The objective of the present study was to eval-uate the effect of using one method of estrus syn-chronization programs on Jersy cattle reproductive performance under arid land environment, by ap-plying a specific doses of prostaglandin F2α (two injections of 5ml for cow), to increase the repro-ductive efficiency of the animals, through regulat-ing time of pregnancy and parturition. Forty non-pregnant, healthy cows were taken randomly from the original stock of Hada Al Sham Research sta-tion which belong to king Abdul Aziz University and were classified into two groups, treatment and control groups. Results obtained showed that: es-trus synchronization program using two injections of prostaglandin F2α showed that, statistically, there is no significant difference in the plasma progesterone concentration between treated and control groups. Jersy cows treated with prosta-glandin F2α showed estrus in a shorter period compared to the control group. The percentage of animals showed estrus was 75 ٪ in treated group, compared with 65% in control group which showed 84 ٪. The pregnancy rate in treated group was 86.66 ٪, versus 69.23٪ in the control group. Service period length (SPL) was 97 days for the treatedgroup, compared to 104 days for the control group. There is a significant (P0.01) difference in the plasma progesterone concentration between both groups during pregnancy, which was higher in the treated group.- The differences in the plasma progesterone con-centration between both groups after parturition were not significant. Estrus synchronization in the Kingdom of Saudi Arabia under arid land environment was considered as an application of new technology to improve reproductive effi-ciency of animals, and to regulate time of breed-ing and parturition in the herd. This will lead to a great important in the management of the ani-mal production branches.