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MICROPROPAGATION AND EVALUATION OF GENETIC STABILITY OF FOXGLOVE TREE (Paulownia tomentosa)
2018
K. Hassan | A. Hosni | M. Hewidy | A. Abd El razik
This reasearch work aimed to fine-tune micropropagation of Paulownia tomentosa in addition to assessing the genetic stability of in vitro raised clones from it. Paulownia tomentosa explants were surface sterilized using clorox (commercial bleach 5.25% sodium hypochlorite) at 10, 20, 25 and 30% + 0.5 g/l mercuric chloride (HgCl2) at different duration times, i.e. 10, 15, 20 and 25 min. In the multiplication stage, shoots were transferred to MS medium at ¾ strength containing BAP and Kin each at (0, 0.5, 1, 2, and 4 mg/l). Whereas, the rooting medium was MS medium at ¾ strength with IBA and NAA treatments each at 0, 0.5, 1, 2, and 4 mg/l. Sterilized explant with 30% Clorox for 20 min recorded highest survival percentage. The treatment of Kin at 4 mg/l gave higher significant shoot length. Whereas BAP application at 2 and 4 mg/l gave highest significant value of both shoot number and leaf number. Both IBA and NAA at 0.5 or 1 mg/l gave highest significant root number/shoot. Whereas, auxin at 4 mg/l gave highest significant root lengths. Young plantlets resulted from in vitro were acclimitized successfully in a mixture of peat moss: perlit (2: 1) by volume that showed 85.93% survival. The genetic stability of in vitro raised Paulownia tomentosa clones was assessed by using intersimple sequence repeats (ISSRs) markers. All of the three ISSR primers screened, produced clear, reproducible and scorable bands. The molecular size of Polymerase Chain reaction (PCR) products generated 22 fragments by these ISSR ranged from ≈460 to18660 bp. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant, indicating 100% similarity. This confirmed the true to type nature of the in vitro raised clones.
Afficher plus [+] Moins [-]ESTIMATED GENE EXPRESSION IN MEAT QUALITY TRAIT UNDER STRESS CONDITIONS FOR TWO BROILER STRAINS
2018
Kareman Nasser | lamiaa Radwan | M. Mahrous | Neima Alsenosy | A. Farag
The objective of this study was to estimated gene expression in breast muscles of two broiler strains with used to Myoustatin gene in marketing age (5 weeks of age) under heat stress and compared to control group for 1 hour daily for 7 days in Shalakan farm of Faculty of Agriculture, Ain Shams University, broiler chicks (150 chicks in each strains) of 1 day of age were used in this study. So, the measurements were taken (respiratory rate, rectal temperature and estimated gene expression). The results obtained the gene expression of Myostatin gene in muscle breast was observed highest in Ross strain compared with Indian River strain under heat stress, but no different between strains ( Ross and Indian River) in control group. However, respiratory rate of Ross and Indian River under heat at 5 week of age showed that the Indian River strain was high significant at 5 week of age compared to Ross ones. As shown that rectal temperature of Ross and Indian River were a high significant effect of treatment (heat group was higher from control group) and not a significant of strain.The present experiment was conducted to estimated gene expression on meat quality traits of different tropical stress conditions in different broiler strains during 7 days and 5 weeks of age. Introgressions some major genes likes myostatin gene in muscle breast into broiler improve under the heat stress.
Afficher plus [+] Moins [-]IMPACT OF Allium sativum AGAINST Enterobacter sp. AS WATER BORNE PATHGENIC BACTERIA ISOLATED FROM RIVER NILE
2018
Rasha Ebrahim | Rawia Gamal | Samah Mohamed | R. Abdel-Rahman
To explore the antibacterial activities of Allium sativium (garlic) extract was tested against two waterborne pathogenic strains isolated from River Nile, to mitigate the increase of bacterial resistance to conventional antibiotics. The two isolates were identified as Enterobacter cloacae DSM 3264 BRB & Enterobacter cloacae MB11506_1CHB by MALDI-Tof-MS. Aqueous, methanolic and oil extraction of garlic were tested for their inhibitory activity against the selected strains using well diffusion method. Enterobacter sp. were more sensitive towards oil extract with inhibition zone 2.3 cm rather than aqueous and methanolic extractions with was 50%. Analysis of garlic essential oil by GC-MS dedicated six sulfur compounds represented 25% of total detected compounds in the oil.
Afficher plus [+] Moins [-]EFFECT OF SUBSTITUTION OF WHEAT FLOUR WITH QUINOA FLOUR ON QUALITY OF PAN BREAD AND BISCUIT
2018
E. Moawad | I. Rizk | Y. Kishk | M. Youssif
This study was carried out to investigate the effect of partial substitution (5, 10 and15%) of wheat flour (72% ext.) by whole meal quinoa flour (QF) on quality parameter of pan bread. QF contained the highest percentage of protein, Lipids, ash and crude fiber. Also, QF contained the highest amount of essential amino acids such as (threonine, methionine, lysine and histidine). From the results, it could be seen that, water absorption and degree of softening increased by increasing the substitution levels, but stability, resistance to extensions and energy of dough decreased. The addition of QF adversely affected on the specific volume of pan bread. The lightness (L*) and yellowness (b*) of pan bread decreased, but redness (a*) increased gradually by increasing QF. Evaluation of the organoleptic properties of pan bread revealed that no significant differences (P≥0.05) between control sample and bread samples contained QF for taste and summitry form. The bread contained QF had higher score for crust color, pore size and overall acceptability than control sample. Hardness (g) of pan bread increased and springiness decreased gradually during storage of bread at (25˚c ±2). The rate of staling of bread contained QF lower than control sample. Gluten – free biscuit made from 100%QF contained the highest percentage of protein, lipids, ash and crude fiber compared to that of corn and rice – quinoa composite flour. The addition of corn and rice flour adversely affected on the thickness, diameter and spread ratio. The highest spread ratio was noticed in the biscuit made from 100% QF. It is worth mentioning that the biscuit made from 100 % QF or that of corn and rice – quinoa composite flour gave the biscuit with sensory acceptable. On the other hand, it could be noticed to that the biscuit contained high level of quinoa flour was darker in compared to another samples.
Afficher plus [+] Moins [-]ECONOMETRIC MODEL OF DETERMINANTS OF THE EGYPTIAN WATER RESOURCES
2018
R. El-Sayed | A. AbdelMaksoud | Y. Arafa
forts to try to overcome all obstacles to water supply in the short and long run. Especially in the light of contemporary changes, especially the construction of the Al-Nahda Dam, and therefore the aim of the research to identify the most important determinants of water resources in Egypt, in preparation for inclusion in a proposed mechanism for the promotion of water resources in Egypt. The research aimed at analyzing the economics of water resources in Egyptian agriculture to identify the most important economic determinants facing the Egyptian water resources sector in general and the water uses in Egyptian agriculture in particular, as well as the most important strengths, weaknesses, opportunities and threats facing the Egyptian water sector. The results of the statistical assessment of the econometric model of the Egyptian water resource determinants under the influence of water policies show that the supply of available water resources is increasing by about 210 million cubic meters due to the Egyptian government adopting water policies aimed at rationalizing the use of water in all aspects of life, The repercussions of the construction of the Al-Nahda Dam have been in recent times. The most important of these policies is the reduction of cultivated areas of water crops, the development of plant varieties and varieties of strategic crops with lower water consumption rates, the expansion of the application of wastewater treatment technology, the expansion and adoption of modern irrigation techniques, and the lining and clearing of canals, waterways and other policies. Especially in recent times.
Afficher plus [+] Moins [-]EFFECT OF BENZYLADENINE ON MICROPROPAGATION OF BANANA SHOOTS TIP
2018
Sara Abdel-Motagaly | Yasmin Abdellatif, | H. Manaf | I. Ibrahim
The effect of benzyl adenine (BA) at 0, 2, 4, 6 and 8 mg l-1 on micropropagation of banana shoot tips was studied. This study also included the morphological responses of banana shoot tips especially with 0 & 6 mg l-1 of BA treatments in relation to some biochemical compositions (total soluble phenols, free amino acids, total soluble sugars, chlorophyll a, b and carotenoids). Growth in 6 mg l-1 of BA resulted in increase in the most morphological parameters compared to the rest treatments. The results showed that 6 mg l-1 of BA treatment significantly increased fresh and dry weights, number of shootlets, shootlet and root length and number of leaves and roots/plantlet as compared without BA. Accumulation of total soluble sugars, free amino acids and chlorophylls was enhanced by 6 mg l-1 of BA while the reverse was true with the rest biochemical compositions (total soluble phenols and carotenoids). The biochemical status and BA treatment at 6 mg l-1 during micropropagation of shoot tips in banana may be important for the development and optimization of strategies for large scale propagation and germplasm conservation.
Afficher plus [+] Moins [-]RURAL WOMEN'S CONTRIBUTION IN FOOD AVAILABILITY: A CASE STUDY IN KAFR TESFA VILLAGE, QALUBIA GOVERNORATE
2018
Doaa Khaleil | I. Rihan | M. Yehia | Samia Mahrose
The resent study aims to identify the score of rural women's contribution in food availability for her family through determining the nature of participation of rural women in various agricultural activities (plant and animal activities). Furthermore to determine the most important factors affecting rural women's contribution in food availability of the study sample. Finally, to identify the most important problems and obstacles facing the rural woman and limiting her contribution in food availability for her family. A study was conducted in "Kafr Tesfa" village, Kafr Shokr district in Qalubia governorate. A systematic random sample was selected including170 rural women from the village. A questionnaire was used during personal interviews with the rural women from December 2017 to January 2018. The data was tabulated and analyzed by using several statistical methods such as: range, arithmetic mean, mode, standard division, Spearman's rank correlation coefficient, Chi Square test and Kramer's V coefficient using SPSS program. The study results indicated that the score of rural women's contribution in food availability indicator ranges between (25) - (97) scores with Arithmetic mean (65.4) scores, standard deviation (19.99) score. The indicator range was divided into 3 equal categories. The results also showed that (45.3%) from the total population sample fell in the high category of the indicator. The medium category included (24.1%), and the lowest category included (30.6%) from the total sample population. To measure the association relationships between the score of rural women's contribution in food availability (dependent variable) and the study independent variables, the study used Chi Square test. Where, the study results indicated that there are association relationships between the dependent variable and the following independent variables: woman's social status, ownership of agricultural machineries, sources of access to food information at significance level 0.01. While the association relationships of variables: socio-economic level of respondent's family and get a pension were significance at level 0.05. To show the combined effect of the studied independent variables on a score of rural women's contribution in food availability (dependent variable) the study used Kramer's V coefficient for the strength of the relationship. The analysis of the data using Kramer's V coefficient showed that the Determination Coefficient was (0.560). This result means that five factors out of all independent factors explain (56%) from the total variance in the score of rural women's contribution in food availability (dependent variable) at probability significance level 0.01.
Afficher plus [+] Moins [-]INTERREGIONAL TRADE OF ARAB COUNTRIES
2018
M. Abdullah | M. Rajab | A. Alkhashan
This research aims to study the possibilities or opportunities for developing intra-Arab agricultural trade in order to achieve Arab integration through studying Arab agricultural trade in terms of value development, the extent of export coverage of imports and the structure of trade for various Arab countries. The Arab countries rely heavily on nonArab countries to fill this gap. It shows the low volume of trade in Arab inter-Arab agriculture and the increasing dependence of Arab countries on nonArab countries to obtain it. However, despite the large scale of these efforts, the volume of intraArab agricultural trade and Arab integration has remained low In light of the current circumstances, the economies of the Arab countries - as developing countries - face a new economic environment characterized by changes in economic structures and international relations. Therefore, the study of the conditions of Arab agricultural, agricultural and intra-agricultural trade and the impact of contemporary international variables should be explained. For how to achieve Arab agricultural integration in light of the possibilities available, and the variables existing in the international arena.
Afficher plus [+] Moins [-]EFFECT OF SOLAR DRYING ON THE QUALITY OF CORN SEEDS
2018
Gehad Abdalgawad | M. Abdel-Salam | Magda Mosa | M. Mostafa
The main objective of this investigation was carried out to study the ability of utilizing greenhouse solar dryers for drying of corn in order to obtain the best quality of dried grains for using it as seeds with the least drying time, and comparing with the natural sun drying method. Thus, contributing to increase the productivity. Corn cv. (Giza 168) was used for the experimental work at initial moisture content of 31.73% on dry basis (d.b). Two different drying methods of corn were tested for drying of ear and shelled corn. The two methods were natural sun drying and solar drying using greenhouse type solar dryers at different air velocities (0.5, 1.0 and 1.5 m/s). The experiments were carried out in rice mechanization center at Meet El- Dyba, Kafr El-Sheikh Governorate, Egypt during September 2016. Experiment included the flowing variables Two different drying methods (Solar drying method using greenhouse type solar dryer and natural sun drying method). Two different conditions of corn (complete ear-shelled corn). Three different air velocity (0.5, 1.0 and 1.5 m/s). High moisture ear and shelled corn was dried by using solar energy for heating air inside a greenhouse and compared with natural sun drying method. The results of quality tests that included standard germination test, vigor test and tetrazolium test for both drying methods for ear and shelled corn recorded high percentages for all treatments except solar drying of ear corn at air velocities 0.5 and 1.0 m/s. The average air temperature inside the solar dryer at air velocities 0.5 and 1.0 m/s reached to 43.9 and 42.4 ˚C respectively. High temperatures killed the germ of corn so the quality tests were reduced. Germination percentage of natural sun dried ear corn was 97 % and for solar dried samples at air velocities 0.5, 1.0 and 1.5 m/s was 79, 81, and 89%, respectively. The corresponding values for shelled corn were 93, 97 and 98 %.and 95 % for natural sun dried ones. The vigor test of dried ear corn recorded 90 for natural sun drying method and 34, 45, and 66% for solar drying at air velocities of 0.5, 1.0 and 1.5 m/s, respectively. The corresponding values for shelled corn were 86, 96 and 97% respectively, and 82 for natural sun dried samples. The recorded drying times were 26, 24 and 28 hours to reduce the moisture content from an initial level of 31.73 to final level of 14.07% (d.b.) for ear corn and for shelled corn were 20, 16 and 24 hours to reduce the moisture content from an initial level of an initial level of 31.73 to final level of 14.07% (d.b.) for ear corn and for shelled corn were 20, 16 and 24 hours to reduce the moisture content from an initial level of 27.23% to 14.12% compared with 46 and 38 hours for ear and shelled corn dried by natural sun drying method. Hourly costs of ear corn drying were 0.95, 0.88, 1.00 and 1.03 L.E/ kg for solar drying at air velocities 0.5, 1.0 , 1.5m/s and natural sun drying respectively. The corresponding values for shelled corn were 0.74, 0.58, 0.89 and 0.96 L.E/ kg.
Afficher plus [+] Moins [-]EFFECT OF SOWING MEDIUM AND NITROGEN FERTILIZATION RATES ON PRODUCTION AND GROWTH OF MAHOGANY (SWEITINIA MACROPHYLLA) SEEDLINGS
2018
This study was carried out at the nursery of Horticulture Department, Faculty of Agriculture, Ain Shames University during two seasons of 2015 and 2016 to study the effect of sowing medium and fertilization rates on vegetative growth and chemical composition of Swietenia macrophylla seedlings. Three different sowing media were used i.e., S M1= perlite: peat moss: compost (1:1:1), SM2 = perlite: peat moss: compost (1:2:1) and SM3 = perlite: peat moss: compost (1:1:2) (v/v). Also, four fertilization rates were used as follow: without fertilizer (control), 5, 10 and 15 g /plant of hydrocomplex fertilizer .The following data were recorded, stem length (cm), stem diameter (cm), number of leaves / plant, leaf area (cm2) and total fresh and dry biomass (gm). Moreover, chlorophyll a & b leaves contents (mg/g FW.), total carbohydrate content (mg/g DW), nitrogen, phosphorus and potassium content in leaves (% D.W.) were determined. Regarding the effect of sowing medium, the obtained results showed that, using sowing medium which content the highest percentage of compost (1:1:2) significantly increased stem length, stem diameter, number of leaves/ plant, leaf area, total fresh and dry biomass, total carbohydrate content and potassium leaves content in comparison the other growing medium while, the differences were insignificant in chlorophyll a & b, nitrogen and phosphorus leaves content. Concerning the effect of fertilization rates, using fertilization at the rate of 15g/plant caused significantly increased stem length, stem diameter , number of leaves / plant, leaf area, total fresh and dry biomass, chlorophyll a & b content, total carbohydrate content, nitrogen, phosphorus and potassium leaves content in comparison the other fertilization rates with the exception of leaf area, chlorophyll a , phosphorus and potassium leaves content at the rate of 10g/plant in the second seasons only. As for the interaction between sowing medium and fertilization rates, In general, application of growing medium which contain thehigh ratio of compost (1:1:2) was superior in all tested characteristics followed by which contain the high ratio of peat moss (1:2:1)at 15 gm fertilizationas compared to the other treatments.
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