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Histological and histometrical study of duodenum in mice after ingestion of Aspartame
2016
Hooshmand Abbasi, Reyhaneh | Tootian, Zahra | Sheibani, Mohammad Taghi | Fazelipour, Simin | Limouei, Hossein
BACKGROUND: One of the most artificial sweeteners is Aspartame which is commonly used in a variety of foodstuffs. Objectives: This study has been carried out in order to evaluate the effects of Aspartame on histology and histometry of duodenum. Methods: Forty female Balb/C mice, 21 days of age were selected and initial weight was determined. The treatment groups received 100, 200 and 400 mg/kg of Aspartame for 6 weeks, control group received only distilled water. At the end of experiment, the mice were reweighed. Then the duodenal tissue sections were prepared and stained with H&E. Besides the histological study, histometric data were collected by a light microscope equipped with Axiovision software. Results: The body weights in treatment groups (100, 200 and 400 mg/kg) were 5.57±1.18, 4.47±0.89 and 5.84±0.57 respectively, which in comparison with the control group (9.38±0.81) showed a significant difference (p<0.05). The histological study showed that the rate of destruction in the cells and mucosal structures, at the dose of 200 mg/kg compared to the dose of 100 and 400 mg/kg has been increased. In histometric aspect, abundance of duodenal villi, height of the villi and thickness of duodenal mucosa, only in the experimental group of 200 mg/kg were significantly increased compared to the control group (p<0.05). Whereas the width of villi (width of the apex, body and the base), in all of the experimental groups showed a significant decrease compared to the control group (p<0.05). The thickness of musculature of duodenum in experimental groups had no significant differences with the control group. Conclusions: Based on this study, it can be concluded that Aspartame can cause some histological and histometrical changes in duodenum.
Afficher plus [+] Moins [-]Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88
2016
Wang Kai | Qi Yu | Yi Shushuai | Pei Zhihua | Pan Na | Hu Guixue
Introduction: The aim of the experiment was to establish the enterotoxigenic Escherichia coli K88 (ETEC K88)-induced BALB/c mouse duodenum inflammation model. Material and Methods: Mice were administered different concentrations of E. coli K88 (1.0 × 107-109 CFU/mL) for 3 d by means of an esophageal catheter. Results: The results showed that the treated group expressed several significant clinical symptoms, such as reduced dietary demands and weight loss, an increased presence of IL-1α, TNF-α, and MPO in the peripheral blood, and some pathological changes in the duodenum. On the 6th-8th days, the body weight of the mice was the lowest. On the 8th day, there were significant differences in IL-1α, TNF-α, and MPO levels compared to the control group (P < 0.05), the gap between the duodenum mucous layer and the muscular layer had widened, the number of goblet cells was increased, and the inflammatory infiltrate and inflammation changes in the lamina propria and the mucous layer were the most obvious. Conclusion: The duodenum inflammation was the most severe on day 8; thus, the model was successfully established. In addition, varying concentrations of ETEC K88 did not significantly influence the duodenum inflammation (P > 0.05).
Afficher plus [+] Moins [-]Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88
2016
Introduction: The aim of the experiment was to establish the enterotoxigenic Escherichia coli K88 (ETEC K88)-induced BALB/c mouse duodenum inflammation model. Material and Methods: Mice were administered different concentrations of E. coli K88 (1.0 × 10⁷-10⁹ CFU/mL) for 3 d by means of an esophageal catheter. Results: The results showed that the treated group expressed several significant clinical symptoms, such as reduced dietary demands and weight loss, an increased presence of IL-1α, TNF-α, and MPO in the peripheral blood, and some pathological changes in the duodenum. On the 6ᵗʰ-8ᵗʰ days, the body weight of the mice was the lowest. On the 8ᵗʰ day, there were significant differences in IL-1α, TNF-α, and MPO levels compared to the control group (P < 0.05), the gap between the duodenum mucous layer and the muscular layer had widened, the number of goblet cells was increased, and the inflammatory infiltrate and inflammation changes in the lamina propria and the mucous layer were the most obvious. Conclusion: The duodenum inflammation was the most severe on day 8; thus, the model was successfully established. In addition, varying concentrations of ETEC K88 did not significantly influence the duodenum inflammation (P > 0.05).
Afficher plus [+] Moins [-]Comparison of the expression, activity, and fecal concentration of intestinal alkaline phosphatase between healthy dogs and dogs with chronic enteropathy
2016
Ide, Kaori | Kato, Kazuki | Sawa, Yuki | Hayashi, Akiko | Takizawa, Rei | Nishifuji, Koji
OBJECTIVE To compare expression, activity, and fecal concentration of intestinal alkaline phosphatase (IAP) between healthy dogs and dogs with chronic enteropathy (CE). ANIMALS 9 healthy university-owned Beagles and 109 healthy client-owned dogs (controls) and 28 dogs with CE (cases). PROCEDURES Cases were defined as dogs with persistent (> 3 weeks) gastrointestinal signs that failed to respond to antimicrobials and anti-inflammatory doses of prednisolone or dietary trials, did not have mechanical gastrointestinal abnormalities as determined by abdominal radiography and ultrasonography, and had a diagnosis of lymphoplasmacytic enteritis or eosinophilic gastroenteritis on histologic examination of biopsy specimens. Duodenal and colonic mucosa biopsy specimens were obtained from the 9 university-owned Beagles and all cases for histologic examination and determination of IAP expression (by real-time quantitative PCR assay) and activity (by enzyme histochemical analysis). Fecal samples were obtained from all dogs for determination of fecal IAP concentration by a quantitative enzyme reaction assay. RESULTS For dogs evaluated, IAP expression and activity were localized at the luminal side of epithelial cells in the mucosa and intestinal crypts, although both were greater in the duodenum than in the colon. Active IAP was detected in the feces of all dogs. Intestinal alkaline phosphatase expression and activity were lower for cases than for controls, and fecal IAP concentration for dogs with moderate and severe CE was lower than that for dogs with mild CE. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that dogs with CE had impaired IAP expression and activity. Additional research is necessary to elucidate the role of IAP in the pathogenesis of CE.
Afficher plus [+] Moins [-]Effects of the −791(C→T) mutation in the promoter for tumor necrosis factor alpha on gene expression and resistance of Large White pigs to enterotoxigenic Escherichia coli F18
2016
Liu, Ying | Dai, Chaohui | Sun, Li | Zhu, Guoqiang | Wu, Shenglong | Bao, Wenbin
Tumor necrosis factor alpha (TNF-α) plays an important role in the immune system. In this study, TNF-α expression was analyzed in 11 tissues of 8 piglets resistant to enterotoxigenic Escherichia coli (ETEC) F18 and 8 ETEC F18-susceptible piglets from the Large White breed. The expression levels of TNF-α were high in immune organs (spleen, lung, thymus, and lymph nodes). The levels were higher in ETEC F18-resistant piglets than in ETEC F18-susceptible piglets, with significant differences in spleen, kidney, thymus, lymph node, and duodenum (P < 0.05). The mutation TNF-α −791(C→T) and 3 genotypes (CC, CT, and TT) were identified. The TNF-α expression levels in the spleen, kidney, lymph nodes, and duodenum were significantly higher in the TT pigs than in the CC pigs (P < 0.05). Thus, TNF-α −791(C→T) has significant effects on mRNA expression and may regulate ETEC F18 resistance of weaning piglets. Therefore, the −791(C→T) mutation of the TNF-α gene could be considered an important potential genetic marker of ETEC F18 resistance.
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