Four different date palm male types were chosen to evaluate which male could be recommended to use as a pollinator for Barhi date palm cv. grown in Egypt. Three male types namely Ghanamy, Fard and Boyr were grown in the Fruit Trees Experimental Station at Dibba, Fojaira, UAE, and the fourth male was an individual Sewy satellite seedling grown in Egypt. Number of spathe/palm; spathe weight, length, & width ; pollen grains weight / spathe ; average of strand length as well as pollen grains germination % were determined for each male date palm tree from each male type . Moreover, pollen grains from each male type were used as a pollinator to evaluate their effect on fruit set and fruit quality of Barhi date palm cv. This study was carried out during both 2004&2005 seasons. Data showed that both Ghanamy and Fard male type were superior in their morphological characters as compared with Boyr or Sewy satellite seedling male type. Boyr male type showed the lowest values of morphological characters. On the other hand, there were no great differences in pollen grains germination percentage. Moreover, the results clearly indicated that Ghanamy pollen recorded the highest significant fruit set percentage and bunch weight (Kg) in both seasons of study. While, Fard pollen produced the highest fruit weight (g), length, diameter, flesh weight& thickness and flesh %. Boyr and Sewy pollen showed the highest ratio of fruit length / diameter in both seasons. Data also revealed that dates that were picked from bunches pollinated with Fard pollen had the highest total soluble salts (T.S.S) value and the lowest tannins content.

Lake Manzala in Egypt have shown great changes in water properties including pH (7.4 to 8.4), total suspended solids (19 to 350 mg/l), total dissolved salts (1578 to 33480 mg/l), dissolved oxygen (2.25 to 8.45 mg/l), ammonia (0.00 to 44.8 mg/l) and nitrate (0.00 to 8.96 mg/l). In addition there has been quite high bacterial density in water and fish of the lake, including total viable bacterial counts and indicator organisms such as total coliforms, faecal coliforms and some other organisms such as Aeromonas spp., Vibrio spp. and Staphylococcus spp. However, the magnitude number of bacteria in water and fish was found to vary sometimes considerably and sometimes slightly depending on the site of sampling. Our results herein showed that LakeManzala is highly contaminated.

A total of 174 actinomycete cultures were isolated from a constructed biological water treatment system (BIOWATSYST)established at Abu Attwa station in Ismailia city, Egypt and funded by the European Commission Grant No.IC18-CT97-0163. The isolates were identified to belong to eight genera; Nocardia, Streptomyces, Intrasporangium, Micromonospora, Nocardioides, Actinomadura, Nocardiopsis, and Thermomonospora.  They were screened for their antibacterial, antifungal and cytotoxic activities against certain human and plant pathogens. Antimicrobial activities were determined by measuring bacterial and fungal growth inhibitions while cytotoxic activity was studied by using the Artemia salina bioassay. Thirty two percent of isolated cultures displayed antibacterial activity, 15% displayed antifungal activity and 9% displayed cytotoxic activity. Members of genus Streptomyces has recorded as the most frequent active isolates against tested bacteria (42%) and fungi (49%). However, the most cytotoxic activity was found with members of genus Nocardia (46%).  Results evaluated the fact that actinomycetes isolated from such systems could be considered as promising source for antimicrobial and cytotoxic bioactive agents.

This study was conducted for comparing three slow release N fertilizers namely, sulphur-coated urea (SCU), phosphorus – coated urea (PCU) and Enciabene with the conventional fast release N fertilizer namely ammonium nitrate each at the recommended rate of N i.e., 1000 g N/tree/year for growth characters on the three growth flushes, percentages of leaf N, P and K in the Spring growth cycle, date of first bloom, number of panicles/tree, fruit retention %, yield as well as physical and chemical characters of fruits in two mango cvs Figri Kelan and Keitt during 2004 and 2005 seasons. Results showed that suppling the trees of both mango cvs with the three slow release N fertilizers were superior to the application of the fast one in improving shoot length, number of leaves / shoot and leaf area in the three growth cycles, percentages of leaf N, number of panicles / tree as well as physical and chemical properies of the fruits. The slow release N fertilizers slightly advanced first bloom date. Application of sulphur-coated urea (SCU), phosphorus-coated urea (PCU) and Enciabene (ENC) in a descending order, was very favorable in this connection. These results were true for both mango cvs. It is suggested to fertilize the trees of both mango cvs Figri Kelan and Keitt with N at 1000g/tree/year as sulphur-coated urea fertilizer (2.4 Kg/tree/year) for obtaining an economical yield with fairly good fruit quality

Eight most currently used antibiotics were examined for their antibacterial properties against Gram-ve bacteria as Pseudomonas aeuroginosa, E. coli, Proteus vulgari and Gram+ve as Staphylococcus aureus, Streptococcus pneumonia. Nitrofurantoin was the most effective against the tested bacteria, the inhibition zones ranged between 16-20mm and the MIC between 65-85ug/ml followed by ampicillin (11-18mm), ciprofloxacin (9-12mm) and gentamicin (6-9mm). The erythromycin was the lowest effective against the tested bacteria. Also, seven volatile oils were applied by contact and fumigation methods to study their effect on the tested bacterial strains. The fumigation method gave the highest inhibitory effect more than contact method and the thyme oil gave maximum inhibitory action (inhibition zone 20-28mm) against all the tested bacteria, and the MIC ranged between 0.1-0.15mg/ml followed by marjoram oil (19-25mm) and the MIC between 0.1-0.2mg/ml, cinnamon oil (12-16mm) and the MIC between 0.2-0.3mg/ml. Anise and chamomile oils did not gave any response against all the tested bacteria. The combination between thyme and other tested oils gave a synergistic effect for inhibitory action against all the tested bacteria, if compared with thyme oil alone. The combination between thyme and marjoram oil gave the maximum inhibition zones (20-29mm), followed by thyme with cinnamon oil (20-27mm), thyme with geranium gave (18-27mm), thyme with peppermint (17-27mm), thyme with chamomile (16-27mm) and thyme with anise oil (15-26mm). The combination of thyme oil with different tested antibiotics gave the lowest inhibitory effect than combination between thyme and other volatile oils against all the tested bacteria. The protein and DNA content of treated bacteria with thyme oil were increased by 38.46-47.37% and 34.26-46.94% respectivily, if compared by non-treated bacteria

Surgical site infection (SSI) remains an important cause of morbidity and mortality among hospitalized patients. A total of 254 bacterial isolates were collected from 303 surgical specimens which were isolated from 92 males (53.2%) and 81 females (46.8%), their mean ages were 39.6±16.05 years (ranged from 1 month to 74 years). These isolates were identified as Staphylococcus aureus,Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonasaeruginosa and commensal Gram+ve and Gram-ve bacteria. The most effective antibiotics were imipenem while ampicillin and penicillin G (First and second generation of b-Lactam) showed much lower activity against all types of bacterial strains. The minimum inhibitory concentrations (MIC) of  imipenem determined by E-test were 0.047- 0.064 , 0.125, 0.19, 0.25 and 0.75mg/ml against Staphylococcus aureus, Escherichia  coli, Proteus vulgaris, Klebsiellapneumoniae  and Pseudomonas aeruginosa  respectively.

Plant growth and root nodules of infected Faba bean plants with broad bean mottle virus were significantly reduced in plant height, leaf area, dry matter, number and size of nodules in comparison with healthy ones. As well Leghaemoglobin content and Nitrogenase activity were reduced in infected nodules at 45 days after BBMV inoculation. The amount of N2-fixed as expressed by acetylene reduction was reduced considerably by 38.8%. Nitrogen content as an indicator for the efficiency of N2-fixation was reduced in shoots, roots and root nodules of infected nodulated plants as compared with healthy nodulated ones. Differences between bacteroids in healthy and in BBMV infected cells were no longer apparent. These differences include: (i) a decrease in endocytotic and exocytotic vesiculation on the membrane envelope of the bacteriods and on plasma membrane of bacteroidal cells, (ii) a decrease in number of vesicles in the space between the bacteriod and the membrane envelope. The possible significance of these changes relative to the decreased efficiency of N2-fixation was discussed.

The nine most frequent bacterial strains out of 127 were isolated form ten drinking water samples collected from tap water and bottled water in sharqia governorate. The nine isolates were purified and examined for their resistance to increasing concentrations of two heavy metal ions, lead (Pb+2) and iron (Fe+3). Four stains out of the nine isolates encoded I, II, IV, and VIII showed the highest efficiency of both Pb+2 and Fe +3 uptake from nutrient broth media containing 100 ppm of the heavy metal ions. The four bacteria were preliminary identified and then confirmed by the Biolog examination as Corynebacterium jeikeium, Pseudomonas putida biotype A, Acinetobacter calcoaceticus and Acidovorax delafieldii. The increased concentrations of Pb+2 ions (from 100 to 500 ppm) in nutrient broth media had deleterious effect on the process of heavy metal uptake (biosorption) by all the four selected isolates. Whereas percentage of Pb+2 uptake decreased from 42.9 to 24%; from 72.6 to 42%, from 78.9 to 37% and from 68.8 to 45%, for the four selected isolates, respectively. Meanwhile there was slight decrease change in Fe+3 uptakes percentage accompanying the increase in heavy metal ion concentration. Optimization of the cultural conditions releaved maximum uptake op pb+2 and Fe+3 by the four tested strains in presence of 100 ppm heavy metal concentration when incubated at 25oC except for Acinetobacter calcoaceticus at 35oC in case of Pb+2 uptake, when pH, was adjusted at 5 under static conditions. Upon addition of 50 ppm Cu+2 ions to broth media supplemented with 100 ppm Pb+2 ions, the percentage of metal biosorption by the four tested isolated decreased between 41.11% and 48.45% according to type of strain. Similarly presence of Cu+2 ions caused decrease in Fe+2 uptake by the four isolates ranging between 29.14-45.1%. Percentage of Pb+2 ions uptake by the tested bacteria sharply decreased when a sterile tap water sample was used as natural medium for cell-metal contact. The percentage of uptake inhibition of Pb+2 ranged between 57-65.23% and between 75.1-84.27% for Fe+3 ions. Cell hydrolysate of three tested bacteria appeared to be free from plasmid DNA proving that the genetic character of heavy metal resistance is plasmidless and related to chromosmal DNA in case of Cory. jeikeium, P. putida and A. delafieldii. On the other hand, Acinet. Calcoaceticus contained plasmid of size 23.130 kb. Examining Acientobacter calcoaceticus using transmission electron microscope revealed the accumulation of Pb+2 ions on bacterial cell surface and the intracellular absorption of Fe+3 ions.

This study was carried out to compare, on  the basis of some biochemical nature, five generations of backcrossing resulting from six crosses between  four varieties of durum wheat (M,  W,  S and P) as three crosses  MxW,  MxS, and PxW along with their reciprocals  WxM,  SxM, and WxP. A sufficient knowledge of the biochemical characteristics of the grain and their genetic transmissions, allows their use in selection.  These characteristics can be used as criteria for selection of seeds for more judicious orientation. From the obtained results, a relative variability appeared at the various varieties and their descendants for the studied parameters (the weight of thousand grains, content of protein, moisture content, the volume of sedimentation and capacity of hydration of the gluten). Generally, the studied samples are relatively rich in proteins.  The descendants of cross MxW have on average a significant volume of sedimentation, they can be used for wheat improving.  For the moisture content, cross MxW records the greatest value with a more significant descendants BC5.  With regard to the polymorphism of the glutenines, some bands were transmitted by the recurrent parent varieties used as female parents.

From infected pink and spiny bollworm larvae, collected from Qualyobia Gover-norate, Egypt, 13 bacterial isolates belonging to 7 species were detected. The effi-ciency of these bacterial isolates was evaluated on newly hatched pink and spiny bollworm larvae. Three bacterial species, namely, Pseudomonas viridiflava, Serratia grimesii and Cellulomonas flavigena had no efficiency. Meanwhile, four other en-tomopathogenic isolates bacterial species, Pseudomonas pyrrocinia (A1), Serratia marcesens (M3), Serratia rubidaea (E3) and Bacillus thuringiensis (S2) had notice-able efficiency. The efficiency of these isolates was compared to two commercial products, Dipel 2X and Protecto. Biochemical studies showed differences in total proteins bands patterns in uninfected and infected larvae.