Faba bean (Vicia faba L.) seeds cv. Misr 1  were treated with Bion (5 mM), salicylic acid (5 mM) as chemical inducers as well as Paenibacillus polymyxa and Trichoderma harzianum as biotic inducers to study their effect on the infection with Rhizoctonia solani and Fusarium solani f. sp. fabae under greenhouse and field conditions.           Under greenhouse conditions (Agricultural Research Centre, Giza) all treatments induced reduction in the percentages of pre- and post-emergence damping off caused by R. solani or F. solani f. sp. fabae compared to untreated control. The highest percentage of survived plants was achieved from the inducer Bion (92 % and 88%) compared with untreated control (40% and 36%) in the presence of R. solani or F. solani f. sp. fabae respectively. Meantime, there was no significant difference between the Bion and the fungicide Rizolex –T (3 g/Kg seeds) treatments. However, all treatments caused significant increase in the figures of plant height, shoot and root dry weight, nodules number/plant and nodules dry weight over the control treatment grown in infested soil by R. solani or by F. solani f. sp.  fabae. The highest values of all growth parameters under study were recorded on faba bean plants check (healthy plants) grown in disinfested soil followed by Bion and Rizolex –T treatments in the presence of R. solani or F. solani f. sp.  fabae. There was no significant difference between Bion and Rizolex-T treatments with exception of nodules number and nodules dry weight /plant in the presence of R. solani. On the contrary, there was significant difference between Bion and Rizolex-T treatments with exception of plant height in the presence of F. solani f. sp.  fabae.           Under field conditions at Giza (Giza Governorate) and Etay El-Baroud (El- Behira Governorate) Agricultural Research Stations during winter season 2013-2014, all the treatments significantly decreased the percentage of pre- and post-emergence damping-off and increased the percentage of survived plants compared with untreated control in two locations with exception of post-emergence percentage figures at Giza Agricultural Research Station. Regarding survived plants, there were no significant differences between the Bion (89.5% and 86.5%) as well as Rizolex-T (90.9% and 88.8%) treatments compared with untreated control (68.3% and 64.3%) at Giza and Etay El-Baroud Agricultural Research Stations respectively. However, all inducer treatments significantly improved growth parameters (i.e. plant height, number of pods/plant, seed weight /plant and one hundred seed weight) as well as yield compared to the untreated control in the two locations. Higher increase in seed yield (ton /feddan) was obtained with Rizolex-T and Bion treatments (62.2% and 57.9%) respectively, followed by salicylic acid and P. polymyxa with (39.4% and 28.4 %) respectively, increasing over the untreated control calculated as means of the two locations.         Activities of peroxidase (PO), polyphenol oxidase (PPO) enzymes and phenol content were determined in faba bean plants treated with different inducers. Bion treatment showed the highest increase in PO and PPO activity, total and free phenols followed by salicylic acid treatment in the presence of R. solani or F. solani f. sp.  fabae.

Preparationof either Bacillus thuringiensis or Trichoderma harzianum  were used as seed coating or soil application for management of root-knot and root rot disease complex caused by the root-knot nematode, Meloidogyne javanica and the fungus Rhizoctonia solani on soybean plants. Number of galls, root galling, egg masses and disease severity were reduced sharply on plants treated with both biocontrol agents, either as seed or soil application compared with non-treated plants. Soil application with both biocontrol agents was the best method in reducing nematode and fungus disease severity. Plant growth parameters i.e. fresh shoot and root weight, dry weight and number of pods and bacterial nodules were markedly increased with all treatments of biocontrol agent either with seed or soil treatment compared to plants grown in infested soil with either pathogens alone or combined. Results show that plant survival was more affected when R. solani was alone or combined with M. javanica. Seed coating was considered the effective method in increasing survival plants. The biochemical analysis of treated plants with both biocontrol agents were affected compared to non-treated plants grown in pathogen infested soil. The biochemical parameters i.e. chlorophyll A and B, carotein, phenols and amino acids were enhanced in bioagent treated plants compared to non-treated plants