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TAXONOMIC STUDY OF TWO GENUS IN LICE Linognathus Africanus (Kellogg And Paine, 1911) & Bovicola (Damalinia) ovis (Schrank, 1781) BY SCANNING ELECTRONIC MICROSCOPE (SEM)
2011
Sara A. Aljubran | Souad M. Alsaqabi
This study presents the Mallophaga and Anoplura lice which affects farm animals (camels sheep and goats), a local area east of the Saudi Arabia of different ages, races, classes. Study the classification of lice were recorded as using the optical microscope and electronic scanning. The study shows scanning electron microscopy micro-structure of the species: Bovicola(Damalinia) ovis, Linognathus africanus and the study showed the installation of a portfolio of head lice, and the distribution of filament head, chest and abdomen, as shown over the centuries, the difference in the form of remote sensing and Swallows in the sexual lice, the first study in this area.
Afficher plus [+] Moins [-]MICROPROPAGATION OF DAMASK ROSE (Rosa damascena)
2011
Alsemaan T.; N. Albatal | Almaarri Kh.
This study aimed to establishing a protocol for producing in-vitro plants of Rosa Damascene. The culture was conducted at the Commission of Biotechnology in Damascus. Four types of explants were cultured on MS medium and some factors affecting culture were examined. The results showed that no viruses were observed, the lateral buds were superior over other explants, then the lateral microcuttings, after that, the apical micro-cuttings, and, finally, the shoot tips. The highest multiplication rate was observed at the hormonal combinations of (benzyl adenine BA 3mg/l with indole-3-acetic acid IAA 0.1 mg/l), and the highest elongation average were observed at ( IAA 0.1 mg/l with BA 2-6mg/l) or ( indole-3-butyric acid (IBA) 0.1 with (BA) 5-6 mg/l). The transferring was positively effective. The highest rooting percentage was observed when naphthalene acetic acid NAA or IBA were used. (Berlite: peatmoss, 1:1) was the best growing medium for hardening.
Afficher plus [+] Moins [-]EFFECT OF SOWING DATES OF THREE COWPEA CULTIVARS ON THEIR INFESTATION RATE WITH COWPEA POD BORER ETIELLA ZINCKENELLA
2011
A.A.R Helalia | Ali F.A.F. | Hegab M.F.A. | Kamal Kh.A.
Field trials were conducted during 2005 and 2006 seasons at Minofia governorate to evaluate the effect of planting date of three cowpea cultivars on their infestation rate with cowpea pod borer, Etiella zienckenella. For each cowpea cultivar planted at each tested date, the numbers of bores and larvae were counted in green and dry pods as well as in dry seeds and the means were obtained to estimate the degree of insect infestations. Results indicated that, regardless the planting date in both seasons, Kream7 was the highest resistant cultivar to insect infestation followed by Kaha1 and then Kafr El Shikh1. On the other hand, regardless the cowpea cultivar, the rate of insect infestation was greatly reduced at the early plantation. Thus, selection of Kream7 cultivar and early plantation could be involved in reducing E. zinckenella infestation and subsequently increase the cowpea yield. These studies clearly demonstrated that several non-insecticidal approaches have great potential for cowpea pod borer Etiella zinckenella management. These approaches include some of integrated pest management strategies which can effectively prevent or reduce infestation
Afficher plus [+] Moins [-]EFFECT OF NEEM AND WILLOW AQUEOUS EXTRACTS ON FUSARIUM WILT DISEASE IN TOMATO SEEDLINGS: 1-INDUCTION OF ANTIOXIDANT DEFENSIVE ENZYMES
2011
, R.M Farag Hanaa | Abdou Zeinab A. | Salama Dawlat A. | Ibrahim Mervat A.R. | Sror H.A.M.
Fusarium wilt disease is one of the major plant diseases that affect tomato production. The effects of neem (Azadirachta indica) and willow (Salix babylonica) aqueous extracts on fusarium wilt disease in tomato seedlings were investigated. Four weeks old tomato seedlings were treated with 10% of either neem and willow aqueous extracts and then infected with Fusarium oxysporum after 4 days of treatment. The results showed that the percentage of disease incidence was increased in non treated tomato seedlings in time dependent manner and reached the maximum level (65%) after 6 weeks of infection. Treatments of tomato plants with neem and willow aqueous extracts reduced the percentage of disease incidence to the level of 25.5% and 27.8% after 6 weeks of infection respectively. The results show that infection of tomato seedling with Fusarium oxysporum led to many morphological and biochemical changes including, reducing the growth of tomato shoot and root, increasing the level of lipid peroxidation and marked increase in the activities of antioxidant defensive enzyme i.e. POX, CAT, and SOD. Treatment with neem and willow aqueous extracts significantly exhibited a growth promotion of tomato shoot and root in infected or non infected seedling. Moreover, application of neem and willow aqueous extracts with fusarium, significantly reduce the level of lipid peroxidation and induce high activities of antioxidant defensive enzymes after 3 and 7 days of infection. Electrophoretic pattern of POX demonstrated that Fusarium oxysporum caused up regulation of several POX isoenzymes. It could be concluded that neem and willow aqueous extracts reduced the disease incidence of fusarium wilt in tomato seedlings by increasing the activities of antioxidant defensive enzymes and decreasing the level of lipid peroxidation.
Afficher plus [+] Moins [-]ZINC AND MANGANESE SORPTION BEHAVIOR BY NATURAL ZEOLITE AND BENTONITE
2011
A.L. Iskander | Khald E.M. | Sheta A.S.
Understanding the sorption process in natural zeolite and bentonite is necessary for effective utilization of these minerals as nutrient adsorbents and on the other side for controlling release of plant nutrients. This research was undertaken to characterize the ability of natural zeolite and bentonite minerals to adsorb and release zinc and manganese. The potentials for sorption of these ions were evaluated by applying the Langmuir and Freundlich equations. The results showed that Langmuir constant [maximum adsorption (b mmol kg-1)] of both Zn and Mn were for bentonite > zeolite While the [binding strength values (k in L.mmol-1)] for zeolite > bentonite Zn in case of zeolite showed the lowest desorbed percentage particularly at high levels of sorbed Zn. Only, 74.7 % is readily extractable by DTPA after three successive extractions leaving, 25.3 % Zn retained by the mineral. In case of bentonite, 82.26 % of sorbed Zn is readily extractable by DTPA after three successive extractions leaving, 17.74 % Zn retained by the mineral. In case of Mn, 84.63% of Mn sorbed by zeolite is readily extractable by DTPA after three successive extractions leaving, 15.37% Mn retained by the mineral. In case of bentonite, 89.79 % of adsorbed Mn is readily extractable by DTPA after three successive extractions leaving, 10.21 % Mn retained by the mineral. This finding may reflect clearly the possibility of using natural zeolite and bentonite as a slow release fertilizer for Zn and Mn and on the other hand preventing soil pollution with heavy metals.
Afficher plus [+] Moins [-]CELLULASE PRODUCTION BY TWO FUNGAL STRAINS ISOLATED FROM TAIF IN SAUDI ARABIA
2011
A. Sohair | A. Hussein Nemmat | A. Abozaid Abeer | Al-salemi Fawzia A.
Among 17 fungal isolates isolated from soil of El-hawia, El-hada, El-kaym and Karwa in Taif governorate in Saudi Arabia, two isolates showed high efficacy in producing cellulases enzymes. They were identified to be Alternaria alternata and Aspergillus wentii. Some factors such as carbon and wheat bran as a raw material, nitrogen, pH and incubation temperature were investigated. Results indicated that glucose and cellulose were the most effective as a carbon source while, urea was the best nitrogen source for cellulases production. Initial pH 5.0 and incubation temperatures at 25 or 35ºC achieved high cellulases production.
Afficher plus [+] Moins [-]ULTRASTRUCTURE OF TWO GENUS Przhevalskiana silenus (Brauer, 1858) AND Cochliomyia hominivorax (Coquerel, 1858) WHICH AFFECTS GOATS BY MYASIS IN SAUDI ARABIA
2011
Sara Aljubran | Souad Alsaqabi
Ultrastructure study revealed two types of fly larvae Przhevalskiana silenus (Brauer, 1858) and Cochliomyia hominivorax (Coquerel, 1858) that causes myasis in Goats in Saudi Arabia, The study recorded that the exact composition of these two genus showed differences in morphological characteristics, which cannot be identified using an optical microscope. All previous studies recorded on the same region never before carried out ultrastracture studied.
Afficher plus [+] Moins [-]Cellulase production by local bacteria isolated from taif in Saudi Arabia
2011
Nasr, S.A. | Abozaid, A.A. | Hussein, Y.A. | Al-Salemi, F.A
Among 20 bacterial isolates isolated from the soil of El-hawia, El-hada, El-kaym and Karwa in Taif governorate in Saudi Arabia,two isolates had high efficacy in producing cellulase enzyme. They belonged to genus Bacillus (Bacillus 8 and 17). Some factors such as carbon source and wheat bran as a raw material, nitrogen source, pH and. incubation temperature were investigated. Results indicated that CMC and cellulose were the most effective as they enhanced cellulases production .Sodium nitrate and ammonium chloride were the best nitrogen sources for cellulases production. Initial pH 7.0 was found to be optimal for growth and cellulase production. Incubation temperatures at 25 - 40°C achieved high cellulases production by the two isolates.
Afficher plus [+] Moins [-]Microbial production of thermoalkaliphilic enzymes from El-Khorma governorate for application in biodetergent technology
2011
Bayoumi, R.A. | Atta, H.M. | El-Sehrawey, M.H. | Selim, Sh.M.
Detergents are an undetectable source of pollution, which hidden in most of our daily activities. Detergents could cause harmful effects before they are completely degraded. It is wise to reduce the amount of detergents that usually used by invention new bio-friendly formula contains efficient enzymes such as protease. Screening studies were carried out for one hundred and fifty bacterial isolates with respect to their ability to produce protease(s), after growing on slaughter house wastes (SHW) isolated from EI-Khorma governorate,Taif, Kingdom of Saudi Arabia (KSA) at 55°C, and pH 9. The most potent thermophilic bacterial isolate concerning of alkaline thermostable protease(s) production was identified as Bacillus licheniformis EGT50. Alkaline thermostable proteases productivity by the most potent bacterial isolate was affected by substrate concentrations (solid substrate), carbon source, nitrogen source, amino acid supplements, incubation temperature, incubation period, and inoculum size. Maximum both enzymes production by B. licheniformis EGT50 was obtained on SHW concentrations, 7.5 %; galactose; diammonium hydrogen phosphate; arginine at 55°C for 72 h. when inoculated by 0.5 ml. The protease production under all optimal conditions was increased many folds from 563.68 to 17825 U/ml (31 fold). The purification fold of B. licheniformis EGT50 alkaline thermostable protease increased to 394.7 after applying Sephadex G200 column chromatography techniques. The enzyme productivity of protease has been determined and the result proved the possibility to use the crude and purified enzymes in biodetergent technology.
Afficher plus [+] Moins [-]Physiological response of strawberry grown in root-knot nematode infested soil under different safety control applications
2011
Mahdy, M.E. | Midan, S.A.
The evaluate some safety treatments including: mulching, tunnels, natural yeast extract, antioxidant and two rhizobacteria Serratia marcescens and Pseudomonas fluorescens towards root-knot nematode Meloidogyne spp. on strawberry plant grown in naturally infested soil and consequently their probable effects on growth parameters, chemical components and yield of strawberry compared to untreated control. Results of this study confirmed that all used treatments, significantly reduced all related nematode parameters i.e. number of galls/root system; number of egg masses/root system as well as number of juveniles/250 g soil (either after 2 or 4 months) when compared to plants grown in the naturally infested soil only. The highest percentage of reduction in all related nematode parameters were noticed after 4 months. Mulching, S. marcescens, P. fluorescens and tunnels, played an important role in reducing the percentage of nematode parameters. Mulching and S. marcescens reduced the percentage of gall numbers/root system by 77%, followed by tunnels by 75% and P. fluorescens by 70%. S. marcescens reduced the percentage of egg masses/root system by 80% followed by P. fluorescens by 78% and mulching by 76%. Results indicated that the soil treated with mulching, also significantly reduced the percent age of juvenile numbers/250 g soil by 79% followed by S. marcescens by 73%. . Results revealed that all previous treatments significantly increased the early and total yield. Our results confirmed that, the rhizobacterium S. marcescens came in the first rank as a promoter of all related plant growth characters, followed by tunnels and mulching treatments. Results confirmed that also the antioxidants, are considered the effective one in enhancing the activities of peroxidase and phenoloxidase as well as T:S.S% and V.C followed by the natural yeast extract.
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