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Devastating endosulfan poisoning in Korean native cattle
2013
Lee, B., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, H.K., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, K.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Yoon, S.S., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kim, M., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Park, J.W., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jeong, S.H., Gyeonggido Veterinary Service, Suwon, Republic of Korea | Lee, M.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Bae, Y.C., Animal and Plant Quarantine Agency, Anyang, Republic of Korea
Here, we report the poisoning case of 10 cows. Several distinct clinical signs such as convulsion, excessive salivation, circling, lateral recumbency, and death were observed. Necropsy and histopathological examination did not reveal any significant abnormal findings. Moreover, no bacteria or viruses were detected in tissue, blood, and feeding food. However, endosulfan was detected from the stomach contents and microbials. Our results strongly suggest that death of cows may be closely associated with endosulfan poisoning.
Afficher plus [+] Moins [-]The virulence of Brucella abortus isolated from cattle in Korea
2011
Lim, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, J.H., Northern Branch, Gyeongbuk Veterinary Service Laboratory, Andong, Republic of Korea | Kim, D.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, D.G., Gyeongsang National University, Jinju, Republic of Korea | Jun, M.H., Chungnam National University, Daejeon, Republic of Korea | Kim, S.H., Chungnam National University, Daejeon, Republic of Korea | Chang, H.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, H.J., Gyeongsang National University, Jinju, Republic of Korea | Min, W.G., Gyeongsang National University, Jinju, Republic of Korea | Kim, S., Gyeongsang National University, Jinju, Republic of Korea
In this study, we isolated 12 of Brucella (B.) spp. from cattle, which have been positive in Rose Bangal test and tube agglutination test in Gyeongbuk province in 2009. According to AMOS PCR analysis, isolated 12 strains were identified as B. abortus. Murine derived macrophage, RAW 264.7 cells, were infected with isolated 12 strains or reference strain (B. abortus 544), and bacterial internalization were characterized. According to these results, we divided the isolated strains into the following three groups: class Ⅰ, lower internalization than that of B. abortus 544; class Ⅱ, similar internalization to that of that of B. abortus 544; class Ⅲ, higher internalization than that of B. abortus 544 within RAW 264.7 cells. Furthermore, intracellular growth, bacterial adherent assay, LAMP-1 colocalization, virulence in mice and surface protein pattern were characterized. From these results, representative strains of class Ⅲ showed lower LAMP-1 colocalization, higher adherent efficiency, higher virulence in mice than those of B. abortus 544, and showed different pattern of surface proteins. These results suggest that B. abortus field strains, isolated from cattle in Korea, possess various virulence properties and higher internalization ability of field strain may have an important role for its virulence expression.
Afficher plus [+] Moins [-]Biological characterization of Brucella spp. isolated from cattle in Gyeongbuk, Korea
2010
Kim, J.H., Northern Branch, Gyeongbuk Veterinary Service Laboratory, Andong, Republic of Korea | Lim, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, D.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, D.G., Gyeongsang National University, Jinju, Republic of Korea | Jun, M.H., Chungnam National University, Daejeon, Republic of Korea | Kim, S.H., Chungnam National University, Daejeon, Republic of Korea | Chang, H.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, H.J., Gyeongsang National University, Jinju, Republic of Korea | Min, W.G., Gyeongsang National University, Jinju, Republic of Korea | Kim, S., Gyeongsang National University, Jinju, Republic of Korea
Members of the genus Brucella are facultative intracellular bacteria and cause brucellosis, a chronic disease in humans and abortion in animals. In this study, we tested sera for brucellosis of 15 Hanwoo farms in the western part of Gyeong-buk province, resulting 5 farms were brucellosis positive in 2008. We collected blood from 277 heads in the brucellosis positive 5 farms, and performed serological diagnosis, brucella positive cattle which had shown higher than 200 antibody titer in tube agglutination test were slaughtered, supramammary lymph nodes were collected, and Brucella spp. wild type isolation and identification were performed. From these results, 15 of Brucella spp. wild type strains were isolated and all strains were identified as B. abortus biotype 1 by biological and molecular analysis. In the antimicrobial susceptibility test, all 15 strains had a similar susceptibility and resistance pattern. This study may be useful for bacteriological and epidemiological understanding of cattle brucellosis in Korea.
Afficher plus [+] Moins [-]Characterization of Akabane virus (KV0505) from cattle in Korea
2008
Yang, D.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: yangdk@nvrqs.go.kr | Kim, Y.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, B.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kweon, C.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoon, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Song, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, S.H. (Jeju Veterinary Research Institute, Jeju, Republic of Korea)
Akabane disease is caused by an arthropod-borne viral pathogen and leads congenital abnormalities of the central nervous system in infected ruminants. One isolate, KV0505, showed cytopathic effect in Vero cells. The KV0505 isolate was obtained from plasma, which was collected from a cattle raised on Jeju Island in May 2005. Jeju Island is located near the southern part of the Korean peninsula. The isolate was confirmed as Akabane virus (AKAV) by immunofluorescence assay using AKAV specific monoclonal antibodies and reverse transcription polymerase chain reaction (RT-PCR). Suckling mice inoculated with the isolate showed signs of paralysis and died within 10 days post-inoculation. Comparisons of the KV0505 N gene sequence with 39 other known AKAV strains revealed nucleotide homologies ranging from 83.6% (MP496 strain) to 99.7% (M171 strain). When compared with the K-9 strain, which was isolated from a cow in Korea in 1994, the nucleotide sequence homology with the N gene was 99.7%. Thus, genes of the KV0505 isolate were closely related to those of the M171 strain, which were clustered into the Ic group of AKAV.
Afficher plus [+] Moins [-]Study of plasma beta-carotene concentration in dairy cows
1999
Nam, H.M. | Moon, J.S. | Joo, Y.S. (National Veterinary Research & Quarantine Service, Anyang (Korea Republic).) | Oh, T.H. | Park, Y.H. | Han, H.R. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
This study was carried out to determine the effects of beta-carotene on the control of mastitis in dairy cows during the dry period. The relationship between the levels of plasma beta-carotene and the status of udder health in Holstein dairy cows were investigated. Blood samples were collected from 117 cows to compare the levels of plasma beta-carotene in lactating cows. The levels of plasma beta-carotene were 1.82 micro gram/ml in healthy cows(n=65) and 1.12 micro gram/ml in mastitic cows(n=52), respectively(p0.01). In the experiment to compare the level of plasma beta-caroten in the cows at different stages of lactation, the plasma beta-carotene levels were 1.73 micro gram/ml in lactating cows(n
Afficher plus [+] Moins [-]Evaluation of micronucleus frequency in cytokinesis-blocked lymphocytes of cattle in the vicinity of Uljin nuclear power station
Kim, S.R.;Kim, S.H.(Chonnam National University, Gwangju, Republic of Korea)E-mail:shokim@chonnam.ac.kr | Kang, C.M.(Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea)
Cytogenetic and hematological analysis was performed in peripheral blood of cattle in the vicinity of Uljin nuclear power station and control area. The frequency of micronuclei(MN) in peripheral blood lymphocytes from cattle was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the bovine lymphocytes exposed in vitro to radiation over the range from 0 Gy to 4 Gy.
Afficher plus [+] Moins [-]Seroprevalence of Coxiella burnetii infection in cattle on Ulleung Island, Korea
2018
Seo, M.G., Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Ouh, I.O., Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Kim, Y.H., Gyeongbuk Veterinary Service Laboratory, Daegu, Republic of Korea | Kim, J.K., Gyeongbuk Veterinary Service Laboratory, Daegu, Republic of Korea | Kwon, O.D., Kyungpook National University, Daegu, Republic of Korea | Kwak, D., Kyungpook National University, Daegu, Republic of Korea
We assessed the seroprevalence of Coxiella burnetii (C. burnetii) in cattle on Ulleung Island, Korea in a population-based follow up study for 4 years and determined the spatial distribution and risk factors associated with C. burnetii. The seroprevalence of C. burnetii was determined to be 1.4-2.0% during 2011-2014. Overall, nine cattle from three farms that tested seropositive showed C. burnetii antibody seroconversions between 2011 and 2014. The number of seropositive cattle was low, suggesting that movement of and contact between animals was possible risk factors for the transmission of C. burnetii.
Afficher plus [+] Moins [-]Development of inactivated Akabane and bovine ephemeral fever vaccine for cattle
2015
Yang, D.K., Viral disease division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kim, H.H., Viral disease division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jo, H.Y., Viral disease division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Choi, S.S., Viral disease division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Cho, I.S., Viral disease division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea
Akabane and bovine ephemeral fever (BEF) viruses cause vector - borne diseases. In this study, inactivated Akabane virus (AKAV)+Bovine ephemeral fever virus (BEFV) vaccines with or without recombinant vibrio flagellin (revibFlaB) protein were expressed in a baculovirus expression system to measure their safety and immunogenicity. Blood was collected from mice, guinea pigs, sows, and cattle that had been inoculated with the vaccine twice. Inactivated AKAV+BEFV vaccine induced high virus neutralizing antibody (VNA) titer against AKAV and BEFV in mice and guinea pigs. VNA titers against AKAV were higher in mice and guinea pigs immunized with the inactivated AKAV+ BEFV vaccine than in animals inoculated with vaccine containing revibFlaB protein. Inactivated AKAV+BEFV vaccine elicited slightly higher VNA titers against AKAV and BEFV than the live AKAV and live BEFV vaccines in mice and guinea pigs. In addition, the inactivated AKAV+BEFV vaccine was safe, and induced high VNA titers, ranging from 1 : 64 to 1 : 512, against both AKAV and BEFV in sows and cattle. Moreover, there were no side effects observed in any treated animals. These results indicate that the inactivated AKAV+BEFV vaccine could be used in cattle with high immunogenicity and good safety.
Afficher plus [+] Moins [-]Seroprevalence of Q-fever in Korean native cattle
2014
Kim, J.Y., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Sung, S.R., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Pyun, J.I., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Her, M., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kang, S.I., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, H.K., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jung, S.C., Animal and Plant Quarantine Agency, Anyang, Republic of Korea
Q-fever is a vector-borne (Coxiella [C.] burnetii) zoonotic disease that is an increasing public health concern. To date, some research about Q-fever prevalence in dairy herds and human patients has been reported in Korea, but information about Korean native cattle is scarce. To measure the prevalence rates of C. burnetii in Korean native cattle, a total of 1,095 bovine serum samples collected during 2010~2013 were analyzed with an enzyme-linked immunosorbent assay. Sixty-eight heads of cattle were diagnosed as positive and while 19 heads were suspected (positive rate = 6.2%). Interestingly, Jeju province had a seropositivity rate six times greater than that of other provinces (18.9% vs. 3.2%). High seroprevalence might be caused by wide distribution of ticks in Jeju province compared to other regions. Based on these data, extensive monitoring of C. burnetii infection in cattle, tick distribution, and climate changes is required.
Afficher plus [+] Moins [-]Epitheliogenesis imperfecta in a bovine fetus of Korean native cattle
2014
Rhyoo, M.Y., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jung, J.Y., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Her, J.W., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, M.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Ku, K.N., Institute for Gyeonggi-do Veterinary Service, Bucheon, Republic of Korea | Choi, K.R., Institute for Gyeonggi-do Veterinary Service, Bucheon, Republic of Korea | Yoon, S.S., Animal and Plant Quarantine Agency, Anyang, Republic of Korea
In this study, we describe a case of epitheliogenesis imperfecta (EI) observed in the fetus of Korean native cattle. The fetus had multifocal areas of skin defect, especially on the distal portions of the four limbs, and the affected areas were bright-red and glistening. Histopathologically, these areas were characterized by complete absence of squamous epithelium, infiltration of inflammatory cells into the dermis, atrophy of hair follicles, sebaceous and sweat glands. To the best of our knowledge, this is the first report of epitheliogenesis imperfecta in Korean native cattle.
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