The objective of the study was to examine the physicochemical properties and emulsification stability of three different Auricularia auricula polysaccharides (AAP) obtained through hot water extraction (AAP-W), hot acid extraction (AAP-A), and hot alkaline extraction (AAP-AL), respectively. The findings indicated that AAP-W exhibited superior emulsification stability compared to the other two polysaccharides. AAP-W was employed as a natural emulsifier for emulsion preparation, to examine the influence of varying polysaccharide concentrations and oil-water ratios on emulsion stability. Additionally, an investigation was conducted into the stability of the emulsions with respect to pH and salt ion concentration. The findings revealed that the most favorable polysaccharide concentration for the AAP-W emulsion was determined to be 1%, while the volume fraction of the oil phase was established at 0.5. It was also observed that the emulsion exhibited robust stability even in challenging conditions characterized by strong acidic (pH 3−5) or basic environments (pH 9−11), as well as high concentrations of salt ions (0−500 mM). Furthermore, the construction of an AAP-W emulsion system incorporating β-carotene was undertaken to enhance the preservation, bioavailability, and digestive stability of β-carotene, thereby expanding the potential applications of Auricularia auricula polysaccharides. This endeavor also presents a novel approach towards the advancement of novel functional food products.

To swiftly and noninvasively assess the freshness of bighead carp heads within simulated cold chain environments, an excitation-emission matrix fluorescence spectroscopy coupled with a long short-term memory network (EEM-LSTM) model was developed. Through the parallel factor algorithm based on analysis of residuals, diagnosis of core consistency, and split-half evaluation, three key fluorescent components from fish fillets were extracted, with the most significant components linked to tryptophan and NADH, both indicative of fish freshness. The EEM-LSTM model exhibited coherent trends in freshness indicators and demonstrated exceptional predictive capabilities for four freshness indicators simultaneously, achieving R2 values exceeding 0.8840 in simulated cold chain situations. Relative errors in the supermarket direct sales cold chain were less than 10%, surpassing those of the long-distance transport cold chain. Hence, the EEM-LSTM model stands validated for predicting fish freshness in simulated cold chains, holding promise for real-world aquatic product freshness forecasting within cold chain scenarios.

Baijiu Daqu, a traditional component in the Baijiu brewing process, serves as both a 'saccharifying fermenting agent' and an 'aroma-producing catalyst', embodying a rich historical legacy. Daqu offers a diverse microorganism environment that is crucial for the fermentation of Baijiu. The distinctive flavor profile, a key attribute of Baijiu, is intricately linked to the microflora present in Daqu. To date, research on Daqu has primarily concentrated on the diversity of microbial communities, microbial interactions, flavor characteristics, and biochemical properties. The functional enzyme system in Daqu serves as a crucial link connecting the flavor of Baijiu with the microbial community of Daqu. However, reviews that particularly focus on the role of enzymes in determining the quality of Daqu have not yet been reported. Thus, here the types and production processes of Daqu are initially summarized. Then, the pathways involved in the production of the major flavor substances in Daqu are elucidated, as well as the role and contribution of different functional enzymes in the formation of Daqu flavor. Finally, the current technologies for improving Daqu flavor through microbial inoculation aree discussed, including the advantages, shortcomings, and bottlenecks of microbial inoculation. The findings gained in this study provide valuable information for the efficient production of high-quality Daqu for the brewing of Baijiu.

Serum albumin can bind with a diverse range of small molecules. It could therefore serve a protective or carrier function, and effectively address the issue of anthocyanins' susceptibility to decomposition. The anisotropic effect of the magnetic field (MF) can influence their interaction, thereby playing a distinct role in molecular bonding. In this study, bovine serum albumin (BSA) and cyanidin-3-O-glucoside (C3G) were used as raw materials. The mechanism underlying the formation of BSA-C3G complexes induced by static magnetic field (SMF) was investigated through analyses of secondary structure, functional groups, dipole moment, crystal cell dimensions, and microstructural characteristics. BSA and C3G were treated with 50, 100, 150, and 200 mT, respectively. As the magnetic intensity increased, the secondary structure of the complex changed, the α-spiral content, β-corner content, and irregular curl content decreased, while, the β-folding content increased. The average grain size of the BSA-C3G composite was observed to decrease. Furthermore, alterations in the crystal cell dimensions of the BSA-C3G complex were noted, accompanied by a tendency for the microstructure to become more flattened. This study offers valuable insights into the influence of SMF on the assembly behavior and structural characteristics of proteins and anthocyanins.

International audience | The human intestine is home to a complex bacterial community known as the gut microbiota. This microbiota performs functions that are essential for maintaining our health, and has been shown to influence the host's physiology and metabolism. As a result, dysregulation of this intestinal microbiota, known as dysbiosis, could be implicated in the development of various diseases. However, studies analysing the microbiota rarely assess causality, which can be demonstrated by using germ-free (GF) animals and transplanting microbiota. Using these strategies, it has been shown that the intestinal microbiota may play a causal role in the development of obesity and associated metabolic disorders, particularly liver diseases, including Metabolic dysfunction–associated steatotic liver disease (MASLD) and Alcoholic liver disease (ALD). Indeed, by transplanting the gut microbiota from mice with or without MASLD to GF mice, we showed that the propensity to develop MASLD features including hyperglycaemia or steatosis is transmissible by the gut microbiota. Regarding ALD, we first showed that a specific dysbiosis is present in alcoholic patients with a severe alcoholic hepatitis. GF mice colonized with the gut microbiota from a patient with severe alcoholic hepatitis (AH) developed more severe liver inflammation and higher liver necrosis, than GF mice colonized with the gut microbiota from an alcoholic patient without AH.In conclusion, we demonstrated that the individual susceptibility to liver diseases is driven, at least partly, by differences in intestinal microbiota composition. Our findings open new avenues for diagnostic procedures based on personalised medicine by the identification of patients at high risk of developing liver diseases. They also open new possibilities for liver diseases prevention and treatment through intestinal microbiota manipulation.

peer reviewed | Soybean is a crucial component of animal feed, especially for poultry and monogastric animals, because of its high protein and amino acid content. However, dependence on soybeans—particularly imported varieties—poses challenges, including price volatility and concerns regarding GMOs. Organic farming, which prohibits GMOs, faces even higher costs to source non-GMO soybean meal. To address these issues, alternative protein sources such as insects are attracting interest. Insects provide a rich supply of proteins, fats, vitamins, and minerals, offering a sustainable substitute for both soybean and fishmeal. Key insect candidates for feed include the black soldier fly, housefly, and yellow mealworm (Tenebrio molitor, TM). Research indicates that insect meal can replace traditional feed sources without reducing productivity. This study, conducted at a poultry farm in Chemini, Bejaia, Algeria, examined 36 Isa-Brown laying hens, 40 weeks old. The hens were divided into 12 pens (3 control and 9 experimental), with experimental groups receiving feed supplemented with 1% (TM1), 2% (TM2), or 4% (TM4) mealworms. Over a four-week period, egg quality (physical and chemical properties) and feed conversion efficiency were evaluated. Data analysis, performed using SAS software with a significance threshold of P<0.05, revealed no significant differences in laying rates (P=0.48), although the TM4 group showed a slightly lower rate. However, TM4 achieved the best feed conversion ratio (P=0.03). Egg quality parameters, including weight and shell characteristics, remained consistent across all groups, with no significant differences in cholesterol or essential fatty acids. Incorporating mealworms into poultry feed thus appears to improve feed efficiency while maintaining egg quality, although there was a slight reduction in laying performance at higher inclusion rates. The relatively small sample size, however, limits the generalizability of these findings. | Le soja est un composant essentiel de l'alimentation animale, en particulier pour la volaille et les animaux monogastriques, en raison de sa teneur élevée en protéines et en acides aminés. Cependant, la dépendance au soja – en particulier aux variétés importées – pose des défis, notamment en termes de volatilité des prix et de préoccupations liées aux OGM. L’agriculture biologique, qui interdit les OGM, se heurte à des coûts encore plus élevés pour se procurer du tourteau de soja non-OGM. Afin de résoudre ces problèmes, des sources alternatives de protéines, telles que les insectes, suscitent de plus en plus d'intérêt. Les insectes offrent un apport riche en protéines, lipides, vitamines et minéraux, constituant une alternative durable au soja et à la farine de poisson. Parmi les insectes les plus prometteurs pour l’alimentation animale figurent la mouche soldat noire, la mouche domestique et le ver de farine jaune (Tenebrio molitor, TM). La recherche indique que la farine d'insectes peut remplacer les sources d'alimentation traditionnelles sans diminuer la productivité. Cette étude, menée dans une ferme avicole à Chemini, Béjaïa, en Algérie, a examiné 36 poules pondeuses Isa-Brown âgées de 40 semaines. Les poules ont été réparties dans 12 enclos (3 enclos témoins et 9 expérimentaux), les groupes expérimentaux recevant un aliment enrichi en vers de farine à raison de 1 % (TM1), 2 % (TM2) ou 4 % (TM4). Sur une période de quatre semaines, la qualité des œufs (caractéristiques physiques et chimiques) et l'efficacité de conversion alimentaire ont été évaluées. L'analyse des données, effectuée à l'aide du logiciel SAS avec un seuil de signification de P<0,05, n'a révélé aucune différence significative dans les taux de ponte (P=0,48), bien que le groupe TM4 ait montré un taux légèrement inférieur. Cependant, le groupe TM4 a obtenu le meilleur indice de conversion alimentaire (P=0,03). Les paramètres de qualité des œufs, y compris le poids et les caractéristiques de la coquille, sont restés constants dans tous les groupes, sans différences significatives au niveau du cholestérol ou des acides gras essentiels. L'incorporation de vers de farine dans l'alimentation des poules pondeuses semble donc améliorer l'efficacité alimentaire tout en maintenant la qualité des œufs, bien qu'une légère réduction de la performance de ponte ait été observée aux taux d'inclusion les plus élevés. Toutefois, la taille relativement réduite de l'échantillon limite la généralisation de ces résultats. | يُعتبر فول الصويا مكونًا أساسيًا في تغذية الحيوانات، وخاصةً الدواجن والحيوانات أحادية المعدة، نظرًا لمحتواه العالي من البروتين والأحماض الأمينية. ومع ذلك، فإن الاعتماد على فول الصويا – خاصةً الأصناف المستوردة – يطرح تحديات، بما في ذلك تقلب الأسعار والمخاوف المتعلقة بالكائنات المعدلة وراثيًا (OGM). كما تواجه الزراعة العضوية، التي تحظر استخدام الكائنات المعدلة وراثيًا، تكاليف أعلى للحصول على كُسب فول الصويا غير المعدل وراثيًا. وللتغلب على هذه المشكلات، تكتسب مصادر البروتين البديلة، مثل الحشرات، اهتمامًا متزايدًا. فالحشرات تُقدم مصدرًا غنيًا بالبروتينات والدهون والفيتامينات والمعادن، مما يجعلها بديلاً مستدامًا لفول الصويا وطحين السمك. ومن بين الحشرات الواعدة لتغذية الحيوانات نجد الذبابة الجندية السوداء، وذبابة المنزل، ودودة الطحين الصفراء (تينبريو موليتور، TM). وتشير الأبحاث إلى أن طحين الحشرات يمكنه استبدال المصادر الغذائية التقليدية دون التأثير على الإنتاجية. أُجريت هذه الدراسة في مزرعة دواجن بمنطقة شَمّيني، ولاية بجاية، الجزائر، واشتملت على 36 دجاجة بياضة من نوع Isa-Brown بعمر 40 أسبوعًا. تم توزيع الدجاجات على 12 حظيرة (3 حظائر كمجموعة ضابطة و9 حظائر كمجموعات تجريبية)، حيث تلقت المجموعات التجريبية علفًا مُضافًا إليه ديدان الطحين بنسبة 1% (TM1)، 2% (TM2) أو 4% (TM4). وعلى مدار فترة أربعة أسابيع، تم تقييم جودة البيض (الخصائص الفيزيائية والكيميائية) وكفاءة تحويل العلف. أظهرت نتائج التحليل الإحصائي، الذي أُجري باستخدام برنامج SAS مع مستوى دلالة P<0.05، عدم وجود فروق ذات دلالة إحصائية في معدلات البيض (P=0.48)، على الرغم من أن مجموعة TM4 أظهرت معدلًا أقل قليلًا. ومع ذلك، حققت مجموعة TM4 أفضل معدل تحويل غذائي (P=0.03). بقيت خصائص جودة البيض، بما في ذلك الوزن وخصائص القشرة، متناسقة بين جميع المجموعات دون فروق ذات دلالة إحصائية في مستويات الكوليسترول أو الأحماض الدهنية الأساسية. يبدو أن إدماج ديدان الطحين في علف الدجاج البياض يحسن كفاءة التغذية مع الحفاظ على جودة البيض، رغم حدوث انخفاض طفيف في أداء وضع البيض عند معدلات الإضافة الأعلى. ومع ذلك، فإن حجم العينة الصغير نسبيًا يحد من إمكانية تعميم هذه النتائج.