A study was conducted to assess two enzyme detoxifying activities and insecticide-resistance gene expression quantitation using real-time (PCR). Four populations of pink bollworms were compared with the baseline laboratory strain. Field populations showed higher levels of enzymatic activity, glutathione-S-transferase and β–esterases, than those of the laboratory strain. The amplification curves scored a cycle threshold (Ct) value of 25 for the ribosomal protein subunit7 (rps7). For the BtR gene, the Ct values of analyzed biological groups ranged from 20 for the laboratory strain to 24 for the Fayoum group. For Cad1, the Ct values ranged from 19 for laboratory strain to 23 for Fayoum and Qalubia populations. Therefore, the present work introduces a method for the challenge of monitoring resistance to Bt toxins in crops which requires, according to the IPM (Integrated Pest Management) program, a wise insecticide application.

Aphid is a major insect pest attacking ornamental plants. It causes great damage by reducing the economic values of plants. The investigated research aims to determine the relation between leaf biochemical components, morphological structures of plant leaf surface and the susceptibility of seven ornamental plants to Aphid Infestation. Highest recorded aphid infestation was observed on Tecoma stans during 2017 and 2018 seasons followed by Rosa damascena and Jasminum grandiflorum. There was no rec-orded infestation on four inspected ornamental plants, Ruta graveolens, Schefflera actinophylla, Nerium oleander and Bougainvillea spectabilis. Activity of antioxidant enzymes plays as defense line when increasing the insect population density. Biochemical components are varied among different plants that prevents or reduces insect infestation, as tannins, total phenol, and Phenol oxidase. Trichomes are considered as main morphological character in plant defense system. These results enhanced utilization resistant ornamental plants due to preventing aphid infestation. It is considered one of the integrated pest management programs, that achieves health environment without more insecticidal application.

At the Experimental Farm, Agriculture Faculty, University of Ain Shams, Qaliobia Governorate, Egypt, in order to investigate the influence of biofertilizer ( Bio. ); Rhizobium ( R ), mycorrhiza ( MF ), R + MF and control, seaweed extract (SWE) concentration (zero, one, two and four gram per liter) and their interactions on vegetative growth, productivity and quality of seed common bean cv. Nebraska. Before planting, the seeds were soaked in MF (glomus sp.) and sown on the first of March 2016 and 2017 seasons respectively. The experimental plot area was seven m2 (four rows). The length of each row was 2.5 m and width of 0.7 m. The plant's distance was seven cm on one side, and an alley (one width m) was left as a border between the treatments. After two weeks of planting, young plant were inoculated with R phaseolus after the first irrigation. Plants were sprayed three times of SWE (30, 45 and 60 days) after sowing. A split plot design with four replicates was used; where the main plots are Bio treatments and the subplots are SWE treatments. The obtained results clearly indicated that different applied treatments increased measured growth characteristics (No. leaves/ plant, leaf area and total chlorophyll (SPDS)), the yield and components of yield (No. pods/plant, seed yield /plant and seed yield) and chemical constituents (total protein, carbohydrates, nitrogen and phosphorus) in seed after harvest. As well as, the best results were obtained by interactions between SWE (two and four gram per liter) and Bio. (R + MF) treatments in the two assigned seasons So adding combination between Bio. ( R + MF ) and SWE (two or four g) to improve vegetative growth, productivity and quality of common bean seed could be recommended under organic system ( conversion to organic agriculture ).

The frozen vegetable industry in Egypt is one of the most important industries that contribute to the provision of vegetables throughout the year to the consumer, and the Egyptian frozen vegetables value of exports of preserved food industries as the may reach $86.61 million on average for the period (2011-2015) with relative importance of 47.68%. The problem statement of research represent that the agricultural production in general and from vegetables and fruits in particular and the high percentage of lost and damaged, which threatens the development of Egyptian exports of these products if the export of Egyptian manufactured products does not keep pace with its international counterparts, Therefore, the research aimed to estimate the external demand for Egyptian frozen vegetables, and the determinants of that demand in the most important import markets of Egypt during the period (2007-2018). The research has reached some conclusions, including That Saudi Arabia, The United Arab Emirates, Jordan, the United States of America, Italy, and France are the most important countries importing Egyptian frozen vegetables, where the percentage of Egypt's exports of frozen vegetables to these countries amounted to about 57.1% of the total amount of Egyptian frozen vegetables exported during the period (2014-2018) Egypt's exports of frozen vegetables to these countries amounted to about 55.6% of the total value of Egyptian frozen vegetable exports for the same period. The high price elasticity of Egyptian frozen vegetables for the UAE market compared to the Saudi market with a value of about 1.5, 0.46 respectively, which means that the individual demand for frozen Egyptian vegetables for the Emirates is elastic, while the individual demand for frozen Egyptian vegetables for Saudi Arabia is inelastic. This reflects the importance of Egyptian frozen vegetables in the Saudi market and deal with them as a necessary commodity at a time when this importance is diminishing in the UAE market, where dealing with frozen Egyptian vegetables as a luxury goods. The same table also shows the increase cross elastisates of the Italian market by about 2.3 compared to the Saudi market, where the cross elasticity of those markets where 2.3, 1.3 respectively, which means that the degree of competition in the Italian market is higher than the rest of the markets. The italian market's income elasticity is also shown to be higher compared to the UAE and US market with a value of about 6.5, 2.2, 0.66 respectively. The methods of estimating the individual demand for Egyptian frozen vegetables using import statistics and using export statistics indicate different types of elastisates for the demand for Egyptian frozen vegetables for the Italian market and the Saudi and Emirati market from elastic demand to inelastic demand, which indicates the existence of Tri-Market Operation of Egyptian frozen vegetables are in these markets. The need to increase Egyptian efforts to revive the Egyptian frozen vegetable market in the UAE market as one of the most important export markets for these goods. Try to export directly to the countries which it is already exported by identifying the countries importing from Egypt to be an effective and real demand and not an indirect demand.

Under screen house conditions, two experiments were carried out to evaluate certain bacterium, Pseudomonas fluorescens isolates regarding reproductive potential of root-knot nematode, Meloidogyne incognita, infecting tomato or eggplant. Results on tomato revealed that, on the basis of average total percentages nematode reduction, the over topped results were gained with P. fluorescens (Pf2) which recorded the highest significant (P≤0.05) average nematode reduction (61.3%) and higher percentage reduction of females (77%) per plant. The second rank was obtained by Pf3 which reduced all nematode numbers as an average of 56.9%.On the basis of average total percentages plant growth and weight of fruit increases, four bacterial treatments can be ranked in a descending order as follows: Pf9 > Pf4>Pf1and Pf7, as they achieved the highest average total percentages increases of 96.0, 47.3, 38.2and 29.8%, respectively compared to other treatments and untreated check. Regarding to eggplant, the over topped results observed was achieved by P. fluorescens (Pf10) which recorded the highest average total nematode reduction (66.2%) with higher reduction of (J2s) in roots (89.9%) per plant and in soil (78.8%) per pot. The second rank was obtained by Pf9 and Pf2 where they reduced all nematode numbers as averages of 55.9% and 54.9%, respectively. Also Pseudomonas isolates enhanced the plant growth of eggplant, averages were found in a descending order as follows: Pf1 (20.0%), Pf9 (18.7%) and Pf10 (18.3%) . It is worthy to note that the most distinct growth criteria was fresh weight of roots as it achieved higher percentage increase (58.1%) by using Pf9 followed by Pf1 as it caused 40.6% increase compared to untreated check. The highest average percentage of fresh weight of shoot were recorded for Pf10 (26.4%) and Pf8 (22.1%). Whereas for dry weight Pf3 (29.8%) and Pf2 (19.1%). In conclusion, the tested biocontrol agent was efficient in controlling the root-knot nematode on tested plants.

The effect of Pink Pigmented Facultative Methylotrophic (PPFM) bacteria as a bio-stimulant on vegetative growth, macro-nutrient elements ( N,P and K ) contents , chemical constituents and volatile oil percentage and components in rosemary plant (Rosmarinus officinalis L.) were studied during 2018 and 2019 seasons. Plant height (cm), number of lateral branches/plant, herb fresh & dry weights and root fresh & dry weights of rosemary plant were all greatly affected with all applied treatments of PPFM when compared with the control. The hugest effect was recorded by 1% PPFM bacteria spraying in both studied seasons. However, no significant effect was recorded due to PPFM applied treatments on P%. Highest values of total carbohydrates were recorded by both 1 and 3%. PPFM bacteria spraying in both studied seasons. Whereas, the highest values of C/N ratios were obtained with 3% PPFM in first season and 1% PPFM in the second one. A substantial effect from PPFM bacteria spraying was observed through its effect on reducing the level of NO3 and NO2 in rosemary herb when compared with the control. In any case, 3% of PPFM bacteria spraying was more effective than other treatments in recording the least values of NO3 and NO2 in first season compared to the control. Volatile oil % was enormously increased with 3% PPFM bacteria spraying in both studied seasons than other treatments or control. The main components of volatile oil of rosemary plant were Camphor, Eucalyptol, Verbenone, Borneol, Pinene, Pinanone, Bornyl acetate and Camphene. The treatments of PPFM bacteria spraying immensely stimulated the component % in volatile oil of rosemary herb than in the control. It could be concluded that the application of PPFM bacteria as foliar spraying in rosemary plant production had many advantages especially reducing NO3 and NO2 levels and increasing volatile oil % and volatile oil fractions not to mention it increased the percent of active ingredients.

The present study aimed to produce function yoghurt drink fortified with different types of herbal extracts (Ginger, Amla, Curcuma) and assess its therapeutic effect in hepatitis rats. The rats were randomly divided into two main groups. The first group were control (-) (6 rats) was fed on a standard diet, while the second group (30 rats) were fed on standard diet and injected it by CCl4 "for two weeks" to induce chronic damage in the liver (hepatitis) then divided into five groups (6 rats each group), then treated by plain drinking yoghurt and different herbal drinking yoghurt fortified with different types of herbs extract. Different Biological attributes were determined. The antioxidant activity, total phenolic content and chemical properties were evaluated in herbal extracts and yoghurt product and their hepatoprotective effect of each alone evaluated in albino rats against carbon tetrachloride (CCL4) induced liver injury. The hepatoprotective activity was assessed using various biochemical parameters and histopathological studies. The results indicated that functional flavoured drinking yoghurt containing herbal extract exhibited no significant differences were observed in total solids, fat, protein, ash, and lactose content them control samples. Drinking yoghurt fortified with Curcuma exhibited highest content of antioxidant activity and total phenolic content among all treatments. Also, rats fed on diet supplemented with drinking yoghurt fortified with Curcuma for 28 days showed higher potential hepatoprotective effects compared to liver injury control group (IC). The rats succeeded to restore the biochemical parameters and improved the histological alteration of the liver. This improvement was pronounced partly in the group received drinking yoghurt fortified with Ginger and Amla herbal extracts while, the group received drinking yoghurt fortified with Curcuma herbal extract were improved totally. It could be concluded that drinking yoghurt fortified with different herbal extract especially Curcuma can be used as ingredients in functional foods for people suffering from liver diseases.

The problem of fish food security is represented in insufficient strategic stocks of fish, especially in light of the increasing demand for fish, and lower production than consumption. The research mainly targeted the study of the situation of fish food security in Egypt, in addition to a set of sub-goals, which are the most important that the estimation of both the strategic stock of fish and fish food security coefficient in Egypt, studying of the most important factors affecting fish food security, studying of future expectations for fish food security, This is in addition to studying the problems that Obstruct the continued increase in fish production. The research relied on achieving goals on the use of qualitative statistical methods and economic equations for estimating both strategic stocks and security coefficient for fish. As Quantitative statistical methods were used, which are represented in using the simple and multiple regression method to estimate the functions of consumption, production, import and export of Egyptian fish, as well as food security coefficient in order to determine the most important variables that affect it by building an econometric model that explains the determinants of food security of fish. The double logarithmic model was the best model used in terms of economic logic and statistical significance, and Autoregressive and Integrated Moving Average (ARIMA) model was used to forecast production, consumption, imports and food security coefficients for fish until 2025. In addition to using the statistical method, One-way Anova- Analysis of Variance, to test the differences between a number of independent groups that represent problems that impede the continued increase in fish production, as well as the use of the least significant difference method (L.S.D) to arrange the elements of each problem. By estimating the growth rate of fish production and consumption, the fish food gap and the average per capita share, it was found that the growth rate of each of them took an increasing general trend during the study period, where the annual growth rate is estimated at 5.4%, 4.9%, 2.8%, and 2.7% for each of them, respectively. It was also found that the quantity of the surplus in fish attained about 0.9 thousand tons on average during the study period, and this surplus is very small, not enough consumption per day. It is also clear that the fish food security coefficient reached about 0.00073 on average during the period (20002018) and this indicates a decrease in the fish food security rate. The forecast values of the fish food security coefficient have shown that the fish food security coefficient tends to decrease from about 0.00076 in 2019 to about 0.00073 in 2025. And by studying the most important variables that affect fish food security in Egypt through the three basic functions, which are the fish demand function, the fish supply function, and the fish food security coefficient function, of the Simultaneous model, according to the economic logic and statistical significance, Increase in both national income (x13), imports of fish (Y3t), local production of fish (Y1t) by 1%, the food security coefficient of fish increases by 0.04%, 0.102%, 0.49% for each of them respectively, and when increasing Consumption (Y2t) by 1%, the fish food security coefficient decreases by 0.67%. This has proven the significance of the variables at the level of significance 0.01, as it turns out that by increasing exports (Y4t) by 1%, the food security coefficient decreases by 0.01% and this decrease is statistically significant at the  level of significance 0.05. A stratified random sample was chosen according to the cognitive and functional level, with the problems and obstacles of continuation of increasing fish production in Egypt. The sample was of those interested in the field of fish wealth. The problems were identified and divided into five categories which are: environmental problems, financing and production problems, marketing problems, problems related to scientific research, administrative problems. The study resulted in several recommendations, the most important of which are: Increasing the expansion of culture operations in floating marine cages. Increasing investment orientation for fish production from its various sources and providing financial support to small fish producers. Close control of the various pollution image processes of natural resources, overfishing, offensive fishing, and fishing fry and limiting their spread to preserve biological stocks.

The objective of this study was to evaluate the genetic improvement in Zaraibi goats herd raised on El-serw research station located in the north eastern part of Nile Delta, Egypt. The station belongs to Animal Production Research Institute (APRI). Some body weights at different ages were included in genetic and environmental trends. These weights were 4-month weight (4M), 6-month weight (6M), and 12-month weight (12M). Data and pedigree information were collected from 1988 until 2018. The least square means of body weights at 4M, 6M and 12M were 12.14±0.02, 15.49±0.02 and 24.08±0.04 kg, respectively. Year of birth, season, gender of kid, parity of dam and type of birth significantly (P<0.01) affect growth traits. Genetic parameters were estimated using a multi-trait animal model program (MTDFREML). The same previous fixed effects were included in this analysis regarding the animal as random effects. The heritability of the studied body weights tend to increase as age increases. That is inversely matched with the environmental proportion of total variance which was found to be 0.72, 0.66 and 0.62 for M4, M6 and M12, respectively. The heritability value was estimated as 0.28 for 4M. Genetic trends were obtained as the regression of the predicted breeding values on years of birth and Environmental trends were obtained as the regression of year constants on years of birth. The annual genetic change trends were positive and significant for 4M, 6M and 12M (0.091, 0.121 and 0.158 kg/year, respectively) and the annual phenotypic change trends were (0.020, 0.045, 0.117 kg/year, respectively). Genetic trends for M12 trait were higher than those for other traits in this study. Genetic and environment correlations between 4 month body weight and other growth traits were positive. Negative environmental trends indicates poor management system for the flock during studied period. Weight at 12 months of age has the highest heritability in studied body weights and suggested to be selection criteria to improve yearling weight for Zaraibi goat.

The present work aimed to optimize amino acids production by Bacillus amyloliquefaciens 35s from chicken feather using response surface methodology (RSM) and applying the produced amino acids in biological control of powdery mildew disease. Variables affecting amino acids production from B. amyloliquefaciens 35s were screened using Plackett Burman design. Factors showed to be significant to produce amino acids were optimized using central composite design (CCD) of RSM at three coded levels (-1, 0, +1). All obtained data were analyzed by ANOVA with post hoc multiple comparison analysis performed using Tukey’s HSD. TGY (tryptone, glucose, yeast extract) was the basal medium. Impacts of medium components were studied using Plackett-Burman design. “Design Expert 12” StateEase was used to analyze the experimental Plackett- Burman design. Feather, yeast extract, CaCO3, KH2PO4 and agitation rate (using shake flask) were optimized statistically by the CCD design of the RSM. Validation of statistical model of the medium components was done by carrying out the experiment at optimum conditions of the process parameters as determined by the model. Amino acids types were identified by amino acid analyzer. Among the significant medium components, feather, yeast extract, CaCO3, KH2PO4 and agitation rate showed to have significant effect on amino acids production. Predicted maximum amino acids production was observed near the mid-point concentrations of the mentioned above factors and the experimental value (6.55 g/l) was very close to the predicted value of (6.60 g/l) predicted by the model. Amino acid analyzer analysis for the produced amino acids shows the release of asparagine, threonine, serine, glutamine, proline, glycine, alanine, cysteine, valine, methionine, isoleucine, leucine, tyrosine, phenyl alanine, histidine, lysine and arginine, respectively. Effect of chicken feather hydrolysate on cucumber powdery mildew disease was tested by applying foliar application with different concentrations (0, 5, 10, 15, and 20% v/v), then degree of infection and sporulation on cucumber plants were assessed. The lowest concentration of chicken feather hydrolysate showed the lowest effect on disease index. Conversely, cucumber resistance increased by the increasing of feather’s hydrolysate concentrations associated with a clear reduction in the disease index and sporulation. Aside from, extra foliar application showed a decrease in the disease index for all concentrations compared with untreated plants. Foliar application with 20% concentration of chicken feather hydrolysate had the highest stimulation of the studied oxidative enzymes i.e., peroxidase (POX), phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) and superoxide dismutase (SOD).