Diarrhoea in calves is considering a serious clinical problem inBangladesh. Calf is the back bone of the dairy industry. Diarrhoea in farm animals, especially in neonatal calves is one of the most challenging clinical syndromes encountered by large animal Veterinarypractitioners. The present investigation was conducted with the aimed to evaluate the efficacy of ciprofloxacin and gentamycin in the treatment of diarrhoea in neonatal calves. The studied was carried out for a period of six month from January 2013 to June 2013 in Kanaighat, Sylhet, Bangladesh. Calves (n=60) under 10 days of age with clinical signs of diarrhoea were randomly divided into two groups. One group was treated with intramuscular ciprofloxacin at a dose of 5 mg/kg once daily, while other group was treated withintramuscular (IM) gentamycin (dose of 5 mg/kg) once daily for three consecutive days. Calves evaluated clinically from the treatment and the recovery rates for the ciprofloxacin and gentamycin groups afterthree days of treatment were 73.33% and 66.67%, respectively. This trial revealed ciprofloxacine administered through IM route from the first day of the clinical sign observed was more efficacious in calfdiarrhoea compared to gentamycin.