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Analysis of genetic diversity for cattle parentage testing using microsatellite markers
Cho, G.J.;Yang, Y.J.(Korea Racing Association, Gwachon, Republic of Korea)E-mail:chogj@kra.co.kr | Lee, K.W.(Miryang National University, Miryang, Republic of Korea)
The objective of present study was to ascertain genetic diversity for cattle parentage testing. A total of 59 random cattle samples(29 Korean native cattle and 30 dairy cows) were genotyped by using 11 microsatellite loci(BM1824, BM2113, ETH10, ETH225, EH3, INRA23, SPS115, TGLA122, TGLA227, TGLA53, and TGLA126). This method consisted of multiplexing PCR procedure and showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer.
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