprouts For their Production of anti-cancer materials, As well secondary metabolic pathways respond to specific treatments with elicitors would be the basis for to enhance the production of secondary metabolites, in order to produce quality and healthy fresh foods