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APPLICATION OF SOME STRAINS OF FLUORESCENT PSEUDOMONADS IN MANAGING ROOT-INFECTING PATHOGENS OF MAIZE
2018
O. Abdel Azeez | Enas Hassan | E. El-Assiuty | E. Ramadan
Possibility of manipulating some of the efficient strains of fluorescent pseudomonads to manage the root-infecting pathogens of maize was studied throughout this study. Out of 110 isolates 24 of Pseudomonas species, recovered from the plant rhizosphere showed to have inhibitory effect against two major root-infecting pathogens of maize, namely Cephalosporium maydis and Fusarium verticillioides in vitro. Pot experiment revealed that just 4 isolates could reduce infection with both pathogens and enhance the plant growth as well. Based on the genotypic identifications of these four isolates showed that they were: Pseudomonas putida strain Pau9, P. putida strainPau11, P. putida strain Psf3 and P. aeruginosa strain Psf9.
Afficher plus [+] Moins [-]RESPONSE OF YELLOW MAIZE PRODUCTIVITY AND QUALITY TO INTEGRATION BETWEEN MINERAL AND ORGANIC NITROGEN FERTILIZERS UNDER NEWLY RECLAIMED SOILS CONDITIONS
2018
Y. ElAbdkrem | R. Abdrabou | M. El-temsah | M. Abd El-hady
Two field experiments were carried out at a private farm (newly reclaimed soil) at Somusta city, Bani-Suif governorate, Egypt, during 2014 and 2015 growing seasons. These experiments aimed to evaluate the application of different nitrogen sources and their combinations for increasing the productivity and quality of yellow maize (zea mays). Results showed that: Maize plants fertilized with120 kg MN/fad. recorded the greatest leaves number/plant (12.67) and number of green leaves /plant (12.67) after 45 days from sowing, however, the applied application of 60 kg MN/fad + 60 kg ONP /fad gave the maximum number of leaves /plant (15.17 and 17.83) and number of green leaves /plant (15.17 and 16.33) at stages of vegetative growth after 60 and 75 days from sowing, respectively. application of 60 kg MN/fad + 60 kg ONP /fad gave the highest values of leaf area index (LAI) at all stages of vegetative growth 3.16, 3.98 and 6.39 from 45, 60 and 75 days from sowing, respectively. At harvest maize plants fertilized with 60 kg MN/ fad + 60 kg ONP /fad recorded the highest value of grains number/row (32.83) followed by 60 kg MN/fad + 60 kg ONC/fad. the highest values of ears number/plant were recorded with the treatments 60 kg MN/ fad + 60 kg ONC /fad, 60 kg MN/fad + 60 kg ONP/fad and 60 kg MN/fad + 60 kg ONF/ fad with the same value (2.00). Data also revealed that the highest value of grain weight/plant (274.49) was recorded at 60 kg MN/fad + 60 kg ONP/fad treatment. Application of 60 kg MN/fad + 60 kg ONP/fad produced significant maximum grain yield (3.05 ton/fad.), biological yield (6.37 ton/fad) and grain crude protein yield (296.83 kg/fad). Maize plants treated with 60 kg MN/fad + 60 kg ONP/fad gave the highest values of grain and straw nitrogen percentage by 1.17 and 0.42% respectively. In addition the highest values of grain and straw phosphor percentage (0.49 and 0.20 %) and potassium percentage (4.30 and 1.90%) respectively were recorded with 120 kg ONP/fad.
Afficher plus [+] Moins [-]IDENTIFICATION AND CHARACTERIZATION OF HEAT SHOCK PROTEINS IN FOUR EGYPTIAN MAIZE INBRED LINES (Zea mays L.)
2018
Fatma Mahmoud | M. Rashed | K. Khalil | M. Abou-Deif
The heat shock protein (HSP) family is one of the proteins universally accumulated under heat stress condition. Four Egyptian maize inbred lines (Zea mays L.) were studied to identify heat shock proteins under heat stress at 45°C as protein markers for detecting the thermo-tolerance inbreds. The protein fingerprints of four inbred lines were performed by grain total-soluble protein electcophoretic analysis. The protein electrophoretic analysis showed 18 bands in a distinct pattern of K1 and K7 inbred lines, while 17 bands were present in G342 and Rg59 inbred lines as another distinct pattern indicating different genotypes. The high temperature effect on four maize inbred lines exposed to 45°C for 2 and 4 hours at 14-days old seedlings besides control (25°C) was studied. Several protein bands varied between low and intermediate molecular weights were induced after exposing to heat stress at 45°C. Four bands of heat shock proteins with molecular weights of 82, 22, 17 and 10 kDa appeared in the inbred line K1 after exposing to 45°C for 2 and 4 hours which may be indication of thermo-tolerance. Four and seven bands were enhanced after exposing to high temperature for 4 hours at 45°C which appeared more concentrated in the patterns of K7 and G342 inbred lines, respectively.
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