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Antibiotic resistance pattern of Enterococcus spp. and Staphylococcus aureus isolated from chicken feces
2005
Lee, Y.J. (Kyungpook National University, Daegu, Republic of Korea), E-mail: youngju@mail.knu.ac.kr | Kim, A.R. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Jung, S.C. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Song, S.W. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kim, J.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea)
This study was carried out to investigate the antibiotic resistance pattern of Enterococcus spp. and Staphylococcus aureus (S. aureus) isolated from chicken feces. All isolates showed high resistance to erythromycin (E) and tetracycline (TE). Of the 63 Enterococcus faecalis (E. faecalis) isolates, 73.0% were resistant to E and 98.4% to TE. Of the 44 Enterococcus faecium (E. faecium) isolates, 50.0% were resistant to E and 95.5% to TE. Of the 52 S. aureus isolates, 57.6% were resistant to E and 96.2% to TE. The prevalence of two and three drugs resistance pattern were 28.6% and 17.5% of E. faecalis, 40.9% and 25.0% of E. faecium and 38.5% and 23.1% of S. aureus, respectively.
Afficher plus [+] Moins [-]Patterns of antibiotic resistance in Escherichia coli isolated from fresh and recycled poultry litter
2017
Sung, H.W., Kangwon National University, Chuncheon, Republic of Korea | Choi, K.S., Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Kwon, H.M., Kangwon National University, Chuncheon, Republic of Korea | Lee, Y.J., Kyungpook National University, Daegu, Republic of Korea
The isolation rate of Escherichia (E.) coli in poultry litter was investigated at 44 broiler farms, 20 that used fresh litter and 24 that used recycled litter. The patterns of resistance to antibiotics of the E. coli isolates were compared. In litter sampled before the rearing period, the isolation rate of E. coli was higher at farms that used fresh litter; E. coli was present in the litter in 94.5% (35 out of 37 flocks tested) of the farms that used fresh litter vs. 51.2% (21 out of 41 flocks) of the farms that used recycled litter. The susceptibility of the 93 isolates of E. coli to 13 antibiotics was studied. Before the rearing period, E. coli isolates from the farms that recycled litter showed higher resistance rates than isolates from farms that replaced litter with fresh litter. Comparing the antibiotic resistance patterns of isolates from litter sampled before and at the end of the rearing period, the antibiotic resistance rates at the end of the rearing period increased dramatically compared with rates before the rearing period.
Afficher plus [+] Moins [-]First isolation of Salmonella I 4,[5],12:i:- from domestic animals in Korea
2012
Lee, D.Y., National Institute of Health, Korea Center for Disease Control and Prevention, Cheongwon, Republic of Korea | Kang, M.S., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Kwon, Y.K., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | An, B.K., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Kim, Y.J., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Heo, E.J., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Moon, J.S., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Lee, Esther, National Institute of Health, Korea Center for Disease Control and Prevention, Cheongwon, Republic of Korea | Park, H.M., National Institute of Health, Korea Center for Disease Control and Prevention, Cheongwon, Republic of Korea
Salmonella I 4,[5],12:i:- was a monophasic variant of Salmonella (S.) Typhimurium and notorious for reemerging candidate which would replace S. Typhimurium DT104 for antibiotic resistance. Recently, isolation rate was increased on human and industrial animals but there was no case in domestic animals but human in Korea. This was first isolation case from domestic animals in Korea. The five isolates from feces of duck (n = 3), chicken (n = 1), and wild bird (n = 1) showed antibiotic resistance against cephems and aminoglycosides. These means that the spread of emerging bacterial pathogens to domestic animals and the need of systemic management for Salmonella I 4,[5],12:i:-.
Afficher plus [+] Moins [-]Antibiotic resistance pattern of E. coli and Salmonella spp. isolated from chicken feces
2005
Lee, Y.J. (Kyungpook National University, Daegu, Republic of Korea), E-mail: youngju@mail.knu.ac.kr | Kim, A.R. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Jung, S.C. (Kyungpook National University, Daegu, Republic of Korea) | Song, S.W. (Kyungpook National University, Daegu, Republic of Korea) | Kim, J.H. (Kyungpook National University, Daegu, Republic of Korea)
The purpose of this study was to investigate the antibiotic resistance pattern of E. coli and Salmonella spp. isolated from chicken feces. One hundred and forty-seven E. coli isolates showed resistance to tetracycline (95.2%), erythromycin (89.2%), ampicillin (70.1%), streptomycin (59.2%), cephalothin (56.5%), sulfamethoxazole/trimethoprim (53.7%), ciprofloxacin (57.1%), enrofloxacin (59.2%) and norfloxacin (57.1%). The multiple resistance was seen in 144 isolates (97.9%) and the rate of five, six and seven drugs resistance pattern were 20.4%, 18.4% and 16.3%, respectively. Also, the multiple resistance of E. coli to twelve drugs were seen in 1 isolates (0.7%).
Afficher plus [+] Moins [-]Identification of microbiome with 16S rRNA gene pyrosequencing and antimicrobial effect of egg white in bovine mastitis
2017
Kim, D., Seoul National University, Seoul, Republic of Korea | Kim, E.K., Seoul National University, Seoul, Republic of Korea | Seong, W.J., Seoul National University, Seoul, Republic of Korea | Ro, Y., Seoul National University, Seoul, Republic of Korea | Ko, D.S., Seoul National University, Seoul, Republic of Korea | Kim, N.H., Institutes of GreenBio Science Technology, Pyeongchang, Republic of Korea | Kim, J.H., Institutes of GreenBio Science Technology, Pyeongchang, Republic of Korea | Kwon, H.J., Seoul National University, Seoul, Republic of Korea
Bovine mastitis is an important microbial disease in the dairy industry. We investigated the frequencies of bacterial pathogens in 62 farms and pathogen antibiotic resistance from mastitis samples (n = 748). We tested the antimicrobial activity of chicken and duck egg white and lysozyme purified from chicken egg white. Moreover, we compared the microbiomes of normal and mastitic raw milk obtained by 16S rRNA gene pyrosequencing and culture methods. The results showed that the frequencies of Gram-positive pathogens (Enterococcus faecalis 37% and Staphylococcus aureus 36%) were higher than that of a Gram-negative pathogen (Escherichia coli 15%). Resistance frequencies to ampicillin and norfloxacin were lowest in Staphylococcus aureus (21%), Enterococcus faecalis (23%), and Escherichia coli (33%), and the antimicrobial activity of chicken egg white was higher than those of lysozyme and duck egg white. Pyrosequencing results revealed clear differences between the microbiomes of mastitic and normal raw milk samples and revealed a slightly similar, but clearly different, composition of pathogens compared to that from the culture method. Thus, pyrosequencing may be useful for elucidating changes in microbiomes during mastitis progression and treatment. A chicken egg white and antibiotic combination may help with mastitis treatment; however, further studies are needed.
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