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Stenotrophomonas rhizophila a Novel Plant-Associated Bacterium With Distinguished PGPRs Properties
2023
Elhosieny, Asmaa | Zayed, Mona | Selim, Shawky | Abdel Aziz, Norhan
Stenotrophomonas sp. is a plant-associated rhizospheric bacteria. It has the capability to enhance plant productivity. It acts as a mineral dissolution and growth promoter for plants under salt-stress conditions. The selected isolates revealed the general morphological and biochemical characteristics of Stenotrophomonas sp. All the isolates exhibited the highest growth density after 15 hr. at 30˚C, resistance to different levels of salinity, different pH levels, and different temperature degrees. Also, the isolates revealed their capabilities to solubilize phosphorus and potassium and to pro-duce different phytohormones. The isolate X.M9 exhibited the highest growth performance in the form of 1.376,1.326,1.292,1.053 and 1.059 OD in most salts’ concentrations compared to all tested isolates respectively. As well it (X.M9) exhibited the highest resistance to low temperatures 10 and 15oC being 0.917 and 1.354 OD respectively. The isolate X.M9 recorded the highest IAA of 83.73 mg/100ml compared to all the tested isolates. The isolate X.M9 were identified using 16 SRNA sequencing and submitted to the GenBank database under accession number No: OP050187 as Stenotrophomonas rhizophila.
Afficher plus [+] Moins [-]Productivity Improvement of Canola Genotypes Under Salinity Stress Conditions by Integration between Mineral and Nano-Scale forms of Nitrogen Fertilizer
2022
Mohamed, Doaa | Fergany, Mohamed | Elhabbasha, Elsayed | El-temsah, Mohamed
Two field experiments were conducted during in 2019/20 and 2020/21 seasons to study the impact of integration among mineral and nanoparticle nitrogen (N) fertilizer levels on yield traits and chemical characters of some canola genotypes grown under salt stress conditions. Four treatments of N fertilization (190 kg N/ha as recommended dose; 50% of the recommended, 95 kg N/ha+nano nitrogen (5 L/ha); 25 % of the recommended, 47.5 kg N/ha+nano-nitrogen (5 L/ha), and nano-nitrogen (rate of 5 L/ha). canola genotypes (Trabber, Agamax, and Serw4) performance were assessed under three levels of saline irrigation water (control, 2000, and 4000 mg L-1). Results showed that increasing salinity levels up to 4000 mg L-1 led to decreasing in all studied yield parameters compared with those of control (tap water). Trabber genotype excelled significantly in most of the yield characteristics. Integration between nanoscale and mineral nitrogen fertilizer, i.e. 95 kg N/ha+5 nano N L/ha) showed superiority over all applied N treatments, recording the highest values. It could be concluded that since application of 95 kg N/ha+5 nano N L/ha exploiting the nano form of N saves about 50% of applied nitrogen in canola under saline conditions. Accordingly, nanoparticles help the environmental pollution to be reduced.
Afficher plus [+] Moins [-]Productivity Improvement of Canola Genotypes Under Salinity Stress Conditions by Integration between Mineral and Nano-Scale forms of Nitrogen Fertilizer
2022
Doaa Mohamed | Mohamed Fergany | Elsayed Elhabbasha | Mohamed El-temsah
Two field experiments were conducted during in 2019/20 and 2020/21 seasons to study the impact of integration among mineral and nanoparticle nitrogen (N) fertilizer levels on yield traits and chemical characters of some canola genotypes grown under salt stress conditions. Four treatments of N fertilization (190 kg N/ha as recommended dose; 50% of the recommended, 95 kg N/ha+nano nitrogen (5 L/ha); 25 % of the recommended, 47.5 kg N/ha+nano-nitrogen (5 L/ha), and nano-nitrogen (rate of 5 L/ha). canola genotypes (Trabber, Agamax, and Serw4) performance were assessed under three levels of saline irrigation water (control, 2000, and 4000 mg L-1). Results showed that increasing salinity levels up to 4000 mg L-1 led to decreasing in all studied yield parameters compared with those of control (tap water). Trabber genotype excelled significantly in most of the yield characteristics. Integration between nanoscale and mineral nitrogen fertilizer, i.e. 95 kg N/ha+5 nano N L/ha) showed superiority over all applied N treatments, recording the highest values. It could be concluded that since application of 95 kg N/ha+5 nano N L/ha exploiting the nano form of N saves about 50% of applied nitrogen in canola under saline conditions. Accordingly, nanoparticles help the environmental pollution to be reduced.
Afficher plus [+] Moins [-]EFFECT OF IRRIGATION WITH SALINIZED WATER ON GROWTH AND CHEMICAL CONSTITUENTS OF “KALA-MATA” OLIVE CULTIVAR GRAFTED ONTO DIFFERENT OLIVE ROOTSTOCKS
2005
Sanaa, Laz
The most important black table olive (Olea europaea L.) “Kalamata” cultivar, grafted onto three different vegetative olive rootstocks[Picual (Pic), Frantoio (Fra) and Koroneiki (Kor) cvs.] was evaluated with 2-year-old grafted plants grown in sand clay soil, and received concentrations of salt mixture (NaCl, Na2So4, CaCl2, MgSo4 and KCl) making 6000 and 8000 ppm with SAR 12 of salinity level, for two seasons. Morphological and chemical parameters were investigated to determine the relative salinity tolerance of these rootstocks and to define possible reasons for any observed differences in the salt tolerance. However, results indicated that differences in response to salinity among rootstocks were observed primarily in morphological traits. Increasing of salinity level in irrigation water decreased, all of scion height and its rate of increase, leaf area, number of leaves/plant and fresh & dry weight of leaves and roots. But different rootstocks can affect the degree to which these pa-rameters is reduced under salinity, where Kalamata growth on Picual and Frantoio was considerably better than on Koroneiki at 6000 and 8000 ppm treatments. In-versely in untreated grafted plants Kalamata on Kornaki exhibited the best growth vigor, comparing with Kal/Pic or Kal/Fra plants, suggesting that a decrease of scion growth in untreated grafted plants is a salt tolerance quality transmitted by tolerant rootstocks. Salinity significantly decreased leaf chlorophyll (a) and (b) content of all grafted plants, but different content among rootstocks were noted. On the contrary, proline content increased in leaves of all treated plants, however, insignificant dif-ference was noted between rootstocks, yet the interaction between the two factors show that Kalamata on Picual and on Frantoio rootstocks recorded higher values of leaf proline content than Kalamata on Koroneiki rootstock. Leaves and roots Na+ and Cl- content of treated grafted plants showed an increment but to a different de-gree, comparing with the untreated (control) plants. There were differences among the grafted plants where Kalamata grafted on Koroneiki (the least tolerant cv.) most-ly affected by saline treatment and accumulated the highest content of Na+ and Cl- in leaves comparing with Kalamata on Picual (the most tolerant cv.) or on Frantoio (the Sanaa LazArab Univ. J. Agric. Sci., 13(2), 2005400moderate tolerant cv.). Tolerant rootstocks contained more Na+ in roots than in leaves. This response become apparent as salinity increased. Leaf N, P and K con-tents decreased in Kalamata leaves as influenced by different rootstocks and increas-ing salinity in irrigation water comparing with the control. Kal/Pic and Kal/Fra plants had higher N% in leaves than Kal/Kor plants, whereas Kal/Pic accumulated K more than the other plants, while P content in leaves of all grafted plants were insig-nificant in the two seasons. Based on the overall growth parameters and chemical composition in response to salinity, Kalamata grafted on Picual exhibited the great-est salt tolerance followed by those grafted on Frantoio whereas, Kalamata on Koro-neiki showed the poorest plants pertaining salt tolerance.
Afficher plus [+] Moins [-]IMPROVING THE FRUIT YIELD AND QUALITY OF CUCUMBER BY GRAFTING ONTO DIFFERENT ROOTSTOCKS UNDER SALINE CONDITIONS
2019
S. Mohammed | M. Ragab | H. Metwaly | S. Kabeel
Two experiments were carried out under low polyethylene tunnels which furnished with drip irrigation during the two successive winter seasons of 2014-2015 and 2015-2016 at Elbrollous area which represents the circumstance and conditions of coastal zone of north Nile Delta kafr Elsheikh Governorate. The objective of the investigation was to find out the effect of grafting by different cucurbita rootstocks on cucumber hybrid "ESHRAK" (Cucumis sativus, L.) on vegetative growth, flowering, yield and fruit characteristics, beside nutrient contents of cucumber plants. Results showed that grafted cucumber plants on Super Shintoza led to significant increase in vegetative growth,i.e., stem length, number of leaves, number of branches and leaf area,fruit fresh weight (g), number of fruits, total yield were greater with grafted cucumber plants on Super Shintoza followed by grafted cucumber plants on cv. Ferro. However grafting had no significant effect on potassium % of fruit on both seasons. The highest total yield was obtained by grafting cucumber plants on Super Shintoza rootstock followed by grafting on cv. Ferro rootstock by 130 % and 73 %, respectively in the first season and 160% and 147 %, in the second season respectively as compared with ungrafted cucumber plants. Accordingly, this study ensured that the use of rootstocks were more beneficial than ungrafted cucumber in both tested seasons.
Afficher plus [+] Moins [-]Stenotrophomonas rhizophila a Novel Plant-Associated Bacterium With Distinguished PGPRs Properties
2023
Asmaa Elhosieny | Mona Zayed | Shawky Selim | Ayman Yassen | Norhan Abdel Aziz
Stenotrophomonas sp. is a plant-associated rhizospheric bacteria. It can enhance plant productivity. It acts as a mineral dissolution and growth promoter for plants under salt-stress conditions. The selected isolates revealed the general morphological and biochemical characteristics of Stenotrophomonas sp. All the isolates exhibited the highest growth density after 15 hr. at 30oC, resistance to different levels of salinity, different pH levels, and different temperature degrees. Also, the isolates revealed their capabilities to solubilize phosphorus and potassium and to produce different phytohormones. The isolate X.M9 exhibited the highest growth performance in the form of 1.376,1.326,1.292,1.053 and 1.059 OD in most salts’ concentrations compared to all tested isolates respectively. In addition, it (X.M9) exhibited the highest resistance to low temperatures 10 and 15oC being 0.917 and 1.354 OD respectively. The isolate X.M9 recorded the highest IAA of 83.73 mg/100ml compared to all the tested isolates. The isolate X.M9 was identified using 16 SRNA sequencing and submitted to the GenBank database un-der accession number No: OP050187 as Stenotrophomonas rhizophila.
Afficher plus [+] Moins [-]Nitrogen Nutrition and Adaptation of Halophyte Chenopodium quinoa to Salt Stress
2021
Karema Tarek | Ibrahim Alshamy | Sayed Hussin | Sayed Eisa
There is little evidence that the nitrogen nu-trition supply at rates above or less than what is considered optimal in non-saline conditions improves growth and yield of halophyte crop cultivated under salt stress. Therefore, hypoth-esize of the present work was to find out the magnitude to which N could restore the harm-ful effects of salt stress on quinoa plants. A pot experiment was performed in greenhouse con-ditions to evaluate quinoa's response grown under water salinity treatments (0.0 & 200 mM NaCl) when nitrogen nutrition rates were limiting (50ppm), adequate (250 ppm), and ex-cess (450 ppm) to guide proper application rate of nitrogen fertilizer under salinity stress. The results indicated that, salinity caused a signifi-cant decrease in the vegetative growth of the plant. Consequently, all vegetative measure-ments were negatively affected. As a result, the seed yield decreased to more than 50%. The application of a moderate level of nitrogen (250 ppm) caused a significant ameliorative effect on seed yield by 126% under non saline conditions and 34.5 % under saline conditions compared to the low nitrogen level. The results did not improve any further with the applica-tion of a higher level of nitrogen. These results indicate that applying (N) in adequate may im-prove most traits and prove to be a physiolog-ical treatment to increase resistance against the negative effects of salt stress in quinoa.
Afficher plus [+] Moins [-]DEVELOPMENT OF SOME TOLERANT YEAST (SACCHAROMYCES CEREVISIAE) STRAINS TO HEAT AND SALT STRESSES
2019
Sara Abd El-Maksoud | M. Sayed | Fatma Badawy | Eman Fahmy
All living organisms are subjected to changing in conservational conditions, to which they must adapt to. Stress is defined as a threat refers to the physiological balance of systems critical to survival organisms. Five yeast strains (Saccharomyces cerevisiae) were subjected to different adverse environmental situations, such as thermal, osmotic and oxidative (salt) stresses. The objective of this work was to detect the most tolerant yeast strains under salt or heat stresses. Five yeast strains were exposed in a first experiment to heat stress at 20°C or 40°C beside to the control at 30°C to detect the more tolerant strain. The same yeast strains were subjected in a second experiment to two different concentrations of salt stress (NaCl); 0.5 or 1.0 M, separately, for two days (at 30°C as normal temperature for growth), other strains were exposed to 0.5 M concentration of NaCl for 24 hours, then 1 M for another 24 hours. For the heat stress results, strain S4 was more tolerant at 40oCwith insignificant difference compared to the control (30oC), while it showed significant difference at 20°C. Strain S5 also was more tolerant at 20oC with insignificant difference compared to the control. For salt treatment, the only insignificant value was for strain S3at 0.5M NaCl compared with the control.
Afficher plus [+] Moins [-]ENHANCEMENT OF SALT TOLERANCE IN WATERMELON USING GRAFTING
2018
Asmaa Sharf-Eldin | Amal El-Shraiy | M. Eisssa | Sanaa Zaghlool
Pots experiment was conducted in the experimental farm of Agricultural Botany Department, Faculty of Agriculture, Ain Shams Univ. during the seasons of 2015 and 2016 to investigate the effect of grafting on salt tolerance of watermelon plants. Watermelon (Citrullus lanatus, Hybrid F1) Aswan1 was grafted onto the rootstock of Flexifort pumpkin (Cucurbita maxima x C. moschata) and irrigated with four different concentrations of NaCl (0.0, 2000, 4000, 6000 ppm). Two samples were taken at 20 and 40 days after planting (DAP). Plant height, leaf numbers and area, branches number, root length, and shoot and root fresh and dry weights were negitavly affected by salinity in ungrafted plants and this effect was directly proportional to NaCl concentrations. On the contrary, grafting positively affected the aforementioned parameters and minimized the harmful effect of salinity. Furthermore, grafted plants showed higher growth vigor comparing with ungrafted control plants or plants received the same treatment of NaCl and these effects were mostly siginificant. An increase in membrane permeability (MP) was detected at 20 and 40 DAP by application of different levels of NaCl salinity and this effect was positively correlated with NaCl concentration. Grafted plants showed decreasing in MP with 12.7% higher LRWC than ungrafted plants. Under 2000, 4000, 6000 NaCl ppm salinity levels, the values of salt injury index recorded 15.1, 26.5 and 37.5 in ungrafted plants at 20 DAP comparing with 0.0, 6.9 and 12.9 in grafted ones.
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