Natural sources of water are limited in Egypt. The rapidly growing populations necessitate continuous expansion of the cultivated area. This means an increase of the gap between the demands of water for irrigation and the limited water supply. Looking for other sources such as low quality water like (industrial effluent, drainage and sewage) must be used in irrigation of some garden and wood plants. This investigation was conducted on agricultural drainage wastewater from El Mohete drain (Marioteya Canal) west of Cairo; the samples were collected from different places during (Summer and Winter seasons). The wastewater contaminated with pathogenic microorganisms, the excess of fertilizers (inorganic & organic), heavy metals, and the residuals of pesticides. Plant Growth Promoting Rhizobacteria (PGPR) could remediate the wastewater as biological bioremediation to remove some pollutants such as pathogenic microorganisms, heavy metals and pesticides. Chemical remediation was used as nitrification inhibitor to stop transformation of ammonia to nitrate. This work was conducted to study the ability of PGPR strains e.g. Bacillus megaterium, Bacillus subtillis, Bacillus circulans, Paenibacillus polymyxa, Pseudomonas floresense, Serratia sp. and Azotobacter chroococcum 5, 9 and 23 to treat the drainage water for irrigation the Mentha viridis cv. and Gladiolas grandiflorus cv. plants.                 Two pot experiments were conducted in greenhouse. The treatments were applied as follows; Natural water, treated water and drainage water to irrigate the plant. Use the PGPR as inoculants and thiourea as nitrification inhibitor, Heavy metal treated was (Copper, Cobalt, Zinc, Cadmium and Mercury) the result showed us heavy metals removal by PGPR from drainage water. The characterizations of PGPRs as shown in the obtained results are they could enhance plant growth by using their own metabolism (solublizing phosphate, producing hormones or fixing nitrogen) as well as correlation of them with the potenit of effects on the growth of plants in unfavorable conditions in order to improve the efficiency of phytoremdiation of contarinated soils. The removal of heavy metals and the elimination of pesticides residues were markedly noticed in this investigation. Results also confirmed the ability of PGPRs in suppressing the effect of pathogenic bacteria like Salmonellasp and E.coli. These abilities are of great importance in terms of plant and soil health. Consequently, the role of PGPRs bacteria associated with plant rhizosphere in remediation of water and soil contaminations due to its biochemical activity and thus, stimulate plant growth is a great important subject in phytoremediation process nowadays.

Concentration of heavy metals (Pb-Cd-Cr) was determined, that’s most polluted environment and rivers in fourteen varieties of vegetables throughout two growing seasons (2013-2014  &2014-2015), belonging to the three groups of vegetables (leaves and tuber and fruits), and using atomic absorption device, where the samples were collected from six different locations in Hama, and close to the course of the Orontes River, which vary different in irrigation operations and source of irrigation water.   Results of this study showed that no significant difference (P> 0.05) between the types of vegetable groups in the content of heavy elements at each locations seposatly. But it clearly showed the presence of very high significant differences (P <0.0001) in the contents of vegetables from heavy elements collected from sites, this effect is due to irrigation water. Also, the average concentration of cadmium was higher than the global natural concentrations in accordance with similar studies, the average lead, and chromium were lower than the averages of similar vegetables from other countries.

Dill ( Anethum graveolens L.) plants were cultivated during two successive seasons (2002/2003 and 2003/2004). The experimental were conducting at the Experimental Farm, National Research Center (NRC), Egypt, to study the effect of different combination of organic fertilizers such as chicken and sheep manure and its rates on dill ( Anethum graveolens L.) plants. The obtained results indicated that, treated plants with different combination of organic fertilizers and its rates resulted in a significant increase in growth and yield characters , i.e. plant height, branch number, leaf number, umbel number, fresh weight, dry weight and fruit yield / plant in comparison with control plants. Chemical composition analysis of treated plants showed an increase in the essential oil percentage and main components (Carvone (R) and Limonene) of essential oil extracted from dill (Anethum graveolens L.) fruits. Treated plants showed a decreases in the heavy metals content in the plant tissues comparison to untreated plants. Also these results indicated that the application of organic manure reduced the harmful effects of hazardous heavy metals

The present study measured the concentrations of heavy metals (Zn, Ni, Pb, Cu, Cr, and Cd) in soils as well as vegetables (faba bean, cabbage, onion, garlic, zucchini, capsicum, eggplant, potato, okra and green bean) irrigated with wastewaterinthe western part of Giza governorate, Egypt.Human health risks to consumers of these vegetables were performed. The study revealed the considerable variations inmetal contents of water, soiland vegetables samples. Generally, wastewater of the study drains was not suitablefor irrigation purpose according to the bacteriological guidelines. Almost all the physicochemical parametersand heavy metals concentrationsof water and soil samples were compatible with the recommended permissible limits of irrigation and agricultural use, respectively. Vegetable species showed remarkable difference in metals concentrations of various plant portion, Zn showed highest tissue concentrations followed byCu. Heavy metalsconcentrationsindifferent edible portionsdecreased in a descending order as Zn > Cu > Pb > Ni > Cr > Cd with low translocation values (< 1) for studiedvegetablesexcept forfaba bean.The health risk index (RI) for humans was low if edible portionsfrom studied vegetables are consumed, in particular Cd, Cr, Pb, and Ni whose concentration valueswere relatively higher than the health based guidelines values. Considering the probable health risk associated with the consumption of contaminatedvegetables, it is important toregularly monitor the levels of metals in wastewater, soils and vegetables in the studied area.

Azolla pinnata is a small aquatic fern which considered as a multipurpose organism. It isused in bioremediation to remove heavy metals from polluted draining waters. In view of its potentiality, Azolla pinnata were tested for their growth on different media (Yoshida, peat moss, and Soil media) to select the best medium for the growth of Azolla. Then the best medium was examined for the potentiality of Azolla to withstand a different concentration of different heavy metals. Yoshida medium was the most suitable medium, as it recorded the highest significant records in fresh and dry weight, being 25.38 and 1.69 g/ pot respectively, the doubling time was 5.43 days, NPK% being 3.87, 0.85 and 1.95% respectively and nitrogenase activity being 14.32 μmol C2H4/ g dry weight. / hr). The fresh and dry weight of Azolla exposed to Pb+2,Co+2 or Cu+2, was found to be increased by increasing the concentration of the metals from 5 to 35 ppm/liter, then they decreased when the fern exposed to 45 ppm/liter for all of them. While, doubling time decreased gradually by increasing the concentration of Pb+2 , Co+2  orCu+2 from 5 to 35 ppm/liter and started to increase by increasing the concentration of Pb+2 to 45 ppm/liter in the medium.

The sorption isotherm and kinetic of Cu2+, Pb2+, Zn2+ , and Cd2+ ions onto sewage irrigated soil (El-Gebal El Aasfar-Qalubia) was examined as a function of the retention time and initial ion concentration using batch equilibrium sorption experiments. Results show that the Cu, Pb, Zn and Cd metal ions onto the soil is relatively rapid and sorption reaches equilibrium at about 240 minutes. Kinetics of the sorption process on the soil is well characterized by the pseudo-second order reaction rate. Langmuir, Freundlich and D–R isotherm models are fitted for the sorption of Cu2+, Pb2, Zn2+, and Cd2+ ions onto the soil. The constants of all models were calculated for each metal ion and compared. It indicated clear differences between the sorption characteristics of Cu2+, Pb2+, Zn2+, and Cd2+ ions on the sewage irrigated soil.