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The anti-tumor effect of combined treatment with arsenic trioxide and interferone-α on transplanted murine Lewis lung carcinoma
2005
Seo, D.L. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, J.H. (Konkuk University, Seoul, Republic of Korea) | Yang, J.H. (Gyeongsang National University, Jinju, Republic of Korea) | Won, C.K. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, M.O. (Gyeongsang National University, Jinju, Republic of Korea) | Kwark, S.D. (Gyeongsang National University, Jinju, Republic of Korea) | Koh, P.O. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: pokoh@gsnu.ac.kr
In the present study, we expected the anti-tumor effect by combined treatment of arsenic trioxide and interferon (IFN)-α on murine Lewis lung carcinoma (LL2) cells through in vivo study. As a experimental model, LL2 cells (1×10∨6/mouse) were injected subcutaneously into the back region of mice. When the tumor volume reached 100 ㎣, mice were treated with 1 mg/kg arsenic trioxide, 50000 IU IFN-α, or arsenic trioxide and IFN-α. The development of tumor cells was significantly inhibited by combined treatment with arsenic trioxide and IFN-α. In arsenic trioxide and IFN-α treated group, apoptotic index was reached a peak valve at 48 hr after the treatment and it was restored to approximately the control level at 8 days.
Afficher plus [+] Moins [-]Inhibition of human breast carcinoma by BLC (Sargassum fulvellum) and BLC/HEN Egg in vitro and in vivo
2005
Jo, E.H. (Seoul National University, Seoul, Republic of Korea) | Park, H.K. (Sam-Hwa Farm, Yeongju, Republic of Korea) | Cho, S.D. (Seoul National University, Seoul, Republic of Korea) | Ahn, N.S. (Seoul National University, Seoul, Republic of Korea) | Jung, J.W. (Seoul National University, Seoul, Republic of Korea) | Yang, S.R. (Seoul National University, Seoul, Republic of Korea) | Park, J.S. (Seoul National University, Seoul, Republic of Korea) | Hwang, J.W. (Seoul National University, Seoul, Republic of Korea) | Lee, S.H. (Seoul National University, Seoul, Republic of Korea) | Park, J.R. (Seoul National University, Seoul, Republic of Korea) | Kim, S.J. (Seoul National University, Seoul, Republic of Korea) | Lee, Y.S. (Seoul National University, Seoul, Republic of Korea) | Kang, K.S. (Seoul National University, Seoul, Republic of Korea), E-mail: kangpub@snu.ac.kr
Much of the interest on the chemopreventive properties of herbs and plants has been raised, whereas little is regarding to anti-tumor effect of farming and aquatic products. In the present study, the anti-tumor effect of hot-water extract of a seaweed, BLC (Sargassum fulvellum) and BLC/HEN egg was investigated using MCF-7 cells in vitro and in vivo systems. We found that the BLC extract and BLC/HEN egg inhibited cell proliferation in a dose-dependent manner, which might be mediated through up-regulation of p53. Furthermore, this test compound can directly induce apoptosis in MCF-7 cells, which might be mediated through up-regulation of a pro-apoptotic Bax protein and down-regulation of a anti-apoptotic Bcl-2 protein, not by immune system.
Afficher plus [+] Moins [-]Cloning and characterization of cDNA encoding a prohibitin-like protein from Theileria orientalis
2005
He, W.(Hokkaido Univ., Sapporo (Japan)) | Ohashi, K. | Sugimoto, C. | Tsuji, M. | Onuma, M.
A cDNA clone encoding a prohibitin-like protein (Toprh) was isolated from a piroplasm cDNA library of Theileria orientalis and its nucleotide sequence was determined. An open reading frame, encoding a polypeptide of 278 amino acid residues, was found in Toprh cDNA sequence. An intron of 89 bp was identified when this cDNA clone was compared with the Toprh gene in the genome of T. orientalis. The deduced amino acid sequence of Toprh shares 93.8, 93.1 and 69.1% identities with the prohibitins of T. parva (from chromosome 1), T. annulata (from chromosome 1), and Plasmodium falciparum, (from chromosome 10), respectively. By Western blot analysis, Toprh was found to be expressed in the piroplasm stage of the parasites.
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