A number of 50 Ardeola ibis ibis birds were found harboring six nematodes species; Tetrameres species, Microtteramere species, Synhimantus invaginatus, Synhimantus equispeculatus, Ascaridia species, Paracamallanus species,and five species of trematodes; Euclinostomum heterostomum, Nephrostomum ramosum, Apharyngostrigea ibis, Apatemon gracilis and Centrocestus armatus. The most common infection by nematodes was (46%) in which highest infection rate Synhimantus invaginatus recorded (30 %) while the trematode infection was (24 %) and Apatemon gracilis was the most prevalent (16 %). Experimental infection of buff backed heron by encysted metacercaria (EMC) and exysted metacercaria (ExMC) of Clinostomum complanatum from freshwater fish Tilapia nilotica, resulted in adult worms formed after 6 days. Where the infection by EMC recorded higher worm burden (14-18 worm) and hatching percent (78%) while the infection by ExMC gave lower worm burden (7-10 worm / bird ) and hatching (48 %). In the present study, it is worthy to mention that buff backed heron act as final host model for Clinostomum complanatum and this will be helpful in further biological and immunological studies for this trematode to decrease its economic losses in fish intermediate host.Fifty random samples of Kareish cheese were collected from different localities in Bani-suef Governorate. All samples were examined chemically for acidity, salt and moisture percent and bacteriologicaly for the presence of Escherichia coli, Staphylococcus aureus, Enterococci, Bacillus cereus, Clostridium perfringens, Yersinia enterocolitica, Salmonella and Shigella species. The obtained results revealed that the mean values of acidity, salt and moisture % were 1.63 ± 0.095,3.55 ± 0.299 and 58.54 ± 0.599 in the examined kareish cheese samples, respectively.Escherichia coli, Staphylococcus aureus, Enterococci, Bacillus cereus, Clostridium perfringens were recovered from 16 (32%), 12(24%), 46 (92%), 25 (50 %) and 3 (6%) with a mean value of 4.86x102 ±4.21x10 2, 4.84x 10 5 ± 2.91x10 5, 3.74x10 6±1.55x10 6, 7.08x10 4±2.61x10 4 and 9.5x10 1 ± 7.37x10 1 of the examined samples , respectively. Yersinia enterocolitica could be isolated from 12% of the examined samples. Salmonella and Shigella species could not be detected in any of the examined samples. The isolated Escherichia coli were examined for serological identification, Enterotoxigenicity and the susceptibility of the isolated serovars to various chemotherapeutic agents. The public health significance and economical importance of the isolated organisms and the recommendations to be followed in the processing, handling and storage of such dairy product were discussed

In the present study, 135 samples were collected from different animal's including:75 samples were from milk and 60 samples were from cheese, 54 (40%) sample were foundto be harbored with Staphylococcus aureus. The rate of S. aureus isolates was 50% inbuffalo's cheese, 40.54% in buffalo's milk, 36.8% in cow's milk, and 33.33% in cow'scheese. 100% strains were Methicillin Resistance Staphylococcus aureus. The antibioticsensitivity test was determined against 8 common antibiotics by the agar disc diffusionmethod on Muller-Hinton agar. These antibiotics were amoxicillin (25mcg), ampicillin(10mcg), Oxacillin (1mcg), chloramphenicol (30mcg), erythromycin (15mcg), gentamycin(10mcg), methicillin (5mcg), and tetracycline (30mcg). S. aureus strains were screened byPCR for 16S rRNA and nuc genes. 49 out of 54 S. aureus isolates were yielded productswith molecular weight approximately (228 bp) corresponding to 16S rRNA gene, 42 out of54 isolates were give products with molecular weight approximately (270bp) correspondingto nuc gene, 22 and 4 out of 30 S. aureus isolates were give products with molecular weightapproximately (310bp and 509bp) corresponding to mecA and femA genes, respectively.

The study was performed to the study synergistic effect of Ethylene diamine tetra acetic acid  (EDTA) and Imipenem against Pseudomonas aeruginosa were  isolated from cheese at Hilla province. Cheese samples 70 were collected randomly from (Retail, supermarkets and dairy shops) in Hilla and were transported to the laboratory.     The result revealed (45%)   Pseudomonas aeruginous detecte were collected from cheese. These isolates were tested for disk diffusion method for susceptibility of  imipenem. Eight isolates  were  resistant , and  (84.3%) of Pseudomonas aeruginosa strain were sensitive  (84.3%)  to EDTA alone.     Also, the result showed of Synergistic EDTA- IMP disc diffusion against (8) isolate of Pseudomonas aeruginosa revealed (87.5%) isolates were sensitive. However; the The result showed significant (P < 0.05) of susceptibility to (8) P. aeruginosa isolates to imipenem with and without EDTA. In conclusion, according to the present study can use oxidant agent inhibition of growth bacteria specific P. aeruginosa such as ethylene diamene tetra acidic acid (EDTA) and can used in preserving food specific sold cheese.