A 12-year-old 4.3 kg intact female Terrier presented with a history of lethargy, anorexia, and melena for one month. The animal was dull and depressed. Dermatologic examination revealed some focal erythematous, crusty, and papulopustular lesions over the ventral abdomen. Ultrasonographic evaluation displayed an abnormal parenchymal pattern of the liver, which was noticed in the cranial part of the abdomen with diffuse hepatic involvement with irregular, hypoechoic, and heterogeneous ill-defined areas [a honeycomb-like echotexture]. Lateral thoracocervical radiograph showed numerous well-defined, small lytic lesions [polyostotic punched-out lesions] in the dorsal spinous process of axis bone in C2-C5 and pelvis that were likely metastatic lesions. Despite hospitalization and treatment, the dog died after 2 weeks and a necropsy was performed. At postmortem examination, a 5-cm mass was observed in the pancreas adjacent to duodenal loops which was finally diagnosed as a pancreatic tumor. Abnormal laboratory findings included elevated blood urea nitrogen, creatinine, cholesterol, total bilirubin, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyltranspeptidase, and calcium. Histopathological examination of the affected pancreas revealed neoplastic cells which were arranged mainly as solid nests or clusters and the amounts of the tumor stroma among the cellular clusters or individual neoplastic cells were scant. According to an immunohistochemical study which was positive for chromogranine A, the condition was diagnosed as a pancreatic islet cell tumor. Although rare, pancreatic islet tumors should be included in the differential diagnosis of abdominal discomforts, pancreatic inflammation, and hepatopathies. The final diagnosis of the tumor is achieved by combining imaging techniques and advanced histopathologic evaluations.

This research paper presents a comprehensive morphologic and morphometric analysis of the forelimb bones (scapula, humerus, radius, and ulna) of the common eland (Taurotragus oryx) that are facing the threat of extinction. The study aims to contribute essential anatomical and clinical data, as well as facilitate biomedical research and educational endeavors. Bone specimens sourced from the Bangladesh National Zoo, Dhaka, were subjected to a standard hot water maceration technique, followed by sun-drying and treatment with 5% hydrogen peroxide. The scapula displayed a distinct triangular flat shape with a well-developed spine, and demarcating supraspinous and infraspinous fossae. Scapular weights were recorded at 698 gm (right) and 718 gm (left). The humerus, a robust and elongated bone measuring an average of 35.9 cm, exhibited a compressed cylindrical diaphysis and expanded epiphyses, with the right humerus weighing 944 gm and the left, 1016 gm. Notably, the humeral shaft displayed distinct regional compressions and expansions. The radius, slightly oblique and broader than the ulna, demonstrated cranial-caudal flattening. In contrast, the ulna, the longest bone at an average of 41.6 cm in the forearm, displayed distinct anatomical features. These findings offer valuable insights for comparative anatomical investigations within this taxonomic group and hold significance for clinical applications and educational purposes.

Chlamydiosis is an important disease in sheep, camel, goats, cats, birds, and cattle, which is caused by different species of the genus Chlamydia. Chlamydiosis of ruminants is a zoonosis and is especially worrying for pregnant women in contact with animal shelters. Chlamydiosis in camels can cause abortion, cervical adhesion, ovarian hydrobursitis, and reproductive failure in male camels. Chlamydia in camels can remain asymptomatic for a long time. Infected camels may play an important role in the transmission of Chlamydia to other animal species. Chlamydiaceae family members are currently placed in one genus and seven species. Among these seven species, Chlamydia abortus is of special importance in camels. It is possible to detect and distinguish chlamydial species by PCR and specific primers. The present study is the first study of Chlamydia abortus in the Iranian dromedary camel, which is very important. A total of 100 blood samples with anti-coagulant were taken from apparently healthy male and female camels in the south of Kerman province. Next, DNA was extracted from each blood sample using a blood DNA extraction kit according to the manufacturer's instructions. PCR was performed using rOMP90_3 specific primer to evaluate the presence of Chlamydia abortus. None of the samples were positive for Chlamydia abortus. According to the results, it can be said that Chlamydia abortus is probably not common in camels in the south of Kerman province of Iran.

Peganum harmala contains pharmacologically active compounds and has been utilized for various purposes over the years. Due to public health concerns about snakebite envenoming, this study aimed to assess the potential antagonistic effects of this plant against the lethal impact of snake (Naja naja oxiana) venom. This study used five protocols and 56 adult albino mice in seven equal groups (A, B1, B2, C, D, E, and F). In protocol I (control), group A received only 4 mg/kg of venom, while groups B1 and B2 received the P. harmala extract at doses of 15 and 30 mg/kg, respectively. In protocol II, group C was simultaneously administered 15 mg/kg of the extract and 4 mg/kg of venom. In protocol III, group D received 4 mg/kg of venom, followed by the administration of 15 mg/kg of the extract after 20 min. In protocol IV, group E was treated with venom-extract pre-incubated for 20 min at the same doses. In protocol V, group F received 30 mg/kg of the extract orally 60 min before the injection of venom at 4 mg/kg. The route of injection was IP. The average time of death after venom injection was 31 ± 5 min. Groups B1 and B2 survived, while the animals in group C died after 29 ± 7 min, group D after 18 ± 4 min, group E after 17 ± 5 min, and group F after 22 ± 3 min. In conclusion, P. harmala does not protect against Naja naja venom and accelerates its lethal effect in an unknown way.

HS is still a frequently reported endemic disease, with outbreaks in Indonesia. HS vaccines distributed in Indonesia exhibit various limitations. This study computationally evaluated the B-cell epitope of the 37-kDa OmpH derived from the amino acid sequence of Pasteurella multocida from the NTT and Katha strains and compared the epitopes of the two strains. Amino acid sequences were obtained from NCBI and analyzed for multiple sequence alignment, and homology was analyzed using the BLASTp program at NCBI. Epitope prediction was performed using the IEDB B-cell epitope and ABCPred prediction tools. The VaxiJen v.2 online platform was used for antigenicity analysis, and IEDB was used for epitope conservancy analysis. The results of the homology analysis revealed that local NTT isolates had a high (>95%) identity with the Katha strain and isolates from China, India, Iran, Japan, and the USA. The epitope predictions from both methods were cross-checked, overlapping epitopes were shortlisted, and only five epitopes were predicted. Among the five, one epitope, ALEVGLN, appeared to be antigenic to both NTT and Katha strains. The antigenic sequence of 37 kDa OmpH can be used for peptide-based vaccine development and immunotherapeutic design.

FIP is a systemic infectious disease of cats of coronavirus origin. The lack of clear signs of the presence of the virus before clinical form presentation, and the absence of easy and inexpensive diagnostic tests to confirm virus presence are among the problems for controlling and preventing the spread of the virus. In addition, there is not yet any approved medications or treatment protocols for this disease. In this paper, the gene co-expression  network was first reconstructed and modulated using the STRING database and Cytoscape software. The GO and pathways of the modules were obtained using the DAVID and KEGG databases. The most important possible pathways are proteasome, protein processing in the endoplasmic reticulum, protein export, aminoacyl-tRNA biosynthesis, phagosome, tuberculosis, and T cell receptor signaling pathway. In the other part of the study, the gene-drug network regeneration strategy was used to identify a potential medicine reconstructed using the DGIdb database and Cytoscape software using the drug-gene network. BORTEZOMIB, CARFILZOMIB, OPROZOMIB, IXAZOMIB CITRATE, MARIZOMIB, BCG VACCINE, IC14, NELFINAVIR, and RITONAVIR are some of our recommended drugs for this disease. Although our computational strategy predicts repurposable candidate drugs against FIP, more detailed experimental trials and clinical analyses of drug performance, toxicity, and validation are necessary to achieve an accurate and improved treatment protocol.

Apple cider vinegar is a fermented compound that contains acetic acid, flavonoids, phenolic compounds, organic acids, minerals, and vitamins. Vitamin A is involved in the development of the immune system and plays regulatory roles in cellular immune responses and humoral immune processes. The aim of this study was to investigate the effect of apple cider vinegar and vitamin A on hematological parameters and immunoglobulin G levels in Gezel lambs. 10 healthy Gazel lambs were used for the study. The lambs were randomly grouped into three groups (control (n = 3), Apple cider vinegar (n = 4), and Vitamin A (n = 3) group). Vitamin A was administrated at a dose of 44,000 IU/kg every ten days for four treatments. ACV was administered orally by drenching (0.5 ml/kg of 6% ACV solution (600 mg ACV), every day, for 40 days). Hematological parameters were determined using standard methods. Total immunoglobulin G concentration was assayed using the turbidimetric immunoassay method. The data obtained before and after drug administration were analyzed by paired T-test and the data of different groups were analyzed using Independent-sample T-test.White blood cells, lymphocytes, and IgG in lambs were significantly increased after administration of Apple cider vinegar (p < 0.05). IgG and lymphocytes were significantly higher in lambs under oral administration of Apple cider vinegar compared to the lambs in the control group (p  < 0.05). White blood cells, neutrophils, and IgG were significantly increased in lambs after vitamin A administration (p < 0.05). Neutrophils and IgG were significantly higher in lambs under vitamin A injection compared to the lambs in the control group (p < 0.05). Administration of vitamin A and apple cider vinegar in sheep is safe. They also improve the immune system.

This study aimed to evaluate the effects of silymarin on diclofenac-induced acute liver and kidney poisoning in domestic pigeons (Columba livia). The use of NSAIDs leads to adverse drug effects, such as cardiovascular and gastrointestinal hemorrhage and renal side effects. The vast amount of pharmacological attributes possessed by silymarin describes the remarkable content of research aimed at understanding its effect in the remedy of diverse diseases. Fifteen pigeons were randomly assigned into three groups (1, 2, and 3). Group 1 pigeons served as the negative control group and only were given tap water. Groups 2 and 3 were administered diclofenac (15 mg/kg PO q12h) since the start of the study for 24 h. The third group of pigeons was treated with silymarin (35 mg/kg) plus diclofenac, beginning 12 hours after diclofenac exposure, with the silymarin treatment continuing q12h for 48 h. Blood samples were taken from the birds at times 0, 12 h, 24 h, and 48 h of the experiment for serum biochemistry analysis. The results indicated that the treatment of pigeons with silymarin reduced the serum level of AST, ALT, UA, and urea while increasing ALB and TP. Clinical observations also indicated the presence of toxication symptoms, including loss of appetite, diarrhea, and lethargy. These symptoms improved faster in the silymarin group. It can be concluded that silymarin reduces acute liver and kidney damage caused by diclofenac in pigeons.

Diabetes is one of the most common metabolic diseases worldwide which affects all organs, including the reproductive system. Today, many researchers use medicinal plants instead of chemical agents to achieve fewer side effects. Ferula assa-foetida L. is one of the medicinal plants used to treat many diseases traditionally for years. The present study evaluated the antidiabetic and protective effects of Ferula assa-foetida L. on the testis of streptozotocin-induced diabetic male rats. The histomorphologic study of diabetic rats treated with Ferula assa-foetida L. extract showed a significant improvement in testes. Histological studies revealed that treatment with the Ferula assa-foetida L. extract significantly increased sperm count in the seminiferous tubules and reduced fibrosis. Our study confirmed the improving effects of Ferula assa-foetida L. on histomorphometric and biochemical parameters in diabetes and related testicular damage, which are partially attributed to the bioactive compounds and antioxidants in Ferula assa-foetida L.

This study explored the potential contribution of Orx1R within vlPAG to the learning and memory of animals with chronic migraine-like pain. Migraine was induced by repeated i.p. administration of nitroglycerin (5 mg/kg). Passive avoidance adeptness was evaluated in the shuttle box maze. The spatial memory performance was estimated using MWM tests. In the MWM task, NTG-injected rats revealed an imperative increase in escape latency and traveled the distance to catch the stage and a decrease in the time spent to pass into the goal zone in comparison to the control animals. Such NTG-evoked responses were attenuated by the post-treating intra-vlPAG injection of orexin A at 100 but not 25 and 50 pM. Furthermore, in the shuttle box test, NTG-treated rats showed eversion memory retrieval impairment as reflected by decreased phase through latency and longer time spent in the black chambers of the maze. Administration of orexin A at 50 and 100 pM could suppress NTG-related eversion memory deficiency in rats. However, orexin A (100 pM) aptitude to preserve memory performance, in both MWM and shuttle box tasks, was significantly prevented by SB334867 (20 nM) as an Orx1R antagonist. Overall, these data support the role of Orx1R within vlPAG to modulate migraine-related cognition deficits in rats.