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Partial purification and characterization of bovine fibroblast interferon.
1988
Allen G.K. | Grothaus G.D. | Rosenquist B.D.
Bovine fibroblast interferon (BoF-IFN), produced in primary bovine embryonic kidney cell cultures after priming and infection with bluetongue virus, was purified by controlled pore glass (CPG) chromatography to a specific activity of 10(6) U/mg of protein, with 40% recovery of the original activity. The crude IFN was concentrated more than sevenfold during purification. This proved to be a relatively simple, practical method of obtaining sufficient quantities of partially purified natural BoF-IFN for further studies. The CPG-purified BoF-IFN was further concentrated by sequential ultrafiltration and was analyzed by sodium dodecyl sulfate/polyacrylamide-gel electrophoresis (SDS-PAGE). Interferon, recovered from denaturing conditions either by dialysis against phosphate-buffered saline solution or by dilution in cell culture medium containing 10% fetal bovine serum, migrated as a single stainable protein with molecular weight of 21,000 on analytic SDS-PAGE gels. Recovered IFN activity from preparative SDS-PAGE totalled 8.7% of that applied. Attempts to further purify CPG-purified BoF-IFN by zinc chelate affinity chromatography were unsuccessful.
Afficher plus [+] Moins [-]Study on the development of simultaneous-rapid screening methods for residues pesticides in livestock products
1997
Kim, J.S. | Kim, G.S. (Gyeonsang National University, Chinju (Korea Republic). College of Veterinary Medicine) | Hah, D.S. (Gyeongnam Provinical government Institute of Health and Environment, Chinju (Korea Republic))
