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Evaluation of an enzyme-linked immunosorbent assay for detection of Eperythrozoon suis antibodies in swine.
1992
Hsu F.S. | Liu M.C. | Chou S.M. | Zachary J.F. | Smith A.R.
An ELISA was developed and tested to detect antibodies to Eperythrozoon suis in swine. Results were compare with those of the indirect hemagglutination (IHA) test. Antigen isolated from swine heavily infected with E suis was used for both tests. Comparison of the ELISA with the IHA test revealed a significant (P < 0.001) correlation between results. Of 114 samples obtained from 9 swine infected with E suis, 8 7.7% were seropositive (titer greater than or equal to 200) via the ELISA, and 80.7% were seropositive (titer greater than or equal to 20) via the IHA test. The sensitivity of the ELISA was greater than that of the IHA test. All blood samples obtained from specific-pathogen-free swine tested negative for E suis antibody. Cross-reactions were not observed between E suis antigen and antisera against various swine and cattle disease agents using ELISA. We concluded that the ELISA may be used for rapid and effective diagnosis of infection with E suis in swine.
Afficher plus [+] Moins [-]Detection of antibodies against infectious Borna disease virus - A comparison of three serological methods-
1992
Lee, D.S. (Cheju Nat'l Univ., Cheju (Korea Republic). Dept. of Veterinary Medicine)
Development of diagnostic methods for rotavirus from pigs using monoclonal antibody
1992
Kang, S.Y. (Chungbuk Nat'l Univ., Chungbuk (Korea Republic). Coll. of Veterinary Medicine)
Evaluation of a cell enzyme-linked immunosorbent assay for the detection of borna disease virus antibodies in experimentally infected animals
1992
Lee, D.S. (Cheju National University, Cheju (Korea Republic). Department of Veterinary Medicine)
