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INFLUENCE OF SPROUTING USING BIOTIC AND ABIOTIC ELICITORS ON CHEMICAL COMPOSITION OF RADISH SEEDS (RAPHANUS SATIVUS) Texte intégral
2019
Islam Tork | A. Abdelhafez | Fatma Mostafa | M. Abdallah
Germination is a way to improve agricultural productivity and easily to use by low income families, in particular with using some elicitors in germination for enhancing the nutrition value of the seeds by sprouting. For their highly metabolic activities after harvesting, radish seeds were selected for performance of this study. The effect of using abiotic elicitor (saline water, by NaCl at different concentrations) and biotic elicitor (Saccharomyces cerevisiae) on sprouting of radish seed has been investigated. After germinating radish seeds for six days using elicitors, chemical analysis and determination for phytochemicals contents have been carried out. Results showed a promising efficiency by sprouting, where an appreciable increasing in some analysis as protein, carbohydrates, some minerals and amino acids comparing to seed. Besides, germination had a positive effect to present some phyto-compounds as some flavonoids, terpenoids and phenolic compounds. Then, this study and similar ones are an important step towards the future development of value-added foods with elicited phytochemicals and can be used in the development of innovative food products with beneficial effects on human’s health.
Afficher plus [+] Moins [-]LIPID METABOLISM IN DIABETIC RATS AS AFFECTED BY CANOLA AND MUSTARD SEED SPROUTS Texte intégral
2019
Hanaa Amer | Tahany Aly | K. Tobgy | M. Abdallah | N. El-Shahat
Canola (Brassica juncea L.) and mustard (Sinapis alba L.) seed sprout effects on diabetic rats have no available information and to clarify their effects, both sprouts were investigated in streptozotocin (STZ) induced diabetic and normal rats. Rats were fed on a semi-modified diet containing 10% of canola or mustard sprouted using tap or saline water for sprouting ad-libitum for 6 weeks. STZ showed increases in blood sugar, low density lipoprotein cholesterol (LDL-c), vary low density lipoprotein cholesterol (VLDL-c) and triglycerides (TG). The addition of canola and mustard with or without salinty at 10% to diabetic rats diet as semimodified diet resulted a significant decrease in blood glucose, TG and VLDL-c and data was more pronounced using mustard sprouted or saline water without changes in the HDL-c parameter. These results showed that canola and mustard especially mustard sprouted in saline water had a hypoglycemic activity in diabetic rats and partly improved lipid metabolism in the experimental rats, with non-toxic to rats in doses given over 6 weeks period in this study.
Afficher plus [+] Moins [-]ENDOPHYTIC BACTERIA ASSOCIATED WITH SUGARCANE AND ITS POTENTIAL TO SUPPRESS SPORISORIUM SCITAMINEUM, THE CAUSAL FUNGAL PATHOGEN OF SMUT DISEASE Texte intégral
2019
Shadia Taghian | Afaf El-Meneisy | A. Esh | N. Abdel-Ghaffar
Bacterial endophytic ubiquitously colonize the internal tissues of the plant. The purpose of this study was to isolate and identify the endophytic bacteria from sugarcane stalks which have antagonistic activity against Sporisorium scitamineum, the causal pathogen of sugarcane smut disease, and to study their activity to produce some secondary metabolites i.e. enzymes and growth-promoters. Sugarcane samples were collected from five governorates in Upper-Egypt namely, Giza, Beni-Suef, Sohag, Qena, and Luxor. A total of 240 isolates of endophytic bacteria were isolated from 160 samples of healthy sugarcane stalks (variety GT-54-9). Isolated endophytes were screened for its antagonistic activity against S. scitamineum, in vitro, using dual culture method. Only, 62 isolates showed different degrees of antagonistic activity. Ten isolates of endophytic bacteria were selected to study their potential to produce enzymes (chitinase, β 1,3 glucanase, phenylalanine ammonia lyase) and growth-promoters (siderophores, indole acetic acid (IAA), salicylic acid (SA)). These isolates were pre-identified using the morphological and physiological properties according to Bergy's manual as Pseudomonas sp., Serratia sp., Enterobacteria sp., Herbaspirillium sp., Gluconacetobacter sp. Anabaenopsis sp. and Azospirillumsp. All endophetic bacteria produced β 1,3gluconase and indole acetic acid (IAA), while Pseudomonas sp. produced salicylic acid and phenylalanine ammonia lyase, Ana baenopsis sp. produced phenylalanine ammonia lyase, Gluconacetobacter sp. produced chitinase and Herbaspirillium sp. produced siderophore. Meanwhile, isolates of Anabaenopsis sp. and Pseudomonas sp. were the most effective to produce β 1,3glucanase and indole acetic acid compared with other entophytic bacteria.
Afficher plus [+] Moins [-]EFFECT OF SOAKING AND SPROUTING USING SALINE WATER ON CHEMICAL COMPOSITION OF WHEAT GRAINS Texte intégral
2019
T. Hussein | Y. Abd El-Shafea | U. El-Behairy | M. Abdallah
In the current research, wheat grains were used to study the effect of grain soaking and sprouting using tap water and saline water (NaCl solution) on sprout growth, proximate analysis, minerals content, anti-nutritional and antioxidant compounds of sterilized grains (soaked for 0.33h) and soaked grains for imbibition (12h) and sprouted grain for 24h old. Results revealed that the longest radical of 24h old wheat sprout was observed at 2000 ppm NaCl, and shortest was observed at 4000 ppm NaCl. Soaked wheat grains (12h) for imbibition recorded the highest moisture content (10.2 to 10.9%) while soaked for 20 min (0.33h) in calcium hypochlorite for sterilization recorded medium content (8.8 to 9.9%) and the lowest one recorded in 24h old wheat sprouts (6.9 to 7.2%). The low moisture content the high total carbohydrate, total fats and energy and vice versa. Soaked grains for sterilization period (0.33 h) and imbibition (12h) increased zinc (Zn), manganese (Mn) and calcium (Ca) while non-sterilized only potassium (K). Tap water increased sprout magnesium (Mg), and manganese (Mn) content while saline water increased sprout magnesium (Mg), and calcium (Ca) content. Grain sprouting was effective in reducing phytic acid, oxalate and alkaloids anti-nutrient in wheat sprouts especially when using sterilized grains. Soaking non sterilized grains for imbibition (12h) in saline water contained higher total phenol, flavonoids and total antioxidant. Etiolated wheat sprouts contained lower total flavonoids and antioxidant compared with soaked grains in saline water.
Afficher plus [+] Moins [-]DIRECT EMBRYOGENESIS AND INDIRECT ORGANOGENESIS OF DATE PALM (PHOENIX DACTYLIFERA L.) CV. SEWI USING IMMATURE FEMALE INFLORESCENCES Texte intégral
2019
Mervat Malhat | H. El-Wakeel | A. Abd El-Hamid | S. Khalil | Mona Hassan
This study was achieved at the Tissue Culture Laboratory of the Agricultural Genetic Engineering Research Institute, Giza, Egypt during the period from 2013 to 2017, Direct embryo initiation and callus formation of date palm cv. Sewi from immature female inflorescences have been achieved on modified MS medium supplemented with 4 mg l-1 Picloram plus 3 mg l-1 2 iP and 2 g l-1 PVP. Results also showed that BA at 0.5 mg l-1 produce the highest number of differentiated embryos/culture, while kinetin at 0.25 mg l-1 significantly increased the average number of adventitious shoots/culture. NAA at 1.0 mg l-1 induced the highest rooting percentage and micro-shoot length. On the other hand the best survival percentage during the acclimatization stage was observed with plantlets produced from IBA at 0.5 mg l-1 during the rooting stage.
Afficher plus [+] Moins [-]IN VITRO PROPAGATION OF PLECTRANTHUS BARBATUS ANDREWS AS IMPORTANT MEDICINAL PLANT Texte intégral
2019
Dina Mahmoud | Lamyaa Sayed | M. Diab | Eman Fahmy
Plectranthus barbatus Andrew (Coleus forskolii) is one of the important species of the genus Plectranthus (Coleus) belonging to family Lamiaceae, with a many of traditional medicinal uses in India. C. forskolii is only known source of forskolin; a compound with a many uses in pharmaceutical industries. C. forskolii was lack in Egyptian flora. Moreover, there were no previously studies on this plant in Egypt. Therefore, the present study used tool of biotechnology to conserve the stocks of this plant by micropropagation. C. forskolii seedlings came from its native Thailand at June 2013 and were put in the greenhouse in Desert Research Center for creating an efficient micropropagation protocol. The study was carried out on the effect of growth regulators (cytokinins and auxins) on different micropropagation stages of the explants. In multiplication stage, initiated shoots were cultured on MS medium supplemented with various concentrations (0.5, 1.0, 1.5 and 2.0 mg/L) of cytokinins (6-benzylaminopurine (BA), Kinetin ( KIN) and Thidiazuron (TDZ). The mean number of axillary shoots per explant of C. forskolii reached the highest value 6.19 ±0.573 on MS medium containing 2.0mg/L TDZ. Where, the highest value of mean length was 6.44± 0.310 cm on MS medium containing 1.0 mg/L KIN. The mean number of roots / explant of C. forskolii reached the highest value and the mean length were 30.00 ± 0.577 and 11.8±0.860 cm respectively, on 1/2 MS medium containing 0.5mg/L indole-3-butyric acid(IBA). While, the highest value of shoot length was 11.8±0.860 cm on 1/2 MS medium containing 2.0mg/L naphthalene acetic acid (NAA). A percentage of 83% of rooted plantlets were successfully acclimatized after four weeks and grown normally in the greenhouse in sterile soil mixture of garden soil, vermiculate and sand (2:1:1/v/v/v). The protocol could be cost effective and useful in germplasm conservation and delivery of tissue cultured Coleus plants.
Afficher plus [+] Moins [-]ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS OF CLOVE AND THYME Texte intégral
2019
A., AbdelـFattah | Yasmin Aboelazab | M. Khallaf | Y. El-Kenany
This study was carried out to evaluate the antimicrobial activity of clove (Syzygium aromaticum) and thyme (Thymus vulgaris) ethanolic extracts. Different concentrations of ethanolic extracts were prepared and antimicrobial activity was determined against some bacterial, yeast and mold strains. The antimicrobial activity of examined extracts at different concentrations was determined against different cell population of tested strains and expressed as diameter of inhibition zone. Results showed that all clove and thyme ethanolic extracts were markedly inhibited the growth of all tested strains, however, the inhibition effect was differed with regard to the concentration of ethanolic extract as well as the type of tested microorganism. Generally, clove ethanolic extract had stronger antimicrobial activity rather than that of thyme ethanolic extract. Subsequently, clove and thyme are an extremely strong antimicrobial agent with usefull potential applications in food processing as a natural safe unsted of synthetic antimicrobial agents.
Afficher plus [+] Moins [-]ECONOMIC EFFICIENCY OF AGRICULTURAL LABOR WITHIN THE DETERMINATES USE OF MODERN TECHNOLOGY FOR WHEAT IN EGYPTIAN Texte intégral
2019
Mona Abdel-Naby | M. Rehan | M. Nasr
The Egyptian agriculture relies on technology to develop the productivity of most of the main crops, including cereal crops, especially wheat, which is reflected in increasing of the cultivated area. According to the Sustainable Development Strategy (SDS): Egypt Vision 2030, The cultivated area with wheat had reached about 1326 thousand feddan in 1980, then it continued to increase until it reached about 1955, and2716 thousand feddan in 1990, and 2007 respectively, and reached its maximum in 2016 by about 3351 thousand feddan. Also, the productivity duplicated from about 1.36 tons/fed. in 1980, and reached about 4..2 tones/fed. in 2016. Egypt plans to reach the area cultivated with wheat to approximately 4.2 million feddan and about 3.6 tons/fed of its productivity in 2030, in order to increase Autarky to about 81% from the locally production. To achieve this target, the absorption of technology must be further increased by increasing the different technology methods, which is reflected in productivity increasing. Wheat crop was selected for the field study through the availability of field data to estimate some economic and statistical indicators that are difficult and it may be impossible to use the secondary data in its estimation. Also, The governorate of El-Behaira was selected According to the relative importance of wheat-cultivated area,where as total area is about 378 thousand feddan representing about 11.27% of total Egyptian cultivated area, it also selected according to the availability of different types of machines which existing in that region. The study aims at estimate the economic efficiency of agricultural labor use in the light of specific modern technology which is used in Egyptian agriculture, represented by agricultural mechanization technology from the production farms of wheat crop in El-Behaira governorate during the agricultural season (2015-2016) in the short and long run of wheat farmers, where a simple random sample was selected from three villages in El-Behaira governorate (Berijat, Damshli, Alqam), The sample units divided three categories: the first category (less than 0.5 feddan), the second category (0.5 feddan - feddan), the third category (feddan and more) Short and long run for each category, in order to estimate some indicators of production and economic efficiency, and economics of scale. The estimation of short-run production function shows that total elasticity is about 1.3, 1.3, and 1.06 for the first, second and third categories of the sample respectively, while total elasticity is about 1.1 in the long-run, which reflecting the increasing returns to scale and farmers are producing in the first phase of the law of diminishing returns. The estimate of the cost functions in the short-run shows that the optimal production is about 14.1, 36.4 and 88.3 ardabb respectively for the three categories in the study sample, and the optimal production is about 47.7 ardabb in the long-run.
Afficher plus [+] Moins [-]SIMULTANEOUS EQUATIONS MODEL FOR LABOR MARKET IN THE EGYPTIAN AGRICULTURAL SECTOR Texte intégral
2019
Rania Youssef | Fatma Fahmy | Mona Ryad
The problem of research is the low and low wages in the agricultural sector, which makes it an employment sector, indicating that there is imbalance in the structure of the labor market in the agricultural sector, and thus the current deviation of agricultural employment from the optimum level that achieves economic efficiency, hence the main objective of The research is to assess the equilibrium situation of the labor market in the Egyptian agricultural sector, and determine the most important factors affecting it, through the study of both sides of the demand and supply of agricultural labor through Simultaneous Equations Model. The most important results obtained were: 1 - For the function of demand for agricultural labor: It was found from the estimated equation that the increase in the wages of the agricultural worker by one pound leads to a significant statistical decrease at a significant level of 5% in the demand for agricultural labor by about 135 thousand workers, as shown from the estimated equation Effect of production value Agricultural demand for agricultural employment amounted to about 29.7 thousand workers million workers. The impact of the supply of agricultural labor in the demand for agricultural labor, which reached about 645 thousand workers, was also shown. 2- For the agricultural labor supply function, the estimated equation shows that increasing demand for agricultural labor leads to an increase in the supply of agricultural labor by 1 million and 9,000 workers. It is also shown from the estimated equation that increasing the wage of the agricultural worker by one pound leads to an increase in the supply of agricultural labor by about 39 thousand workers. 3- As for the equilibrium situation of the labor market in the Egyptian agricultural sector, it was found that the wage of the agricultural worker amounted to about 43.338 thousand pounds / year, ie about 118.73 pounds / day, which is different from the actual situation of the wage of the agricultural worker, which averaged 26.164 thousand pounds / year Ie about 71.68 pounds / day as an average for the last three years. This means that the wage of the worker in the agricultural sector is less than the equivalent of 47.05 pounds per day. In addition, the total number of agricultural workers in the agricultural sector reached about 6.34 million workers, which differs from the actual situation of the supply of agricultural labor which reached 7.780 million workers as an average for the last three years. This means increasing the supply of agricultural labor to the balanced size of agricultural labor by (1.44 million workers), which explains the low wages in the agricultural sector, in order to load the agriculture sector with a larger number of workers than is supposed to be and hence the low wage in the agricultural sector and thus become an employment sector that will negatively affect the attractiveness of investments in the agricultural sector Which represents a sector Wide in the Egyptian economy is not negligible.
Afficher plus [+] Moins [-]ESTIMATE OF SOME GENETIC PARAMETERS FOR SOME VEGETATIVE AND YIELDING TRAITS IN COMPLETE DIALLEL CROSSES AMONG FIVE INBRED LINES OF CUCUMBER Texte intégral
2019
O. Gad Alla | S. El-Miniawy | S. Abd El-Hady | I. El-Oksh
This experiment was implemented in the experimental farm of (Misr hytech seed co. Giza, Egypt) during winter growing seasons 2014 and 2015 to evaluate the inheritance of some cucumber vegetative and yielding traits using a complete diallel cross among five inbred lines. The results showed that the mean square of genotypes, parental inbred lines, crosses and parent vs crosses were significant for the studied traits. The hybrids P2xP4 and P2xP5 exhibited a useful heterosis relative to Mp and Bp for the fruit yield per plant and number of leaves per plant. The mean square of general and specific combining ability was significant for the studied traits. The higher GCA/SCA ratio than the unity of the studied traits indicating to the greatest role of the additive gene action in the expression of these traits. The inbred lines P1 and P 2 exhibited significant positive i g ˆ effects in the traits of number of fruit per plant and fruit yield per plant, so these inbred lines could be act as a good combiner for developing high yielding genotypes. The F1 hybrids P1xP3, P2xP4, P2xP5 and P4xP5 as well as the reciprocal hybrids P4xP1, P4xP2, P4xP3 and P5xP3 recorded a significant positive ij S ^ effect but the reciprocal hybrids recorded a significant rij effect. The results revealed to more than one hybrid distinct in some traits that could be utilized in the greenhouse cucumber cultivation and the future breeding programs.
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