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Avidin-biotin complex for immunohistochemical diagnosis of Aujeszky's disease and hog cholera.
1990
Kim S.B. | Sur J.H. | Moon U.G.
Immunohistochemical diagnosis of Neospora caninum in tissue sections
1989
Lindsay, D.S. | Dubey, J.P.
An avidin-biotin-peroxidase complex immunoperoxidase staining method was developed to detect Neospora caninum in formalin-fixed, paraffin-embedded tissue sections. Specific antiserum to N caninum was made in rabbits and used to probe tissues from dogs naturally and experimentally infected with N caninum. The test detected tachyzoites and bradyzoites of N caninum. A reaction was not observed to Toxoplasma gondii, Hammondia hammondi, Sarcocystis cruzi, S capricanis, S tenella, Besnoitia jellisoni, Caryospora bigenetica, Hepatazoon canis, Atoxoplasma sp, or the organism causing canine dermal coccidiosis. When antiserum made in rabbits to T gondii was used in the test, reaction to N caninum was not observed.
Afficher plus [+] Moins [-]Immunohistochemical evaluation of intermediate filament expression in canine and feline neoplasms
1989
Moore, A.S. | Madewell, B.R. | Lund, J.K.
Specimens of neoplastic tissues from 19 dogs and 4 cats were examined immunohistochemically for intermediate filament expression, using commercially available antibodies. Staining was observed in a wide range of tumor tissues and in normal internal controls by use of antibodies to vimentin, desmin, glial fibrillary acidic protein, and low and high molecular weight cytokeratins. Intermediate filament expression was found to be consistent with light and/or electron microscopic findings, and hence believed to be an accurate indicator of tumor histogenesis in cats and dogs. Three fixatives were evaluated for their relative abilities to preserve antigenicity. Absolute alcohol was superior to B5 fixative and both were superior to formalin. Some tissues that clearly displayed intermediate filament antigens with alcohol and B5 fixative failed to stain when fixed in formalin.
Afficher plus [+] Moins [-]Labeled avidin-biotin enzyme-linked immunosorbent assay (LAB-ELISA) for detection of Japanese encephalitis antibody in swine sera
1984
Chang, H.C. | Ohkubo, Y. | Takashima, I. | Arikawa, J. | Hashimoto, N. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine)
Use of biotinylated antibody for the assay of Hanganutziu-Deicher antibodies and antigens in fluids and tissues from cancer patients
1989
Gathuru, J.K. (Yokohama Univ. (Japan). Faculty of Engineering) | Higashi, H. | Kato, S. | Usuba, O. | Naiki, M.
Development of diagnostic methods for rotavirus from pigs using monoclonal antibody
1992
Kang, S.Y. (Chungbuk Nat'l Univ., Chungbuk (Korea Republic). Coll. of Veterinary Medicine)
