The present study was conducted to study the effect of dietary supplementation of vitamin C and nano vitamin C on the serum biochemical profile of Japanese quail during the summer season. The experiment was carried out on 96 day-old Japanese quail chicks which were divided randomly into three treatment groups. Each treatment contained two replicates with sixteen birds per replicate. The experiment was carried out for a period of six weeks during the summer season in May and June. Feed and water were provided ad libitum to study the effect of the inclusion of vitamin C (200 mg/kg) and nano vitamin C (20 mg/kg) on serum biochemical profile of Japanese quail during the summer season. The study revealed that the mean serum levels of total protein, albumin, globulin, HDL cholesterol and SGPT/ALT increased significantly (P<0.01); whereas, serum levels of total cholesterol, LDL cholesterol and triglycerides decreased significantly (P<0.01) in treatment groups supplemented with nano vitamin C. No significant differences (P>0.05) were observed in serum SGOT/AST among treatment groups.