Edible bird's nest (EBN)is made from the saliva of swiftlet from the Aerodramus species. It is one of the most widely consumed traditional health food by the Chinese community due to its claimed medicinal value. EBN contains glycoproteins with abundance of sialic acid (SA). EBN with higher SA content has a potential to command for higher price. The objective of this study was to determine and compare free SA content of EBN collected from Johor and Kelantan. A total of 23 and 30 of raw and unprocessed EBN samples were obtained from Kelantan and Johor, respectively. SA from EBN samples were analysed using LC-MS/MS. Johor showed higher content of free SA with the value of 135.04 ± 29.60 mg/kg compared to Kelantan which has a value of 95.55 ± 25.6 mg/kg. Highest content of free SA was found in EBN from Pontian district, Johor.

Balanced complete feeding proved to be one of the major conditions of animal productivity increase and improvement of their reproductive qualities. Full value of feeding could be obtained at the expense of forage quality improvement and perfection of structure of the rations providing the increasing of metabolic energy use by breeding bull-calves. Analysis of the efficiency of metabolic energy use by the replacement bull calves under conditions of various sugar and protein ratio was realized in the Republic of Belarus. Research results showed that enrichment of replacement bull calves (with 325-405 kg of live weight) rations with easy digestible carbohydrates (sugar and starch) in ratio of 32% from a dry matter ration, including 15% of stable starch made it possible to increase the transformation of metabolic energy into energy of body weight gain on 21,73 - 23,96 MJ or on 10,3%, that as a result correspondingly increased the daily average weight gains on 7,9% and reduced the expenses for forage energy for 7,1% counting on an energy unit. According to the ejaculate volume indices the bull calves of the second experimental group surpassed analogues of a control group on 14,8%, and according to sperm concentration indices - on 12%. The average quantity of the frozen doses of sperm has made 65%